• Title, Summary, Keyword: 원예치료

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Preparation and Characterization of Microorganism Fermentation Cellulose as Hydrogel Wound Dressing (미생물 발효 셀룰로오스를 이용한 상처 치료용 하이드로겔의 제조 및 특성)

  • Kim, Mi-Yeong;Lim, Youn-Mook;Lee, Jong-Dae;Song, Sung-Gi;Gwon, Hui-Jeong;Park, Jong-Seok;Nho, Young-Chang;Kim, Sung-Ho;Choi, Young-Hun;Lee, Sun-Yi
    • Journal of Radiation Industry
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    • v.4 no.4
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    • pp.365-372
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    • 2010
  • Irradiation has been recognized as a highly suitable tool to aid in the formation of hydrogel. The radiation process has various advantage, such as easy process control and the lack of necessity for initiators and crosslinker. In this study, the hydrogel containing the citrus fermentation gel for the wound healing were successfully synthesized. The strength of hydrogel was increased as a function of a increasing the concentration of citrus and the irradiation doses. In addition, this hydrogel have been evaluated by the cytotoxicity and animal experiment.

Artemisia argyi H.Lev. & Vaniot Inhibits Matrix metalloproteinases in the Interleukin-1β-stimulated Primary Chondrocytes and Attenuates Osteoarthritis Progression in Mice (황해쑥의 Interleukin-1β 유도 연골세포에서 Matrix metalloproteinase들의 억제효과 및 마우스에서 관절염 진행 감소 효과)

  • Park, Chan Hum;Yang, Chang Yeol;Yang, Siyoung;Yokozawa, Takako;Shin, Yu Su
    • Proceedings of the Plant Resources Society of Korea Conference
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    • pp.94-94
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    • 2019
  • 황해쑥(Artemisia argyi H.Lev. & Vaniot)은 우수한 항염증 활성을 지닌 것으로 다양하게 보고 되었다. 그러나, 대표적인 염증 질환 중 하나인 골관절염에 미치는 영향은 현재까지 알려져 있지 않다. 따라서, 본 연구에서는 염증 유발 연골 세포 및 골관절염 유발 동물 모델에 미치는 황해쑥 효과에 대해 조사하였다. 첫째, interleukin 1 beta를 투여한 관절 연골 세포에 황해쑥 물 추출물을 투여한 후 metalloporeinase (MMP) -3 및 MMP-13의 발현을 mRNA 및 단백질 수준에서 분석 하였다. 또한, 내측 반월상 연골의 불안정화에 의해 유도 된 골관절염 마우스 모델을 사용하여 황해쑥 물 추출물의 골관절염 억제 효과를 분석 하였다. 세포 실험에서, 본 황해쑥은 MMP-3와 MMP-13의 mRNA 및 단백질 발현을 유의하게 억제하였다. 또한. 황해쑥 물 추출물을 투여한 실험 동물의 관절 조직을 Safranin O 염색을 실시하여 분석한 결과 연골 하 골판 두께의 감소 및 활막 염증 개선 효과가 관찰 되었다. HPLC를 이용한 성분 분석 결과, 황해쑥 물 추출물은 항염증 및 항관절염 활성을 가진 jaceosidin과 eupatilin을 함유하는 것으로 나타났다. 본 연구결과로부터 황해쑥은 골관절염의 치료 또는 예방에 유망한 소재로 개발될 수 있음을 시사한다.

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Anticancer Effects of the Extracts of Oriental Melon (Cucumis melo L. var makuwa Makino) Seeds (참외(Cucumis melo L. var makuwa Makino) 종자 추출물의 항암 활성)

  • Kim, Jung-Hyun;Suh, Jun-Kyu;Kang, Young-Hwa
    • Korean Journal of Plant Resources
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    • v.25 no.5
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    • pp.647-651
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    • 2012
  • The objective of this study was to investigate the anticancer effects of the extracts of oriental melon seeds. Various solvent extracts of oriental melon seeds were prepared and their anticancer effects were examined using in vitro MTT and CV assays. The anticancer effects of various extracts of oriental melon seeds were also examined in five human cancer cell lines including A549, AGS, HT-29, MCF-7 and HepG2. The ethanol extract of heated oriental melon seeds showed the potent cytotoxic effects especially against mouse hepatoma cell line(Hepa1c1c7), human hepatoma cell line(HepG2) and human breast cancer cell line(MCF-7). These data suggest that oriental melon seeds can be a promising anticancer agent against human liver and breast cancer.

Activity-guided Purification of N-benzyl-N-methyldecan-1-amine from Garlic and Its Antitumor Activity against CT-26 Colorectal Carcinoma in BALB/C Mice (활성추적분리법에 의해서 순수분리한 마늘 N-benzyl-N-methyldecan-1-amine이 CT-26 세포주 이식 BALB/C mice의 항암효과)

  • Seetharaman, Rajasekar;Choi, Seong Mi;Guo, Lu;Cui, Zheng Wei;Otgonbayar, Duuriimaa;Park, Ju Ha;Kwon, Young-Seok;Kwak, Jung Ho;Kwon, Young Hee;Min, Ji Hyun;Kang, Jum Soon;Choi, Young Whan
    • Journal of Life Science
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    • v.29 no.10
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    • pp.1062-1070
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    • 2019
  • A components of garlic (Allium sativum) have anti-proliferative effects against various types of cancer. We aimed to investigate the capacity of garlic compounds to anti-tumor on a various cancer cell lines. Fractionation of garlic extract, guided by antiproliferative activity against human gastric cancer (AGS) cells, has resulted in the isolation of N-benzyl-N-methyldecan-1-amine (NBNMA). We investigated the effect of newly isolated NBNMA from garlic cloves on the inhibition of the growth of CT-26, AGS, HepG2, HCT-116, MCF7, B16F10, and Sarcoma-180 cells for in vitro and CT-26 colon carcinoma cells in vivo. NBNMA exhibited an antiproliferative effect in CT-26 cells by apoptotic cell death. NBNMA exhibited down-regulation of anti-apoptotic Bcl-2 proteins and up-regulation of apoptotic Bad protein expression in western blot analyses. In addition, NBNMA meagre activated caspase 3 and caspase 9, initiator caspases of the extrinsic and intrinsic pathways of apoptosis. NBNMA treatment at a dose of 10 mg/kg for 21 days in experimental mice implanted with tumors resulted in significant reduction of the tumor weight (43%). NBNMA exhibited both in vitro and in vivo anticancer activity. These results indicate that NBNMA has promising potential to become a novel anticancer agent from garlic cloves for the treatment of colon carcinoma cancer.

Red Ginseng Extract Improves Liver Fibrosis in Mice Treated with the Endocrine Disruptor Bisphenol A (내분비교란물질 비스페놀 A를 처리한 마우스에서 홍삼 추출물의 간 섬유화 개선)

  • Choi, Jehun;Park, Chun Geon;Seo, Kyoung Hee;Kim, Hyung Don;Yoon, Ji Hye;Ahn, Young Sup;Kim, Jin Seong
    • Korean Journal of Plant Resources
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    • v.30 no.2
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    • pp.125-132
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    • 2017
  • Bisphenol A (BPA), a known endocrine disruptor, induces toxicity in cells and in experimental animals. Ginseng extracts were evaluated to determine whether they can inhibit BPA-induced toxicity. The antioxidant activity of fresh ginseng extract (WGE), dried white ginseng extract (DGE), and dried red ginseng extract (RGE) was measured using the DPPH assay. WGE and RGE increased DPPH free radical scavenging activity. Cell viability was measured in HepG2 cells following treatment with BPA and ginseng extracts using the MTT assay. DGE and RGE increased HepG2 cell viability following treatment with $200{\mu}M$ BPA. RGE reduced levels of biochemical markers of liver damage, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) that increased in mice following treatment with BPA. In addition, the regeneration and proliferation of damaged liver cells were significantly increased in RGE-treated mice. Moreover, RGE inhibited hepatic fibrosis in the surrounding area and in the central vein of the liver microstructure. RGE also significantly inhibited BPA-induced cytotoxicity. In addition, RGE protected liver damage and regenerated liver tissues in BPA-treated animals. These results show that RGE may represent a potential candidate drug for the treatment and prevention of liver damage caused by environmental toxins.

Antioxidant and anti-inflammatory effects of phenolic rich Hylotelephium erythrostictum extracts (폴리페놀 함량이 높은 Hylotelephium erythrosticum 추출물의 항산화 및 항염 활성)

  • Kim, Sang Suk;Park, Kyung Jin;Lee, Seung Eun;Lee, Jeong Hoon;Choi, Young Hun
    • Korean Journal of Food Preservation
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    • v.24 no.6
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    • pp.842-848
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    • 2017
  • Hylotelephium erythrostictum is commonly used as a medicinal herb. In this study, H. erythrostictum leaf (HEL), branch (HEB), root (HER), and above ground (HEAG) extracts were evaluated for their antioxidant properties. The antioxidant activities were assayed by three methods based on scavenging of DPPH, ABTS and superoxide anion radical. HEAG extract showed the highest DPPH, ABTS, superoxide anion radical scavenging activities. HEAG extract also exhibited the highest phenolic content (230 mg/g gallic acid equivalent). In our research for anti-inflammatory ingredients, the extract of HEAG inhibited the generation of nitric oxide (NO) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. To test the inhibitory effects of HEAG on pro-inflammatory cytokines, we conducted ELISA assay for the measuring the generation of tumor necrosis factor $(TNF)-{\alpha}$, IL (interleukin)-$1{\beta}$, and IL(interleukin)-6 in LPS-stimulated RAW264.7 macrophage cells. In these assays, HEAG ethanol extract showed a dose-dependent decrease in the production of $TNF-{\alpha}$, $IL-1{\beta}$, and IL-6. Based on these results, extract of HEAG could be the efficient candidate for anti-inflammatory agents.

Lipolysis Effect of Daucosterol Isolated from Mulberry (Morus alba) Leaves (뽕잎으로부터 순수분리한 daucosterol의 lipolysis 효과)

  • Li, Ke;Lee, Mi Lim;Que, Lu;Li, Mae;Kang, Jum Soon;Choi, Yung Hyun;Kim, Kyung Mi;Jung, Jae-Chul;Hwang, Dae Youn;Choi, Young Whan
    • Journal of Life Science
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    • v.27 no.12
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    • pp.1500-1506
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    • 2017
  • Plants are reservoirs of naturally occurring chemical compounds and of structurally diverse bioactive molecules. The aim of this investigation was to screen for the presence of phytochemicals responsible for the lipolysis activity in mulberry (Morus alba) leaves, which are important in traditional Asian medicinal plants. Powdered mulberry leaves were extracted with hexane, ethyl acetate, and methanol. Daucosterol was isolated from the EtOAc extract of mulberry leaves, and its structure was elucidated by NMR spectral analyses. The NMR assignments for the compound were determined using $^1H$, $^{13}C$, DEPT, COSY, HSQC, and HMBC NMR spectral data. Daucosterol showed a concentration-dependent lipolysis activity that may impart medicinal properties that can be exploited by medical practitioners for the treatment of various diseases. However, further studies should be conducted to elucidate additional mechanisms of daucosterol.

Analysis on the Present Status and Characteristics of Agro-healing in Korea (한국 치유농업의 현황 및 특성 분석)

  • Gim, Gyung Mee;Moon, Jihye;Jeong, Sun Jin;Lee, Sang Mi
    • Journal of Agricultural Extension & Community Development
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    • v.20 no.4
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    • pp.909-936
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    • 2013
  • This study investigated definition of care farming in which advanced countries in agricultural industry such as Netherlands and Belgium increase their interest and investment, and established a strategy for settlement and development of care farming industry in Korea. For achieving purpose of the study, literature review was implemented to analyze policy, system, related law, workforce of advanced countries in care farming, and experts' conference which consists of 9 representatives such as professors, researchers in areas of agriculture, health, education, horticulture, animal-assisted therapy was implemented. Also survey was conducted targeting 165 people of practitioners in care farming industry. For the result, the concept of care farming in Korea was defined as 'Industry and activity which promotes psychological social physical cognitive health of every Korean people by utilizing agricultural resources(plants, livestock, rural environment, rural culture) or related activity and output', and the purpose, resources, targets of care farming was determined by the definition.

The Application of Evaluation of Social Interaction(ESI) on Local Community Base Program for Solitary Senior Citizen (독거노인을 위한 지역사회기반 프로그램에서 사회적 상호작용 평가(ESI)의 적용- 광주지역 독거노인을 중심으로 -)

  • Kim, Jung-Mi;Park, Woong-Sik;Jang, Ji-Yeon
    • The Journal of Korean society of community based occupational therapy
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    • v.1 no.2
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    • pp.1-9
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    • 2011
  • Objective : Study purpose is to inspect not only the utility of Evaluation of Social Interaction(ESI) but also the efficiency of occupational therapy measurement in program based local community of solitary senior citizen in G region. Methods : Six solitary senior citizen in G region were participating in a community-based program were a convenience sample for this study. The Occupational Therapy Intervention Process Model (OTIPM) provided the theoretical framework. The ESI provided a pre-test performance measure and also served as a guide for an five-week occupational therapy intervention program, and as a post-test to measure intervention effectiveness. Results : This study showed that the ESI was effective in guiding therapist Intervention related to social skill performance of participants during their participation in a community-based program. As a group, the difference between pre and post ESI logit measures was also significant(t=-7.362, p=0.001). Conclusion : The study provides initial considerations for the utility of the ESI to guide intervention related to social skill performance during daily occupations in Korea. It further documents the effectiveness of the ESI in measuring occupational therapy intervention, based on OTIPM, and supports occupational therapy services provided in a community based program context for those solitary senior citizen.

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The effect of Art Therapy apply to ADHD child's problem behavior and child relationship (미술치료가 ADHD 아동의 문제행동과 또래관계에 미치는 효과)

  • Shin, Ji-Hye;Kim, Gui-Bok
    • Korean Journal of Human Ecology
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    • v.18 no.2
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    • pp.341-351
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    • 2009
  • This study shows that Art therapy has positive effects on ADHD child's problem behavior and child relationship. Also we want to provide more data to education field. We work with 3 children. They are recommended by their class teacher and they are attending J elementary school which is located in K city in Chunbuk. The experiment period was 7 weeks - 2 times a week, total is 14th- and each time required was 40-60 minutes when they finish their school. Since we want to see each ADHD child's results, we use ADHD teacher evaluating measure and Child relationship measure to compare the results after the fact. The results are Art therapy has positive effects on the ADHD child's problem behavior specially concentration, excess behavior, social skill, hostile behavior and aggregation with same ages, sociality, lack of sociality, exclusive, unstable, and hypersensitiveness.