• Title, Summary, Keyword: 컬럼관

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A Study on Capillary Column Ion Chromatography

  • 김호현;표동진
    • Proceedings of the Korean Environmental Sciences Society Conference
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    • pp.115-116
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    • 2001
  • 본 연구에서는 이온 크로마토그래피로 환경오염물질들을 분석하고자 할때의 단전들을 해소하기 위하여 모세관 컬럼 이온 크로마토그래피를 개발하였다. 모세관 컬럼은 두가지 형태로 개발하였는데 하나는 packed capillary 컬럼이며 다른하나는 open tubular capillary 컬럼이다. 또한 위와 같이 모세관 컬럼을 개발하여 사용하게됨을써, 부수적으로 펌프, 시료주입기, 억압장치 그리고 전도도 셀 등을 모두 적은 부피를 다룰 수 있는 구조로 바꾸어 주었다. 따라서 본 연구의 결과로 미량환경오염 물질의 분석에 모세관 컬럼 이온크로마토그래피를 이용할 수 있는 가능성을 확인할 수 있었고, 나아가 이온 크로마토그래피의 소형화로 현장에서 직접 분리, 분석을 할 수 있으리라 기대된다.

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Development of Capillary Columns for Ion Chromatography (이온 크로마토그래피용 모세관 컬럼의 개발)

  • Kim, Ho-Hyun;Pyo, Dong-Jin
    • Analytical Science and Technology
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    • v.12 no.6
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    • pp.521-527
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    • 1999
  • In this study, capillary columns for ion chromatography were developed to analyze trace amount of ions in samples. When small I.D. capillary columns are used in ion chromatography, the typical flow rate of the mobile phase is $5{\sim}15{\mu}L/min$ and the typical column length is 50~150 mm. The capillary columns were made using RSL-300 fused silica capillary(I.D.: 0.53 mm, O.D.: 0.67 mm) and AG14 column resin(support : polystyrene-divinylbenzene, functional group : alkyl quaternary ammonium). The new conductivity cell and suppressor were also developed and made for capillary column ion chromatography. When several anions (fluoride, nitrite, nitrate, chlorate) were analyzed using these capillary columns, reproducible and good chromatograms were obtained.

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Development of Open Tubular Capillary Columns for Ion Chromatography (이온 크로마토그래피용 Open Tubular Capillary 컬럼의 개발)

  • Pyo, Dong Jin;Kim, Ho Hyun
    • Journal of the Korean Chemical Society
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    • v.45 no.2
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    • pp.143-148
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    • 2001
  • In this study, open tubular capillary columns for ion charomatography were developed to analyze trace amount of ions in samples. When small I,D. capillary column length is 1.0~5.0 m. The capillary columns were made using fused silica capillary(I.D:50㎛) and DMEOHA latex particles. The new conductivity cell and suppressor were also developed and made for capillary column ion chromatography. When several anions(fluoride, nitrite, nitale,chlorate,phosphte, sulfate) were analyzed using these capillary columns. reproducible and good chromatograms were obtained.

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Preparation of Microcolumns Made of Glass-Lined Stainless Steel Tubing or Other Materials and Their Chromatographic Application (내벽이 유리로 코팅된 스테인레스 스틸 관 및 기타 재질을 이용한 마이크로 컬럼의 제작과 그의 크로마토그래피 응용에 관한 연구 (Ⅱ))

  • An, Hyun Joo;Cheong, Won Jo
    • Journal of the Korean Chemical Society
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    • v.39 no.11
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    • pp.863-868
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    • 1995
  • We have prepared microcolumns made of stainless steel tubing, glass-lined stainless steel tubing, and silica-lined stainless steel tubing and examined their performances comparatively. The length of columns was fixed as 30 cm, and the I.D.'s, either 1 mm or 0.5 mm. Octadecyl bonded silica paticles $(5\mu)$ were used as the stationary phases. p-Nitroaniline, N,N-dimethyl-o-nitro-p-toluidine,and ethylbenzene were used as the test solutes. The glass-lined or silica-lined stainless steel columns showed better performances than the plain stainless steel column did. The number of theoretical plates over 10,000 were obtained even for the 0.5 mm I.D. (30 cm long) column as long as the glass-lined stainless steel tubing was used.

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Development of Conductivity Cell and Suppressor for Capillary Column Ion Chromatography (모세관 컬럼 이온 크로마토그래피를 위한 Conductivity Cell과 Suppressor의 개발)

  • Pyo, Dongjin;Kim, Hohyun
    • Analytical Science and Technology
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    • v.12 no.2
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    • pp.89-93
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    • 1999
  • In this study, conductivity cell and suppressor for micro-column ion chromatography were developed to analyze ions in small columns of samples. With a capillary column, the flow rate of the mobile phase is so small (usually $5{\sim}20{\mu}L/min$) that the usual conductivity cell can not be used. Therefore, we developed a new type of conductivity cell and suppressor which have small inner volumes. The conductivity cell was made with two Pt hypodermic needles (i.d. 0.010 mm) which are slightly separated (about $2{\mu}m$), and the suppressor was made of Nafion tubings. When several anions(fluoride, nitrite, nitrate, chlorate) were analyzed using developed conductivity cell and suppressor, a good chromatogram was obtained.

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Studies on Analysis of Sterols in Mayonnaise by GLC with Packed and Capillary Column (충전컬럼 및 모세관컬럼 가스크로마토그라프에 의한 마요네즈 중의 스테롤 분석연구)

  • Kim, Hyean-Wee;Jeong, So-Young;Jeong, Chang-Ki;Yoon, Hyeong-Sik;Park, Ki-Moon;Ahn, Peong-Ug;Choi, Chun-Un
    • Korean Journal of Food Science and Technology
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    • v.24 no.1
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    • pp.82-85
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    • 1992
  • Sterols in mayonnaise were determined by the gas liquid chromatographic(GLC) analysis using two different columns, packed column(silicone SE30) and fused silica capillary column(CBPl). Four kinds of sterol(cholesterol, campesterol, stigmasterol and ${\beta}-sitosterol$) were detected in mayonnaise. The method using capillary column proved to be superior in rapidity, reproducerbility and separability for the determination of sterols. The ranges of concentration of egg york in mayonnaise predicted from cholesterol content using packed and capillary columns were $2.69{\sim}7.11%\;and\;2.42{\sim}6.08%$, respectively. Analyzing the composition of campesterol, stigmasterol and (${\beta}-sitosterol$ in mayonnaise, it could be known that soybean oil and cottonseed oil are commonly used for the manufacture of mayonnaise.

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Development of Portable Gas Chromatography / Photoionization Detector System (휴대용 기체 크로마토그래피 / 광이온화 검출기 시스템의 개발)

  • Kim, Man Gu;Sim, Ji Hui;Lee, Dong Su;Lee, Yong Geun
    • Journal of the Korean Chemical Society
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    • v.38 no.2
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    • pp.151-159
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    • 1994
  • The portable gas chromatography system was developed which was consisted of ambient vapor sampler(AVS), short capillary column(3 m long, 0.32 mm i.d. GC(SCCGC), photoionization detector (PID) and vacuum pump which was operated at subambient pressure. The seletion of capillary column was based on the theoretical calculation from Golay equation. The pressure ratio of column inlet and outlet appropriated between 1.03 and 1.2 in the system. The available column flow were 0.87∼4.63 ml/min at the pressure ratios. The AVS consisted of three concentric tubes and enables rapid, repetitive introduction of vapor samples directly into capillary column and showed good reproducibility. The subambient column outlet pressure with PID resulted in a significant increase in the optimum column flow, permitting rapid analysis. The baseline separation of m-xylene and o-xylene was able to within 40 second with the system. Parameters affecting the column resolving power were sampling duration, column length and diameter, and the pressure ratio. Effects of these parameters were investigated using bezene derivative compounds.

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A Study on the Separation and Determination of Organochlorine Pesticides by Capillary Column Gas-liquid Chromatography (모세관 컬럼 기체크로마토그래피에 의한 유기염소제 농약의 분리 정량에 관한 연구)

  • Taek-Jae Kim;Yun-Woo Eo;Young-Sang Kim
    • Journal of the Korean Chemical Society
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    • v.31 no.5
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    • pp.425-433
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    • 1987
  • The separation of 19 organochlorine pesticides was studied by capillary column gas chromatography. The applicability of this method to the fresh rice was investigated. Comparison of the resolution of pesticides by OV-17 and SE-30 capillary columns revealed that OV-17 column (25m ${\times}$ 0.20mm, 0.27${\mu}$m thickness) showed better resolution. The relative standard deviations (RSD) for their retention times and peak areas obtained by splitless injection mode were within 0.16% and 8.2%, respectively. The recoveries of pesticides spiked in fresh rice were mostly over 85% and their RSDS did not exceed 11.4%.

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Studies on Simultaneous Analysis of Organophosphorus Pesticide Residues in Crops by Gas-Liquid Chromatography (II). Separation of Pesticides by Capillary Column (기체-액체 크로마토그래피에 의한 농작물 중 유기인제 잔류농약의 동시 분석에 관한 연구 (제 2 보). 모세관 컬럼에 의한 분리)

  • Kim, Taek-Jae;Eo, Yun-Woo;Kim, Young-Sang
    • Journal of the Korean Chemical Society
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    • v.31 no.3
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    • pp.236-243
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    • 1987
  • The optimum resolution condition of 11 organophosphorus pesticides by gas-liquid chromatography was studied using a capillary column. The injection modes and types of vaporization tubes were evaluated in relation to pesticide analysis. The best reproducibility of peak heights of pesticides was obtained by the split mode with the vaporization tube packed with quartz wool. A 25m SE-30 capillary column provided better resolution than SE-54 column for their optimum resolution. The column at $200^{\circ}C$ initially was heated by $5^{\circ}C/min\;to\;230^{\circ}C$ followed by $10^{\circ}C/min\;to\;270^{\circ}C$ and the temperatures were held constant for 5 minutes at $230^{\circ}C\;and\;270^{\circ}C$, respectively. The relative standard deviations of their retention times were less than 0.19%.

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