• Title, Summary, Keyword: 이수성

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Effect of Cytosine Arabinoside, 3-Aminobenzamide and Hydroxyurea on the frequencies of radiation-induced micronuclei and aneuploidy in human lymphocytes (DNA 회복 저해제 Cytosine Arabinoside, 3-Aminobenzamide 및 Hydroxyurea가 방사선에 의해 유도된 소핵과 이수성에 미치는 영향)

  • Cho, Yoon-Hee;Kim, Yang-Jee;Kang, Chang-Mo;Ha, Sung-Whan;Chung, Hai-Won
    • Journal of Radiation Protection and Research
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    • v.30 no.4
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    • pp.209-219
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    • 2005
  • This study was carried out to examine the effect of the DNA repair inhibitors, Cytosine Arabinoside(Ara C), 3-Aminobenzamide(3AB) and Hydroxyurea(HU) on the frequencies of radiation-induced micronuclei(MNi) and aneuploidy. Irradiated lymphocytes(1-3Gy) were treated with DNA repair inhibitors, Ara C, 3AB and HU for 3 hours and CBMN assay - FISH technique with DNA probe for chromosome 1 and 4 was performed. The frequencies of x-ray induced MNi and aneuploidy of chromosome 1 and 4 were increased in a dose-dependent manner. Ara C, 3AB and HU enhanced the frequencies of radiation-induced MNi and the frequencies of radiation-induced aneuploidy of chromosome 1 and 4 were enhanced by HU and Ara C while no effect was observed by 3AB. The frequency of radiation-induced aneuploidy of chromosome 1 was higher than that of chromosome 4. These results suggest that there are different mechanisms involved in the formation of MNi and aneuploidy by radiation.

Development of Water Policy Indicators : Water Use Indicators (이수분야 수자원 정책지표의 개발)

  • Choi, Dong-Jin;Park, Doo-Ho;Park, Seong-Je;Lee, Joo-Hyeon;Lee, Hyo-Jung
    • Proceedings of the Korea Water Resources Association Conference
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    • pp.371-375
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    • 2009
  • 국내에서는 수자원 관리 지표에 관한 연구가 부분적으로 이루어지고 있으나, 지표를 통하여 정책목표를 설정하고 정책의 성과를 평가하는 데까지 이르지 못하고 있다. 본 연구는 우리나라의 이수분야 수자원 정책을 종합적이고 객관적으로 평가할 수 있는 지표를 개발하고 이를 국가단위, 유역단위, 지역단위에서 적용하기 위한 것이다. 이수 분야의 핵심지표를 선정하기 위해서 이수분야의 정책목표를 기준으로 4가지로 구분하고, 이를 정량적으로 제시하고 평가할 수 있는 지표에 대해서 검토하였다. 이수분야의 4가지 영역은 안정성, 효율성, 형평성과 지속가능성으로 구분하였다. 안정성은 강수, 기후변화, 인구, 경제, 사회 등 장래 환경이 불확실한 상황에서도 안정적으로 물 부족에 대응할 수 있는 능력이 확보되는 것을 말한다. 효율성은 물의 사용량과 누수율을 줄이고, 생산성과 재이용률을 제고하며, 물관리의 건전한 재정을 확보하기 위해서 비용회수체계를 개선하는 것 등을 포함한다. 형평성은 도시와 농촌, 지역간 물이용의 격차, 요금의 격차, 지역간 물자급률의 격차 등을 개선하여 사회적 약자나 소외계층의 물이용 능력을 높이는 것을 주요한 내용으로 한다. 지속가능성은 하천환경의 기능을 훼손하지 않는 범위 내에서 수자원을 이용하고, 환경 개선, 생태계 보존, 친수활동 등에 대한 물수요에 대응하는 것이다. 최종적으로 선정된 이수분야의 지표는 가뭄시 물부족량, 물부족 지역 비율, 국가 물자급률, 하천취수율, 환경용량 확보율, 물이용 공평성, 물재정 건전성 등 7개였다. 각 지표별로 지표의 정의와 산정방법 등을 제시하고, 지표를 통하여 이수분야의 수자원 정책을 평가하였다. 평가는 먼저, 국가간 비교를 하였고, 다음으로 국내의 유역간 비교, 지역간 비교의 순으로 진행하였다. 평가 결과 우리나라의 국가 물자급률과 취수율이 보통수준 이하로 매우 취약하고, 물이용의 공평성과 물관리 재정 건전성은 보통보다는 높은 수준으로 평가되었다. 평가 결과를 통해서 중장기 수자원 관리 정책의 목표를 정량적으로 제시하고, 분야별 정책 추진방향을 제안하였다.

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Analyses of Macrozoobenthic Communities at the Inter - and Subtidal Zones of Tokdong , Charando and Isudo Islands along Southern Coast of Korea (남해안 덕동 및 이수도와 자란도 지역의 조간대 및 아조대 저서동물군집 분석)

  • 최병래;박중기;이종락
    • The Korean Journal of Malacology
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    • v.11 no.1
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    • pp.35-50
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    • 1995
  • 저자들은 1994년 7월부터 1995년 2월까지 남해안 마산만의 덕동 지역과 대주군인 자란도 및 이수도에서 오염원의 영향을 민감하게 받는 종을 선별하기 위하여 조간대 및 아조대의 저서동물 군집을 채집하고 정성, 정량적 분석을 실시하였다. 조사결과 덕동 지역 군집은 대조군인 자란도나 이수도에 비하여 구성 종 수가 현저히 적었고 다양도지수 및 균등도가 낮았으며 그 다음은 자란도와 이수도순이었다. 우점도는 덕동이 가장 높았고 자란도, 이수도순이었다. 또한 조사지역 내에서 연체류중 패각만 채집된 종수가 덕동에서 19종, 자란도 3종, 이수도 4종으로 중복된 것을 제외하면 총 25종이 되며 이들 또한 오염과 관계 있을 것으로 예견된다.

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Analysis of radiation-induced micronuclei and aneuploidy involving chromosome 1 and 4 by FISH technique (FISH 기법을 이용한 방사선에 의한 소핵과 이수성 분석)

  • Chung, Hai-Won;Kim, Tae-Yon;Cho, Yoon-Hee;Kim, Su-Young;Kang, Chang-Mo;Ha, Sung-Whan
    • Journal of Radiation Protection and Research
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    • v.29 no.4
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    • pp.243-249
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    • 2004
  • The cytokinesis-block micronucleus (CBMN) assay in combination with FISH technique using chromosome-specific centromeric probes for chromosome 1 and 4 was performed in mitogen stimulated human lymphocytes which were exposed to x-radiation to identify different sensitivity of chromosomes to the induction of micronuclei(MN) and aneuploidy by radiation. The frequencies of micronucleated cytokinesis-blocked(MNCB) cells and MN in binucleated lymphocytes(BN) increased with the increase in radiation dose. A significant induction of aneuploidy of chromosome 1 and 4 were found. The frequency of aneuploidy of chromosome 1 and 4 in the control were 9 per 2,000 BN cells and this increased to 47 and 71 following irradiation at a dose of 1 and 2 Gy, respectively. The induction of aneuploidy of chromosome 1 was higher than that of chromosome 4. The frequency of aneuploid BN cells with MN exhibiting positive centromere signal for either chromosome 1 and/or 4 increased in a dose dependent manner, and that for chromosome 1 is higher than that for chromosome 4. Among the total induced MN in irradiated lymphocytes, smaller proportion of MN exhibit centromeric signal of chromosome indicating that radiation-induced MN are mainly originated from chromosomal breakage rather than chromosomal non-disjunction. These results suggest that x-radiation can induce aneuploidy and supports the finding that chromosome vary in their sensitivity to aneuploidy induction by x-irradiation.

Preimplantation Genetic Diagnosis for Aneuploidy Screening in Patients with Poor Reproductive Outcome (염색체 이수성과 관련된 비정상적 임신이 예상되는 환자에서 착상전 유전진단의 결과)

  • Kim, Jin Yeong;Lim, Chun Kyu;Cha, Sun Hwa;Park, Soo Hyun;Yang, Kwang Moon;Song, In Ok;Jun, Jin Hyun;Park, So Yeon;Koong, Mi Kyoung;Kang, Inn Soo
    • Clinical and Experimental Reproductive Medicine
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    • v.33 no.3
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    • pp.179-187
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    • 2006
  • Objectives: The risk of aneuploidies of embryos increases in advanced maternal age or parental karyotype abnormality and it results in poor reproductive outcomes such as recurrent spontaneous abortion (RSA) or repeated implantation failure (RIF). Preimplantation genetic diagnosis for aneuploidy screening (PGD-AS) can be applied for better ART outcome by selecting chromosomally normal embryos. The aim of this study is to evaluate the clinical outcome of PGD-AS and which group can get much benefit from PGD-AS among the patients expected to have poor reproductive outcome. Methods: In 42 patients, 77 PGD cycles were performed for aneuploidy screening. Patients were allocated to 3 groups according to the indication of PGD-AS: group I-patients with old age (${\geq}37$) and RIF more than 3 times (n=11, mean age=42.2 yrs.), group II-patients with RSA (${\geq}3$ times) associated with aneuploid pregnancy (n=19, mean age=38.9 yrs.), group III-parental sex chromosome abnormality or mosaicism (n=18, mean age=29.6 yrs.) including Turner syndrome, Klinefelter syndrome and 47, XYY. PGD was performed by using FISH for chromosome 13, 16, 18, 21, X and Y in group I and II, and chromosome X, Y and 18 (or 17) in group III. Results: Blastomere biopsy was successful in 530 embryos and FISH efficiency was 92.3%. The proportions of transferable embryos in each group were $32.5{\pm}17.5%$, $23.0{\pm}21.7%$ and $52.6{\pm}29.2%$ (mean ${\pm}$ SD), respectively, showing higher normal rate in group III (group II vs. III, p<0.05). The numbers of transferred embryos in each group were $3.9{\pm}1.5$, $1.9{\pm}1.1$ and $3.1{\pm}1.4$ (mean ${\pm}$ SD), respectively. The clinical pregnancy rates per transfer was 0%, 30.0% and 20.0%, and it was significantly higher in group II (group I vs. group II, p<0.05). The overall pregnancy rate per transfer was 19.6% (10/51) and the spontaneous abortion rate was 20% (2/10) of which karyotypes were euploid. Nine healthy babies (one twin pregnancy) were born with normal karyotype confirmed on amniocentesis. Conclusion: Our data suggests that PGD-AS provides advantages in patients with RSA associated with aneuploidy or sex chromosome abnormality, decreasing abortion rate and increasing ongoing pregnancy rate. It is not likely to be beneficial in RIF group due to other detrimental factors involved in implantation.

Effect of a Frontal Impermeable Layer on the Excess Slurry Pressure during the Shield Tunnelling (전방 차수층이 쉴드터널 초과 이수압에 미치는 영향)

  • Lee, Yong-Jun;Lee, Sang-Duk
    • Proceedings of the KSR Conference
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    • pp.1199-1213
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    • 2011
  • Slurry type shield would be very effective for the tunnelling in a sandy ground, but low slurry pressure could cause a tunnel face failure or a ground settlement in front of the tunnel face. Thus, the stability of tunnel face could be maintained by applying an excess slurry pressure that is larger than the active earth pressure. However, the slurry pressure should increase properly because an excessively high slurry pressure could cause the slurry flow out or the passive failure of the frontal ground. It is possible to apply the high slurry pressure without passive failure if a horizontal impermeable layer is located in the ground in front of the tunnel face, but its location, size, and effects are not clearly known yet. In this research, two-dimensional model tests were carried out in order to find out the effect of a horizontal impermeable layer for the slurry shield tunnelling in a saturated sandy ground. As results, larger slurry pressure could be applied to increase the stability of the tunnel face when the impermeable layer was located in the ground above the crown in front of the tunnel face. The most effective length of the impermeable grouting layer was 1.0~1.5D, and the location was 1.0D above the crown level. The safety factor could be suggested as the ratio of the maximum slurry pressure to the active earth pressure at the tunnel face. It could also be suggested that the slurry pressure in the magnitude of 3.5~4.0 times larger than the active earth pressure at the initial tunnel face could be applied if the impermeable layer was constructed at the optimal location.

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The Effect of Extremely Low Frequency Electromagnetic Fields on the Chromosomal Instability in Bleomycin Treated Fibroblast Cells (Bleomycin이 처리된 사람 섬유아세포에서 극저주파 전자기장의 효과)

  • Cho, Yoon-Hee;Kim, Yang-Jee;Lee, Joong-Won;Kim, Gye-Eun;Chung, Hai-Won
    • Journal of Radiation Protection and Research
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    • v.33 no.4
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    • pp.161-166
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    • 2008
  • In order to determine the effect of extremely low frequency electromagnetic fields (ELF-EMF) on the frequency of micronuclei (MN), aneuploidy and chromosomal rearrangement induced by bleomycin (BLM) in human fibroblast cells, a 60 Hz ELF-EMF of 0.8 mT field strength was applied either alone or with ELM throughout the culture period and a micronucleus-centromere assay was performed. Our results indicate that the frequencies of MN, aneuploidy and chromosomal rearrangement induced by ELM increased in a dose-dependent manner. The exposure of cells to 0.8 mT ELF-EMF followed by ELM exposure for 3 hours led to significant increases in the frequencies of MN and aneuploidy compared to BLM treatment for 3 hours alone (p<0.05), but no significant difference was observed between field exposed and sham exposed control cells. The obtained results suggest that low density ELF-EMF could act as an enhancer of the initiation process of BLM rather than as an initiator of mutagenic effects in human fibroblast.