• Title, Summary, Keyword: 용매추출법

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신장 상피세포주 A498을 이용한 대황(Rheum undulatum L.)추출물의 세포독성

  • 나명석;진종언;조남철
    • Proceedings of the Korean Journal of Food and Nutrition Conference
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    • pp.55-55
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    • 2000
  • 본 연구에서는 전통적으로 담석증, 신장치료 등의 한약제로 많이 사용하는 대왕을 여러 용매를 사용하여 얻은 대왕추출물 분액에 대한 세포독성을 여부를 MTT 정량법, NR 정량법, SRB 정량법을 이용하여 조사하였다. 1. 추출 용매 methylene chloride, ethyl acetate, butanol, water로부터 얻은 대왕추출물 모두 처리농도에 따라 세포에 미치는 영향이 증가하였다. 2. Butanol을 용매로 사용하여 얻은 대왕추출물 분액이 다른 3가지 용매로부터 얻은 대왕추출물보다 세포에 미치는 영향이 크게 나타났고 water를 용매로 사용하여 얻은 추출물이 A498 세포주에 미치는 영향이 가장 낮게 나타났다. 3. Butanol을 추출 용매로 하여 얻은 대왕추출물이 A498 세포주에 미치는 영향이 가장 컸는데 그 추출물에 대한 MTT50, NR50, SRB50값은 각각 0.63mg/m1, 0.65mg/ml, 0.68mg/ml이었고, 가장 영향이 적은 water의 경우 MTT50, NR50, SRB50값은 각각 0.84mg/m1, 0.82mg/m1, 0.80mg/ml이었다. 4. 정량방법 간의 대왕추출물에 대한 반응은 MTT 정량법이 가장 민감하게 나타났다.

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Comparison of Steam Distillation with Solvent Extraction Method in Determining Organochlorine Pesticide Residues in Soil (토양중(土壤中) 유기염소계(有機鹽素系) 살충제의 용매추출법(溶媒抽出法)과 수증기증류법(水蒸氣蒸溜法)의 비교(比較))

  • Shim, Jae-Han;Suh, Young-Tack;Park, Ro-Dong
    • Korean Journal of Environmental Agriculture
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    • v.2 no.2
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    • pp.73-77
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    • 1983
  • Principle of steam distillation has been successfully applied to the routine extraction of organochlorine pesticide residues from soil samples. Recovery of 8 organochlorine insecticides was found consistent and also comparable to that of conventional solvent extraction method. Recoveries of ${\alpha}-BHC$ and heptachlor was, however, rather poor at 47 and 45% respectively. The steam distillation method offers added advantages such as economy in time and costly solvents.

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Comparison of the chlorophyll content analysis methods of micro-algal oil (미세조류의 엽록소 분석법에 따른 함량 비교)

  • Choi, Byoungyun;Kim, Deogkeun
    • 한국신재생에너지학회:학술대회논문집
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    • pp.113.1-113.1
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    • 2011
  • 미세조류 내의 엽록소는 바이오디젤 전환 반응에서 산 촉매의 활성을 억제 할 뿐만 아니라, 짙은 색상을 띄게하여 바이오디젤 품질규격으로부터 벗어나게 한다. 미세조류의 엽록소 분석은 용매에 의해 엽록소를 추출한 후, 흡광도를 측정하여 그 함량을 계산하는 방법을 널리 사용하고 있다. 건조된 미세조류의 분석은 선택되는 용매에 따라 최대 추출량이 달라지는 것을 제외하고 큰 문제가 없지만 미세조류를 lipid 오일로 변환하면, 용매에 녹지 않아 추출이 되지않는 문제가 발생하여 흡광도 측정을 어렵게 한다. 따라서 미세조류의 형태가 powder일 때와 오일인 경우를 구분하여 용매를 선택해야 하며, 오일 또는 powder 형태 구분 없이 사용할 수 있는 분석법을 적용하여 서로 다른 엽록소 함량을 비교한 후 분석법 간의 상호 장단점을 파악해야한다. 본 연구에서는 메탄올을 용매로 사용하는 분석법(porra et al.)과 아세톤을 용매로 사용하는 분석법(Humphrey and Jeffrey)을 적용하여 엽록소 함량을 비교하였고, AAS(Atomic Absorption Spectrometer)를 통한 Mg 함량 측정을 통해 엽록소 함량을 계산하는 분석법간의 차이를 확인하였다.

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Recovery of Cobalt from the Wastewater produced during Malonate Process by Solvent Extraction (말로네이트 제조 공정 폐수로부터 코발트 회수: 용매추출법 적용)

  • 문영환
    • Journal of Environmental Science International
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    • v.6 no.2
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    • pp.133-139
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    • 1997
  • The solvent extraction method was applied on the wastewater produced during malonate(malonic acid esters) process to recover cobalt. DEHPA and PC88A were used as organic solvent From separation funnel experiment(batch experiment), the effects of vari- ous parameters (pH, cobalt concentration, reaction rate, and stripping temperature) on solvent extraction were examined and these data were used to derive equilibrium curve. A mixer-settler experiment (continuous experiment) of bench scale was also carried out for the plant construction and a Mccabe-Thiele diagram was obtained. The results of these experiments indicate that cobalt is recoverable above 99 oyo and that its purity as cobalt sulfate Is higher than 99.9 wt%.

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Cytotoxicity of Crude Extracts of Rheum uudulatum L. with Human Kidney Epithelial Cell A498 (신장 상피세포주 A498을 이용한 대황(Rheum undulatum L.)추출물의 세포독성)

  • 나명석;진종언;조남철
    • The Korean Journal of Food And Nutrition
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    • v.13 no.5
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    • pp.460-464
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    • 2000
  • We have evaluated cytotoxic effects of four crude extracts of methylene chloride, ethyl acetate, butanol, water layer isolated Rheum undulatum in A498 cell line, human kidney epithelial cells. The cytotoxic evalutation was measured by colorimetric assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide(MTT) , neutral red(NR) and sulforhodamine protein B(SRB). These results obtained are as follows : MTT, NR and SRB quantities were significantly decreased in cultured A498 cells treated four crude extracts by increased concentrations. The cell cytotoxic effect of crude extracts of butanol layer was more stronger than others layer. The values of MTT$\sub$50/, NR$\sub$50/, SRB$\sub$50/ of crude extract of butanol layer and were measured both 0.63 mg/ml, 0.65 mg/ml, and 0.68 mg/ml, respectively and the values of water layer were 0.84 mg/ml, 0.82 mg/ml. and 0.80 mg/ml. respectively in cultured A498 cell line.

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Extraction of Triterpenoid Saponin (glycyrrhizin) from Liquorice by Co-solvent Modified Supercritical Carbon Dioxide (보조용매로 변형된 초임계 이산화탄소에 의한 감초의 triterpenoid saponin(glycyrrhizin)의 추출)

  • Kim, Hyun-Seok;Kim, Byung-Yong;Lim, Gio-Bin
    • Korean Journal of Food Science and Technology
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    • v.34 no.6
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    • pp.1057-1061
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    • 2002
  • Effects of modifier and soaking on extraction of triterpenoid saponin (glycyrrhizin) from liquorice were examined using supercritical $CO_2(SC-CO_2)$ at 50 MPa, $60^{\circ}C$, and flow rate of 3 mL/min, and glycyrrhizin content was analyzed by HPLC. Additon of undiluted methanol, ethanol or isopropanol as modifier to $SC-CO_2$ had little influence on extraction yield of glycyrrhizin. Soaking process using water increased the extraction yield as the sample to solvent ratio was increased. Addition of 70% methanol, ethanol or isopropanol to $SC-CO_2$ significantly increased the extraction yields, with 70% methanol resulting in the highest yield. When water at 90% (w/w) of sample weight was used for soaking, the extraction yield and rate increased, 70% ethanol-modified $SC-CO_2$ was almost equal to that obtained using 70% methanol.

Solvent Extraction Separation of Re (VI) from Hydrochloric Acid Leaching Solution of Spent Super Alloy by Alamine 304-1 (폐 초내열합금 염산침출 용액으로부터 Alamine304-1을 이용한 레늄의 용매추출분리)

  • Ahn, Jong-gwan;Jung, Hee-Kyeoung;Jang, Jae-Young;Kim, Min-Seuk
    • Journal of the Korean Institute of Resources Recycling
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    • v.24 no.5
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    • pp.56-62
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    • 2015
  • Solvent extraction experiments for the separation and recovery of Re from hydrochloric acid leaching solution of spent super alloy by Alamine 304-1 were carried out. The effects of some variables, such as the nature and concentration of the extractants, HCl concentration, and the presence of impurities were investigated. The synthetic solutions of Re were prepared by dissolving ammonium perrhenate (APR), Alamine304-1, Cyanex272 and $D_2EHPA$ were used solvent extractants distilled in kerosene. The extraction percentage of Re by Alamine304-1 was higher than the other extractants as Cyanex272 and $D_2EHPA$ and the percentage is about 99%. Only 99% of Re was extracted in the presence of Al, Co and V in HCl solution.

Acute Toxicities of Emulsifiable Concentrates and Granules of Valeriana fauriei Briquet and Alpinia galangal Swartz Essential Oils against Cyprinus carpio (길초근(Valeriana fauriei Briquet) 및 양강근(Alpinia galangal Swartz) 정유의 유제 및 입제제형의 잉어(Cyprinus carpio)에 대한 급성독성)

  • Jeon, Hwang-Ju;Kim, Kyeongnam;Kim, Yong-Chan;Lee, Sung-Eun
    • Korean Journal of Environmental Biology
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    • v.36 no.4
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    • pp.659-664
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    • 2018
  • In this study, two essential oils (EOs) extracted from Aleriana fauriei and Alpinia galangal were formulated as an emulsifiable concentrate (EC) and a granule. In the evaluation of their acute toxicity on fishes, Cyprinus carpio adults were used and the toxicities were determined in a static condition. The formulations were prepared from the essential oil extracted by three different methods namely steam distillation (SD), solvent extraction (SE) and supercritical fluid extraction (SFE). The acute toxicities were calculated using $LC_{50}$ values. Among EOs, only the EO extracted by solvent showed acute toxicities on carps. Some of the EC, EOs of Aleriana fauriei did not exhibit toxicity, while EOs from Alpinia galangal showed potent acute toxicities on carps. Among the granules, granules formulated with Aleriana fauriei EO extracted by SD method and Alpinia galangal EO extracted by SFE method showed acute toxicities on fishes. Nevertheless, $LC_{50}$ of ECs and granules formulated with all types of EOs in this study was higher than the fish toxicity level III for pesticides suggested by Korea Rural Development Administration. Furthermore, cytochrome P450 1A and glutathione S-transferase were confirmed as biomarkers in carps in response to the exposure to Alpinia galangal EO extracted by SD and SFE method, tracking Alpinia galangal EO in the aquatic environment.

Optimization of d-limonene Extraction from Tangerine Peel in Various Solvents by Using Soxhlet Extractor (다양한 용매에서 Soxhlet 추출기를 이용한 감귤 껍질에서 d-limonene 추출의 최적조건 연구)

  • Park, Sang Min;Ko, Kwan Young;Kim, In Ho
    • Korean Chemical Engineering Research
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    • v.53 no.6
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    • pp.717-722
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    • 2015
  • D-limonene included in citrus fruits is obtainable to extract essential oil as well as separate the oil ingredient. Soxhlet extraction, a type of SDE (Simultaneous steam Distillation and solvent Extraction), was used to extract limonene from tangerine peel. HPLC analysis was performed to quantify extracted d-limonene by using reversed-phase HPLC column. Results of HPLC analysis showed that the optimal extraction time was 2 hours in any solvent, and the extracted amounts of d-limonene in tangerine peel (per g tangerine peel) were 7.77 mg, 0.49 mg, and 0.28 mg in ethyl alcohol, n-hexane, and ether. Because yield was the highest in using ethyl alcohol as a solvent, polarity is stronger factor to effect on yield of extraction than boiling point.

A Study on Remediation Characteristics of Soils Contaminated with Co using Solvent Flushing Method (Solvent Flushing방법을 이용한 코발트로 오염된 토양의 제염특성에 관한 연구)

  • 김계남;원휘준;김희연;이병직;오원진
    • Journal of Korea Soil Environment Society
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    • v.4 no.2
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    • pp.55-62
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    • 1999
  • The solvent flushing apparatus for soil remediation was designed. After the soil around nuclear facilities was sampled and compulsorily contaminated by Co, the characteristics remediated by solvent flushing were analyzed. Meanwhile, the nonequilibrium sorption code was developed for modelling of the soil remediation by solvent flushing, input parameters needed for modelling were measured by laboratory experiment. Experimental results are as follows : The soil around nuclear facilities belongs to Silt Loam including a lot of silt and sand. When water was used as a solvent, the higher was the hydraulic conductivity. the higher the efficiency of soil remediation was. The values calculated by the nonequilibrium sorption code agreed with experimental values more exactly than the values calculated by the equilibrium sorption code. When EDTA solution was used as a solvent. the soil remediation efficiency by EDTA solution showed higher than that by water.

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