• Title, Summary, Keyword: 토복령 추출물

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Quality Characteristics of Pork Kalbi Sauce Added with Smilax china L. Extract during Storage (토복령 추출물 첨가로 인한 돼지갈비 소스의 품질 특성)

  • Ahn, Joungjwa
    • Journal of Convergence for Information Technology
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    • v.9 no.12
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    • pp.166-174
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    • 2019
  • The present study was designed to study the antioxidant activity and physicochemical and microbiological characteristics of Pork Kalbi sauce added with Smilax china L. extract(0%, 0.25%, 0.5% and 0.75%) for 5 weeks. Total microbial counts were slightly higher in control and 0.25%-added group, therefore, more addition of Smilax china L. extract lowered total microbial counts. Alpha-diphenyl-β-picrylhydrazyl(DPPH) radical scavenging activity was significantly higher in 0.75%-added group compared to those in others(p<0.05). Also, thiobarbituric acid reactive substances(TBARS) was significantly lower in 0.5% and 0.75%-added groups compared to others(p<0.05). These results indicated that Smilax china L. extract addition increased the antioxidant activity of Pork Kalbi sauce and could improve the shelf-life during storage.

Antimicrobial activity against Propionibacterium acnes bacteria of Smilacis glabrae Rhizoma extracts (토복령 추출물의 여드름 균에 대한 항균활성)

  • Park, Jang-Soon;Kwon, Hye-Jin
    • Journal of the Korea Convergence Society
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    • v.8 no.12
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    • pp.425-430
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    • 2017
  • This study was conducted to measure the antimicrobial activity and antiseptic effect of Smilax china root extracts against Propionibacterium acnes and to develop natural antimicrobial extracts as an alternative for synthetic preservatives, which have recently been controversial. Extracts were obtained from dried Korean Smilax china root at room temperature using two solvents, distilled water and 95% EeOH, separately. According to the results of this study, the antimicrobial effect of Propionibacterium acnes 3314 and Propionibacterium acnes 3320 against Propionibacterium acnes was outstanding. In particular, the clear zone of the extract using distilled water showed the highest activity with 16.61 mm. As for the result of the collection of contaminated skin sample, the clear zone was 24.48 mm. This indicates that Smilax china root extracts show a high activity against skin flora as well as a high antimicrobial activity against Propionibacterium acnes. In conclusion, it is confirmed that Smilax china root extracts can be used as raw materials for cosmetics that have antimicrobial activity and antiseptic effect, and it is expected that Smilax china root extracts will be used as basic materials for the development of future natural preservatives.

Antioxidant activity and protective effects on oxidative DNA damage of Smilax china root (토복령의 항산화 및 산화적 DNA 손상억제 활성)

  • Jang, Tae-Won;Oh, Chang-Gun;Park, Jae-Ho
    • Journal of Applied Biological Chemistry
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    • v.61 no.2
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    • pp.109-117
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    • 2018
  • Recently, cancer incidence in modern society is increasing sharply. DNA damage is caused by intrinsic or extrinsic factors in the human body, cells protect themselves by defense mechanism against DNA damage. Also, Aberrant DNA and deficient DNA repair are closely associated with various diseases, including aging and cancer. Researchers are interested in search for proper materials to inhibition for DNA damage. As knew the side effects of synthetic antioxidant, some researches have been conducted about cancer prevention materials derived from nature. Root of Smilax china, in Liliaceae, is used detoxification and tumor treatments traditionally. However, studies on the inhibitory effect of DNA damage haven't progressed. In this study, antioxidant activity and protective effects on oxidative DNA damage of S. china root were confirmed, relationship between those activities and contents of phenolic compounds in plants were established. S. china root effectively removed 1,1-diphenyl-2-picryl-hydrazyl radicals and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid radicals. The quantification and identification of phenolic compounds were confirmed by high performance liquid chromatography analysis, its antioxidant activity was associated with some phenolic compounds. In addition, protective effects against hydroxyl radicals and ferrous ion-induced oxidative DNA damage were confirmed in plasmid DNA. In the cellular levels, S. china root suppressed the expression of ${\gamma}$-H2AX and p53 protein in NIH 3T3. Besides, S. china root suppressed H2AX and p53 mRNA levels. In conclusion, S. china root had the effect on DNA protection and antioxidant.

The Anti-Obesity Effect of Smilax china Extract (토복령 추출물의 항비만 활성)

  • Park, Jung Ae;Jin, Kyong-Suk;Kwon, Hyun Ju;Kim, Byung Woo
    • Microbiology and Biotechnology Letters
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    • v.42 no.4
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    • pp.354-360
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    • 2014
  • In this study, the anti-obesity activity of Smilax china methanol extract (SCME) was evaluated using a pancreatic lipase enzyme inhibition assay, and a cell culture model system. Results indicated that, SCME effectively inhibited pancreatic lipase enzyme activity in a dose-dependent manner. Furthermore, SCME significantly suppressed insulin, dexamethasone, 3-isobutyl-1-methylxanthine-induced adipocyte differentiation, lipid accumulation, and triglyceride contents on 3T3-L1 preadipocytes, in a dose-dependent manner. The anti-adipogenic effect was modulated by cytidine-cytidine-adenosine-adenosine-thymidine (CCAAT)/enhancer binding proteins (C/EBP) ${\alpha}$, $C/EBP{\beta}$, and the peroxisome proliferator-activated receptor ${\gamma}$ gene and protein expressions. Moreover, SCME triggered lipolysis effects dose-dependently on adipocyte. Taken together, these results provide an important new insight into SCME, indicating that it possesses anti-obesity activity through pancreatic lipase inhibition, anti-adipogenic and lipolysis effects. SCME may therefore be utilized as a promising source in the field of nutraceuticals. The identification of active compounds that confer the anti-obesity activities of SCME may be a logical next step.

Anti-Inflammatory Effects and Cytoprotective Effects of Smilacis Chinae Radix (토복령의 항염증 및 세포보호 효과에 미치는 영향)

  • Lee, Seon-Goo
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.23 no.1
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    • pp.57-62
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    • 2009
  • Smilacis Chinae Radix has been used as an anti-inflammatory agent. This study was performed to anti-inflammatory and MAP kinase signaling pathway in vitro. Experimental studies were obtained by measuring the Cytotoxicity, production of NO, PGE2, TNF-$\alpha$ and protein level of catalase, SOD, MAP kinase, The results were summarized as follows: Smilacis Chinae Radix was not cytotoxic effects against Raw264.7 and HEK293 cells. Concentration of $100{\mu}g/m{\ell}$ Smilacis Chinae Radix inhibited the production of NO in the Raw264.7 cell stimulated with LPS. All concentrations of Smilacis Chinae Radix not significantly inhibited the production of PGE2 in the Raw264.7 cell stimulated with LPS. All concentrations of Smilacis Chinae Radix did not inhibit the production of TNF-$\alpha$ in the Raw264.7 cell stimulated with LPS. Smilacis Chinae Radix has not effect of blocking NF-${\kappa}B$ into nucleus in LPS-induced macrophage Raw264.7 cell. Smilacis Chinae Radix has the effect of Cytoprotection through activation of ERK and inhibition of p38 and JNK. Accordingly the results show Smilacis Chinae Radix could induce anti-inflammation and Cytoprotection effects against In vitro, but it needs more research on the precise mechanism of such effects.

Enhanced Anti-oxidant Activity Effects of Smilax china L. Rhizome Water Extracts Added with Its Fermented Leaf Water Extracts (발효 청미래덩굴잎 추출물의 혼합에 의한 토복령의 항산화활성 증진효과)

  • Lee, Sang-Il;Lee, Ye-Kyung;Kim, Soon-Dong;Shim, Soon-Mi;Yang, Seung Hwan;Cheng, Jinhua;Suh, Joo-Won
    • Journal of Applied Biological Chemistry
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    • v.57 no.2
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    • pp.145-152
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    • 2014
  • To evaluate the improving effects of antioxidant activity, we observed antioxidant capacities such as electron donating ability (EDA), Ferric reducing antioxidant power (FRAP), inhibitory activity of xanthine oxidase (XO) and aldehyde oxidase (AO), and sensory characteristics on mixture of Smilax china L. root water extract added with water extract of fermented S. china L. leaf by Aspergillus oryzae (FSCL). Those contents of mixture with higher ratio of FSCL were proportionally high. And OD475 of mixture with higher ratio of FSCL was almost proportionally high ($R^2=0.9850$). Antioxidant capacities of EDA and FRAP of the mixture was higher than that of non-mixture. In addition, XO inhibitory activity ($IC_{50}$) of A (1.19) was 59.80% higher than that of F (2.96), and the activity of mixture by the higher ratio of FSCL was proportionally low ($R^2=0.9490$). Taste acceptability of A was slightly higher than that of F, whereas that of C was highest. And color acceptability of 40-80% mixture was higher than those of A, F, and B. Overall acceptability of C and D was highest than those of others. Moreover, hot water extract of S. china L. leaf fermented with A. oryzae was maroon color, which looks like Puerh tea style, and mixture of S. china L. root extract added with hot water extract of S. china L. leaf was high acceptability of beverage. These results suggest that mixture of extract of S. china L. root and hot water extract of S. china L. leaf fermented with A. oryzae could improve antioxidant activities.

간손상에 대한 해독제 개발 및 안전성 평가 방법에 관한 연구

  • 유은영;김상건;정기화
    • Proceedings of the Korean Society of Applied Pharmacology
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    • pp.327-327
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    • 1994
  • 본 연구에서는 간손상에 대한 해독제의 연구 및 안전성 평가연구로서 H$_2$-rcccptor길항제와 한방에서 건위역으로 이용되는 생약추출물 (생강, 정향 및 후박나무의 수피추출물, 강활, 시호, 토복령 및 금은화)이 화합물로 유발된 간독성에 미치는 효과를 고찰하였다. 또한 식물의 2차 대사산물로서 식품, 의약품 및 화장품 향신료 등의 첨가제로 이용되는 monotcrpcnoids중 $\alpha$-pinene, limonenc, gcraniol 및 cincol 이 phase II 효소계중 glutathione S-transferase(GST)의 class alpha 및 mu family계 효소와 microsomal cpoxidc hydrolase(mEH)의 발현에 미치는 영향을 관찰하였다.

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Detection of Estrogen-like Activities of Hydrothermal and Ethanol Extracts of Oriental Medicines (한약재 열수 및 에탄올 추출물의 여성호르몬 유사활성 검증)

  • Lee, Dong-Geun;Jo, Jung-Kwon;Lee, Sang-Hyeon
    • Journal of Life Science
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    • v.29 no.9
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    • pp.1023-1026
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    • 2019
  • The purpose of this study was to investigate the estrogen-like activities of eight oriental medicines, including red ginseng, fenugreek, and dandelion. Hot water and ethanol extracts were prepared from Cervus nippon temminck (Nokgaksang), Cynanchum wilfordii (Baeksuo), Lespedeza cuneata (Yagwanmun), Panax ginseng (red ginseng), Smilax china (Toryeong), Taraxacum platycarpum (Mindeulre, dandelion), Tribulus terresteis (Namgase), and Trigonella foenum-graecum (Horopa, fenugreek). Then, estrogen-like activities were verified by the in vitro transcriptional activity assay. The extracts showing estrogen-like activities were red ginseng, Baeksuo, fenugreek, Yagwanmun, and dandelion in the ethanol extracts, and red ginseng, fenugreek, and Baeksuo in the hydrothermal extracts. Red ginseng extract showed a higher activity than the standard $10^{-8}M$ $17{\beta}$-estradiol in both the hydrothermal and ethanol extract at $500{\mu}g/ml$, while the $50{\mu}g/ml$ of the red ginseng ethanol extract and the $500{\mu}g/ml$ of the Baeksuo hydrothermal extract showed estradiol activities between $10^{-9}$ and $10^{-8}M$ $17{\beta}$-estradiol. This shows that it would be possible to contribute to the development of functional materials using red ginseng, Baeksuo, fenugreek, and dandelion.

Anticariogenic and Antioxidant Activities from Medicinal Herbs (생약재의 항충치 및 항산화효과 탐색)

  • Park, Yun-Mi;Kim, Seon-Jae;Jo, Kwang-Ho;Yang, En-Jung;Jung, Soon-Teck
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.3
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    • pp.284-293
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    • 2006
  • We have tested 41 herbal medicines to search for a natural substance with antimicrobial activity against Streptococcus mutans and five types of oral bacteria. We have also investigated antioxidative activity of these herbal medicines. Antimicrobial activity against Streptococcus mutans and five types of oral bacteria was analyzed using ethanol extracts of herbal medicines. Extracts from Illicium verum and Amomum xanthioides showed 98% inhibitory activity against Sterptococcus mutans. The effect of Thuja orientalis on S-1 and Thuja orientalis and Amomum xanthioides on S-2 were 95% and 94%, respectively. Nelumbo nucifera was 94% effective on S-5. The inhibitory activities of the herbal medicines against glucosyltransferase (GTase) were determined using purified from Streptococcus mutans and five types of oral bacteria. Extract from Illicium verum and Amomum xanthioides showed 94% effectiveness on Streptococcus mutans. Amomum xanthioides showed 95% effectiveness on S-1 and Thuja orientalis showed 96% effectiveness on S-5. In antioxidant activities of the herbal medicines, extract from Thuja orientalis showed the highest level of 81.08% DPPH radical scavenging activity and Illicium velum extract also showed high antioxidative activity of 80.45%. Thuja orientalis had a large amount of phenolic compound with $115.24\;{\mu}g/mL$ among the herbal medicines.

Protective effects of Smilacis Glabrae Rhizoma on acute pancreatitis (토복령(土茯苓) 물 추출물의 급성 췌장염 보호 효과)

  • Kim, Myoung-Jin;Park, Sung-Joo
    • The Korea Journal of Herbology
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    • v.33 no.2
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    • pp.45-52
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    • 2018
  • Objectives : Smilacis Glabrae Rhizoma (SGR) has been used in traditional medicine for treating diseases related to inflammation and cancer. However, the protective effect of SGR has not been reported in the study of acute pancreatitis (AP). Therefore, this study was performed to evaluate the protective effects of SGR water extract on cerulein-induced AP. Methods : AP was induced by intraperitoneal injection of supramaximal concentrations of stable cholecystokinin analogue cerulein ($50{\mu}g/kg$) 6 times at intervals of one hour in mice. SGR water extract (0.01, 0.05, or 0.1 g/kg) was administrated intraperitoneally 1 h prior to the first injection of cerulein. The mice were sacrificed at 6 h after the final injection of cerulein. Pancreas and lung were rapidly removed for histochemical examination and myeloperoxidase (MPO) assay. In addition, polymerase chain reaction (PCR) was performed to examine mRNA levels of proinflammatory cytokines such as interleukin(IL)-6, $IL-1{\beta}$ and Tumor necrosis $factor(TNF)-{\alpha}$. Results : Administration of SGR water extract significantly inhibited pancreatic weight to body weight ratio. In addition, SGR treatment inhibited the histological damages and increase of MPO activity in both pancreas and lung during AP. Also, mRNA levels of IL-6 but not $IL-1{\beta}$and $TNF-{\alpha}$ were inhibited by SGR water extract against AP. Conclusion : Our results revealed that pre-treatment of SGR water extract reduces the severity of cerulein-induced AP. Therefore, this study suggest that SGR could be used as a drug or agent to prevent AP.