A study was conducted to investigate the behavior of pollutant forms at each area in the sewage treatment plant by natural purification method. The sewage treatment plant by natural purification method that consisted of aerobic and anaerobic area was constructed. The efficiency of sewage treatment depending on the operation time, the loading amount of pollutant season variation were investigated. The removal amount of BOD, COD, TOC and SS in the aerobic area was significantly increased as the treatment proceeded. Decreased BOD, COD, TOC, SS, T-N and T-P depending on the loading amount of pollutants in the aerobic and ananerobic area were mostly insoluble BOD (IBOD), insoluble COD (ICOD), soluble TOC (STOC), volatile SS (VSS), dissolved T-N (DTN) and dissolved T-N (DTP) types, respectively. The removal efficiency of BOD, COD, TOC, SS, T-N and T-P in hot season (summer and autumn) were more than that in cold season (spring and winter). The removal efficiencies of BOD, COD, TOC, SS, T-N and T-P of the effluent were about 92, 89, 73, 95, 46 and 84% in all seasons, respectively.