• Title, Summary, Keyword: 팔딱이

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Identification and characteristics of DDX3 gene in the earthworm, Perionyx excavatus (팔딱이 지렁이(Perionyx excavatus) DDX3 유전자의 동정 및 특성)

  • Park, Sang Gil;Bae, Yoon-Hwan;Park, Soon Cheol
    • Journal of the Korea Organic Resources Recycling Association
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    • v.23 no.1
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    • pp.70-81
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    • 2015
  • Helicases are known to be a proteins that use the chemical energy of NTP binding and hydrolyze to separate the complementary strands of double-stranded nucleic acids to single-stranded nucleic acids. They participate in various cellular metabolism in many organisms. DEAD-box proteins are ATP-dependent RNA helicase that participate in all biochemical steps involving RNA. DEAD-box3 (DDX3) gene is belonging to the DEAD-box family and plays an important role in germ cell development in many organisms including not only vertebrate, but also invertebrate during asexual and sexual reproduction and participates in stem cell differentiation during regeneration. In this study, in order to identify and characterize DDX3 gene in the earthworm, Perionyx excavatus having a powerful regeneration capacity, total RNA was isolated from adult head containing clitellum. Full length of DDX3 gene from P. excavatus, Pe-DDX3, was identified by RT-PCR using the total RNA from head as a template. Pe-DDX3 encoded a putative protein of 607 amino acids and it also has the nine conserved motifs of DEAD-box family, which is characteristic of DEAD-box protein family. It was confirmed that Pe-DDX3 has the nine conserved motifs by the comparison of entire amino acids sequence of Pe-DDX3 with other species of different taxa. Phylogenetic analysis revealed that Pe-DDX3 belongs to a DDX3 (PL10) subgroup of DEAD-box protein family. And it displayed a high homology with PL10a, b from P. dumerilii.

Identity of Two Earthworms Used in Vermiculture and Vermicomposting in Korea:Eisenia andrei and Perionyx excavatus (국내 양식 지렁이 Eisenia andrei와 Perionyx excavatus의 구분)

  • Yong Hong;Kim, Tae-Heung;Na, Young-Eun
    • Animal Systematics, Evolution and Diversity
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    • v.17 no.2
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    • pp.185-190
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    • 2001
  • Two vermiculture earthworms, Eisenia andrei Bouche, 1972 and Perionyx excavatus Perrier, 1872 are newly recorded to Korean fauna. E.andrei has no pale stripe on the intersegmental furrow zone and P.excavatus has clitellum in xiii-xvi. Descriptions of the species are provided in this paper, including illustrations of the ventral view, male pore region, and spermathecae.

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