E-learning in Korea supports different educational needs of diverse learners. E-learning became one of the major source of educational services for schools, higher education, lifelong learning, and for special education. Many e-learning contents offered by cyber universities use HTML, CSS, and Flash, and these are known to have limitations on accessibilities. People with disabilities or aged have problems accessing such contents. The purpose of this study is to evaluate accessibility of Flash e-learning contents offered by 9 cyber universities. AccChecker is used to assess accessibility of the contents. The result shows many errors and warning with Text Equivalents, Keyboard Navigation, Properties, Depth of Tree, and Structures that restrict access. In order to improve the quality and expansion of quality e-learning contents, we need aggressive measures to obtain accessibility of contents, and these should be designed at the planning phase rather than adjusted during the development stage. Furthermore, it is vital to train instructional designers, developers and the CEOs to realize the importance of accessibility and learn appropriate skills to increase accessibilities of e-learning contents.