• Title, Summary, Keyword: 유전형

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Evaluation of Human Papillomavirus Genotyping from Formalin-fixed Paraffin-embedded Specimens in Cervical Cancers (자궁경부암 파라핀 조직에서 인유두종바이러스 유전형 검사의 유용성 평가)

  • Jin, Hyunwoo
    • Journal of Life Science
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    • v.24 no.9
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    • pp.1025-1029
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    • 2014
  • Cervical carcinoma is the second leading cause of cancer-related deaths in women around the world, and it is associated with the Human Papillomavirus (HPV) infection. HPV genotyping is important for vaccine policy, etiology, natural history, and epidemiology studies. The use of formalin-fixed paraffin-embedded (FFPE) tissues for HPV genotyping by reverse blot hybridization assays (REBA) has not been clearly confirmed in retrospective studies. The aim of this study was to evaluate the usefulness and efficiency of FFPE tissues from cervical cancers for HPV genotyping. HPV genotypes were detected in 52 FFPE tissues from cervical carcinoma specimens by REBA. HPV was detected in 32 (61.5%) of 52 specimens from FFPE, among which 27 (84.4%) harbored single infections and 5(15.6%) contained multiple infections. The HPV single infections (27) were analyzed by high-risk type 18(8), 58(6), 16(5), 33(1), 35(1), 39(1), 56(1) and low risk type 11(2), 6(1), 70(1). The HPV multiple infections (5) included 16/18(2), 18/52(1), 16/56(1), 16/18/33(1). Please consider being more specific here. Do you mean the analysis? Please clarify what you mean by "included."Through this study, it has been determined that the FFPE specimen is feasible and can be used in HPV genotyping, as well as in retrospective studies.

Design of a Cylindrical Dielectric Resonator Antenna with a Dielectric Clad (유전체 클래드를 갖는 원통형 유전체 공진 안테나 설계)

  • 이권익;김흥수
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.40 no.4
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    • pp.54-59
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    • 2003
  • In this paper, a cylindrical dielectric antenna with dielectric clad is designed and analyzed. Design parameters of a cylindrical dielectric resonator antenna are calculated from the wave equation of cylindrical dielectric. The variations of characteristics of the antenna are analyzed as varying the thickness and the relative permittivity of its clad. From the results, when the ratio of the outside radius of the dielectric clad to the radius of the cylindrical dielectric is 1.3 and the relative permittivity of the dielectric clad is one-third of the cylindrical dielectric resonator antenna, the relative bandwidth of the antenna is 49%, which is improved by 2.3 times than the cylindrical dielectric resonator antennas. However, the thickness and the relative permittivity of the dielectric clad have not effect on the radiation pattern, beamwidth and gain of the antenna.

한국인 윌슨병의 임상상과 유전자형

  • Lee, Beom-Hui;Kim, Gu-Hwan;Kim, Ju-Hyeon;Jeong, Chang-U;Lee, Jin;Choe, Jin-Ho;Yu, Han-Uk
    • Journal of The Korean Society of Inherited Metabolic disease
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    • v.11 no.1
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    • pp.84-87
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    • 2011
  • 윌슨병은 ATP7B의 결함에 의해 발생하는 질환으로 구리 대사 이상질환이다. 구리대사의 이상은 체내에 구리의 축적을 유도하며, 이에 따르는 산화스트레스, 염증반응, 세포소멸사의 환경 조성, 마이토콘드리아의 손상 등이 동반되는 것으로 알려져 있다. 윌슨병은 간질환으로 발현하거나, 신경학적 증상으로 발현하는 환자도 있으며, 일부 환자는 무증상적 시기에 발현하기도 한다. 우리나라의 윌슨병 237 가계를 대상으로 한 연구에서 발현 표현형에 따른 임상상의 차이를 발견할 수 있었다. 또한, 이러한 표현형의 차이는 ATP7B의 유전형과도 일부 상관관계를 보이고 있었는데, 돌연변이의 종류나 위치에 따른 발현 임상상의 차이를 확인할 수 있었다. 그러나, 이외의 부위나 한국인에서 흔한 돌연변이에 따른 표현형은 상관관계를 밝힐 수 없었다. 향후 표현형의 다양성에 영향을 주는 요소를 찾아내고 이들이 임상경과에 미치는 영향을 밝히기 위한 노력이 필요하다.

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Selection of Peptide Vaccine Candidates against Japanese Encephalitis Virus: Approach Using Bioinformatics Database (일본 뇌염 바이러스에 대한 펩타이드 백신 후보군 도출: 생물정보학 데이터베이스를 활용한 접근법)

  • Park, Suji;Eom, Hyoji;Choi, Jae-Won;Kim, Hak Yong
    • Proceedings of the Korea Contents Association Conference
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    • pp.347-348
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    • 2018
  • 일본 뇌염 바이러스(Japanese encephalitis virus)는 작은빨간집모기(Culex spp.)를 매개로 사람에게 감염될 수 있으며, 인체에 치명적인 질병을 유발한다. 일본 뇌염 바이러스의 혈청형(serotype)은 1종류이지만, 유전형(genotype)은 5종류(GI, GII, GIII, GIV, GV)로 분류되고 있다. 현재 일본 뇌염 바이러스 백신은 아시아 지역에서 감염 빈도가 높은 유전형 3(GIII)에 대한 백신이며, 사백신(inactivated vaccine)과 약독화 백신(attenuated vaccine)이 주로 사용되고 있다. 본 연구에서는 기존 백신의 부작용을 줄이고 한계점을 개선하기 위하여, 생물정보학 데이터베이스를 활용한 접근법을 통해 펩타이드 백신 후보군을 선별하였다. 5가지의 유전형 중에서도 감염 빈도가 가장 높은 유전형 3(GIII) 및 최근 감염빈도가 서서히 늘어나고 있어 주의가 요구되고 있는 유전형 1(GI)을 연구 대상으로 선정하였다. 여러 종류의 생물정보학 데이터베이스를 활용하여 백신으로 활용가치가 높은 것으로 보고되고 있는 외피 단백질(envelope protein)에 대한 아미노산 상동성을 분석하고, 이를 바탕으로 공통 적용이 가능한 동시에 면역원성이 높은 펩타이드 3종을 백신 후보군으로 선별하였다. 더 나아가 이들의 3차원 구조 모델링을 통해 보다 백신으로 활용 가능성이 높은 펩타이드를 최종 도출하였다.

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Phenotype-genotype correlations and the efficacy of growth hormone treatment in Korean children with Prader-Willi syndrome (프래더 윌리 증후군의 유전학적 발병 기전에 따른 표현형 및 성장 호르몬 치료 효과에 관한 연구)

  • Bae, Keun Wook;Ko, Jung Min;Yoo, Han Wook
    • Clinical and Experimental Pediatrics
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    • v.51 no.3
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    • pp.315-322
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    • 2008
  • Purpose : Prader-Willi syndrome (PWS) is a complex genetic disorder, caused by the deletion of the paternally derived 15q11-13 region or the maternal uniparental disomy of chromosome 15 (mUPD(15)). In this study, we compared phenotypic differences between those patients whose disease was caused by microdeletion and those caused by mUPD(15). In addition, a comparison of the efficacy of growth hormone (GH) therapy between these two PWS genotypes was analyzed. Methods : Fifty-three patients were diagnosed as having PWS based on molecular and cytogenetic analyses and clinical features. Data that included maternal age, birth weight, a feeding problem in the neonatal period, cryptorchidism, developmental delay or mental retardation, short stature, hypopigmentation, changes in height, weight, and body mass indexes (BMI) before and after GH treatment were obtained by a retrospective review of medical records. The data from the patients with microdeletion were compared with those from the patients with mUPD(15). Results : Of the 53 patients with genetically confirmed PWS, 39 cases had microdeletion and 14 mUPD(15). Maternal ages were significantly higher in the mUPD(15) group, and hypopigmentation and a feeding problem in the neonatal period were more frequent in the microdeletion group. Growth hormone was administered to 20 patients [14 with microdeletion, 6 with mUPD(15)]. There were no differences between the two groups in height velocity, weight and height SDS, and BMI after GH therapy. Conclusion : Phenotype and genotype correlations were observed in Korean PWS patients, such as more advanced maternal ages in the mUPD(15) group and more feeding problems and hypopigmentations in the microdeletion group. Further long-term prospective studies are needed to correlate other aspects of the phenotypes.

Modified Binary Particle Swarm Optimization using Genotype-Phenotype in Genetics (유전학의 유전자형-표현형을 적용한 수정된 이진 입자군집최적화)

  • Lim, Seungkyun;Lee, Sangwook
    • Proceedings of the Korea Contents Association Conference
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    • pp.43-44
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    • 2014
  • 본 논문에서는 유전알고리즘의 유전자형-표현형을 사용한 수정된 이진 입자군집최적화의 두 번째 버전을 소개한다. 첫 번째 버전의 수정된 이진 입자군집최적화는 위치 정보에 유전학의 표현형을 사용한 반면에 제안하는 버전은 유전학의 유전자형을 사용한다. 이진 정보만을 제공하는 표현형에 비해 연속 공간 전체를 탐색공간으로 제공하는 유전자형 정보를 사용하여 해 공간을 보다 넓은 공간으로 만들 수 있다. 10개의 실험 평가 함수에 실험한 결과, 두 번째 버전은 탐색 공간이 넓고 지역최적해가 많은 함수에서 우수한 결과를 보였다.

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A New Method for Imputation of Missing Genotype using Linkage Disequilibrium and Haplotype Information (결측치가 존재하는 유전형 자료에서의 연관불균형과 일배체형을 사용한 결측치 대치 방법)

  • Park Yun-Ju;Kim Young-Jin;Park Jung-Sun;Kim Kuchan;Koh Insong;Jung Ho-Youl
    • Journal of KIISE:Software and Applications
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    • v.32 no.2
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    • pp.99-107
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    • 2005
  • In this paper, wc propose a now missing imputation method for minimizing loss of information linkage disequilibrium-based and haplotype-based imputation method, which estimate missing values of the data based on the specificity of Single Nucleotide Polymorphism(SNP) genotype data. Method for imputing data is needed to minimize the loss of information caused by experimental missing data. In general, missing imputation of biological data has used major allele imputation method. but this approach is not optima]. 1'his method has high error rates of missing values estimation since the characteristics of the genotype data are not considered not take into consideration the specific structure of the data. In this paper, we show the results of the comparative evaluation of our model methods and major imputation method for the estimation of missing values.

Distribution of Human Rotavirus Genotypes in a Tertiary Hospital, Seoul, Korea During 2009-2013 (2009년부터 2013년까지 서울의 일개 대학병원에서 동정된 로타바이러스 유전형의 분포)

  • Han, Tae Hee;Park, Sang-Hun;Chung, Ju-Young;Hwang, Eung-Soo
    • Pediatric Infection and Vaccine
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    • v.22 no.2
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    • pp.81-90
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    • 2015
  • Purpose: Group A rotavirus (RV) is most common etiologic agent of acute gastroenteritis (AGE) in children worldwide. Recently, vaccination has been introduced in several countries to reduce the disease burden caused by RV infections, but continuous surveillance of RV strains is necessary to detect the emergence of potential variants induced by vaccine-immune pressure. This study aimed to investigate the changing pattern of RV genotypes in children with AGE, following the introduction of vaccination in Korea. Methods: Genotyping of RVs by RT-PCR on the basis of VP7 and VP4 gene segment sequence was carried out on 201 rotavirus-positive stool samples, from children hospitalized with AGE between August 2009 and June 2013. We have directly sequenced PCR products and analyzed the phylogenetic tree. Results: The most prevalent G genotype was G9 (33.3%), followed by G1 (22.4%), G3 (15.9%), G2 (6.0%), G4 (3.0%), G10 (1.5%), and mixed G-type (15.4%), with some nontypeable cases (2.5%). The detected P genotypes were P[4] (45.3%), P[8] (43.8%), mixed P-type (10.4 %), and P[2] (0.5%). The G9P[4] genotype was predominantly observed in hospitalized cases in Seoul in 2010/2011, however G1P[8] has been re-emerged as the predominant genotype in the following season (P =0.004). Conclusions: It seems that the periodic fluctuation in predominance of the G1, G3, and G9 strains occurred in Korea during 2009-2013, following the introduction of RV vaccination.

Vt Close Curve Analysis for Improving Address Discharge Characteristics in Open Dielectric Structure of AC PDP (플라즈마 디스플레이의 개방형 유전체 구조에서 기입방전특성을 향상시키기 위한 Vt 폐곡선 분석)

  • Cho, Byung-Gwon
    • Journal of the Institute of Electronics and Information Engineers
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    • v.51 no.1
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    • pp.179-184
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    • 2014
  • The discharge characteristics of an open dielectric structure were investigated, especially such as a firing voltage and related wall voltage, compared with conventional panel structure based on the Vt close curve measurement in AC plasma display panel. While the front panel of the conventional structure in AC plasma display panel was composed of the glass, electrodes, and dielectric, the open dielectric structure could easily produce the discharge between the scan and the sustain electrodes by erasing the dielectric layer between two electrodes. As the open dielectric structure differ from the conventional structure, various problems were produced when driving with the conventional driving waveform. Especially, due to the changes in the discharge firing characteristics of the open dielectric structure between the scan and the sustain electrodes on the front panel, the conventional reset waveform including the address waveform needed to be modified. In this study, the Vt close curves were measured to compare the discharge firing voltages on three electrodes in the conventional and open dielectric structure and based on the Vt close curve analysis, the modified driving waveform suitable for the open dielectric structure was proposed.

Genome Type Analysis of Adenovirus Serotypes 1, 2 and 5 Isolated from Children with Lower Respiratory Tract Infections in Korea (하기도 감염 환아에서 분리된 Adenovirus 1, 2, 5 혈청형의 유전체형 분석)

  • Park, Ki-Won;Choi, Eun-Hwa;Choun, Ji-Tae;Lee, Hoan-Jong;Park, Ki-Ho
    • Pediatric Infection and Vaccine
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    • v.12 no.2
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    • pp.166-177
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    • 2005
  • Purpose : The purpose of this study was to examine the molecular epidemiology and genetic variability of adenovirus(Ad) serotypes Ad1, Ad2, and Ad5 over 14 years in Korea. Methods : A total of 382 adenoviral strains isolated from the nasopharyngeal aspirates of children with lower respiratory tract infections in Seoul, Korea from November 1990 to February 2003 were serotyped by neutralization assay with type-specific antisera. Viral DNAs were extracted from infected cell lysates by the modified Hirt procedure. Genome type(GT) was determined by DNA restriction analysis with 12 restriction enzymess(BamHI, BclI, BglI, BglII, BstEII, EcoRI, HindIII, HpaI, SalI, SmaI, XbaI, and XhoI). To evaluate the genetic relatedness, pairwise comigrating restriction fragments(PCRF) analysis was performed. Results : Of 382 strains, 33 strains(9%) were Ad1, 45 strains(12%) were Ad2, and 24 strains(6%) were Ad5. Eighteen GTs(Ad1p1-Ad1p7, Ad1a, Ad1b, Ad1b1-Ad1b3, Ad1c, Ad1d, Ad1e, Ad1e1, Ad1e2, Ad1f) among Ad1, 24(Ad2p1-Ad2p11, Ad2a, Ad2a1-Ad2a6, Ad2b, Ad2c, Ad2d, Ad2e, Ad2e1-Ad2e3) among Ad2, and 10(Ad5p1, Ad5p2, Ad5a, Ad5a1-Ad5a7) among Ad5 strains were identified. One or two strains of the vast majority of GTs were isolated during the study period while a few GTs were identified sporadically with more than 2 strains. It is notable that some GTs such as Ad1p5 and Ad5a1 appeared in cluster during a short period. In analysis of genetic relatedness, the degree of PCRFs(pairwise comigrating restriction fragments) for Ad1 varied from 79 to 99%, for Ad2, 82 to 99%, and for Ad5, 85 to 99%. Conclusion : This study established the comprehensive nomenclature systems of Ad1, Ad2, and Ad5. Diverse GTs identified in this study have crucial implications in the genomic diversity and epidemiological characteristics of Ad1, Ad2, and Ad5.

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