• Title, Summary, Keyword: 암컷비율

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LAMP(Loop-mediated Isothermal Amplification) 기법을 이용한 소 수정란 성판별

  • Jo, Sang-Rae;Choe, Seon-Ho;Kim, Hyeon-Jong;Choe, Chang-Yong;Jin, Hyeon-Ju;Jo, Chang-Yeon;Son, Dong-Su
    • Proceedings of the Korean Society of Embryo Transfer Conference
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    • pp.51-62
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    • 2007
  • 성판별을 위한 biopsy 후 수정란의 발달율 및 동결-융해 후의 생존율 조사는 다음과 같다. 한우 체내 및 체외 수정란의 성판별을 위해서 영양막 세포의 일부를 채취하기 위해서 수정란을 biopsy 하였다. biopsy된 수정란의 생존율 조사의 결과는 체내 수정란이 100% 그리고 체외수정란이 90.0%의 결과를 나타내어 체내수정란이 체외수정란보다 biopsy 후의 생존율이 높게 나타났음을 알 수 있었다. 수정란의 성판별 비율은 체내수정란에서는 암컷과 수컷의 비율이 46.3%와 53.7%로 각각 나타나 수컷의 비율이 암컷보다 다소 높은 경향을 보였으며, 체외수정란에 있어서는 암컷과 수컷의 비율은 40.0%와 60.0%로 수컷의 비율이 높게 나타났으나, 유의적인 차이는 보이지 않았다. 성 판별된 수정란의 동결-융해 후 생존성은 완만동결 방법에 의한 수정란의 생존율은 체내수정란에서 58.8%, 체외수정란에서는 41.7% 그리고 초자화 동결 방법에서는 체내수정란의 생존율이 77.8%, 체외수정란은 57.1%로의 결과를 보여 체내수정란을 이용한 초자화 동결 방법에서 상대적으로 더 높은 생존율을 보였다.

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Studies on the Comparisons between Korean Native Fowl and Broiler for the Live Performance and Carcass fields 1. Comparisons between Korean Native Fowl and Broiler for the Live Performance and Carcass Yields (한국재래닭과 육계의 발육 및 도체 특성 비교 연구 1. 재래닭과 육계의 발육 및 도체형질 비교)

  • 정익정;이병현;양창범;한성욱;정선부
    • Korean Journal of Poultry Science
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    • v.19 no.4
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    • pp.205-215
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    • 1992
  • This study investigated the characteristics of Korean Native Fowl (KW) to standarize the feeding regime and compared with those of broiler. The characteristics of KNF studied were meat productivities and carcass traits. The data were collected for 14 weeks from March to June, 1992, by recording from 200 birds(50 birds per each sex and 2 breed) were as follows : 1. Average body weight at 14 weeks of age of Korean Native Fowl(KW) and broiler were 868.7g and 3,526.6g, respectively and were 26.6 times and 72.9 times of day old chicks weight averages, respectively. 2. The carcass yields during 9∼14 wks were 74.1∼78.5% for KNF male, 73.g∼77.5% for KW female, 72.2∼76.9% for broiler male and 71.7∼76.4% for female. It was shown that carcass yields of KNF was 2% higher than that of broiler. 3. In edible portion, the yields of wings weight to carcass weight were 11.5∼12.4% for KNF male, 11.5∼13.1% for KNF female, 10.7∼11.9% for broiler male and 10.7∼12.2% for broiler female, it was shown that wings weight of KW was higher than that of broiler and wings weight of KNF during 9∼14 wks. 4. The portions of back and breast weight were higher than those of KNF during 9∼14 wks. The back portions in carcass weight were 15.4∼17.0% for KW male 15.4∼16.8% for KNF female, 17.2∼19.0% for broiler male, and 17.2∼19.0% for broiler female. The breast portions in carcass weight were 19.6∼22.0% for KNF male, 20.2∼22.7% for KNF female 23.S∼24.5% for broiler male, and 25.1∼27.7% for broiler female. 5. The portion of thigh and drumsticks weight in carcass weight of KNF was similar to that of broiler. They were 26.8∼28.0% for KNF male, 25.5∼27.1% for KNF female, 27.7% for broiler male, and 26.9% for broiler female, respectively. 6. For the weight of inedible portion during 9∼14 wks, feather weight of KNF was 5.3∼6.8% of live weight and 3.8 ∼4.4% in those of broiler. This result indicated that KNF has more rooms for improvement than broiler. 7. Abdominal fat weight was 2.42∼3.88g for KNF male and 1.84∼3.08g for KNF female, which was 0.5% of carcass weight. But broiler has more abdominal fat than KNF as 39.4∼56.3 g for male, 33.3∼67.8 g for female which was 2.5 ∼3.0% of carcass weight.

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Reproductivity of Mixtures of Race 3 and Race 4 of Heterodera glycines on Soybean Cultivars (대두품종에서의 콩씨스트 선충 혼합 Race의 생식력)

  • Kim Young Ho;Riggs Robert D.
    • Korean Journal Plant Pathology
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    • v.3 no.4
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    • pp.245-251
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    • 1987
  • Soybean cultivars were inoculated with mixtures of races 3 and 4 of the soybean cyst nematode(SCN), Heterodera glycines Ichinohe. On a susceptible soybean cultivar Lee, the relative female maturation of the race mixture was not significantly different when compared with the maturation potentials for each of the two races alone. However, female maturation was significantly lower for the mixture than' for race 4 alone on the soybean cultivas Pickett and Mack which are susceptible to only race 4. Selection of SCN populations consisting of race 3 and race 4 resulted in a decrease in race 4 on soybean cultivars Lee and Bragg which are susceptible to all SCN races and an increase in race 4 on soybean cultivars Pickett and Peking which are susceptible to only race 4. A significant reduction of race 4 was also observed on Lee with most mixtures of race' 3 and race' 4 combined in several ratios, suggesting that race 3 was more competitive than race 4 on the susceptible soybean.

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Studies on the Production of All-Female Populations of Olive Flounder, Paralichthys olivaceus IV. Sex Determination by Water Temperature During Sex Differentation Period (넙치 전 암컷 집단의 생산을 위한 연구 IV. 성분화 기간 중 수온에 의한 성 결정)

  • Kim Kyung-Kil;Bang In Chul;Kim Yoon
    • Journal of Aquaculture
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    • v.9 no.4
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    • pp.429-435
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    • 1996
  • We investigated the effects of rearing water temperatures during sex differentiation period on sex ratios in olive flounder, Paralichthys olivaceus. Control and gynogenetic diploid juvenile flounder reared at water temperature of 18, 21, 24 and $27^{\circ}C$ for 65 days from 35 to 100 days after hatching. Fish were sampled to examine sex ratios at 195 or 260 days after hatching. Female ratios of control and gynogenetic diploid flounder declined rapidly as water temperature increased. Sex ratio of gynogenetic diploid reared at $27^{\circ}C$ was very close to 1 : 1 ratio (P<0.01), The survival rates of control and gynogenetic diploid reared at $27^{\circ}C$ were different from other water temperature groups. The growth of body weight of control and gynogenetic diploid reared at $18^{\circ}C$ were different from other water temperature groups.

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The Effect of Temperatures on the Biological Characteristics of Two Aphid Parasitoids Aphelinus asychis (Walker) and Aphelinus varipes (Förster) (Hymenoptera: Aphelinidae) on Two Aphid Hosts (진딧물 기생봉 목화면충좀벌과 진디면충좀벌의 기주와 온도에 따른 생물학적 특성 비교)

  • Kang, Eun Jin;Byeon, Young-Woong;Kim, Jeong-Hwan;Choi, Man-Young;Choi, Yong-Seok
    • Korean journal of applied entomology
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    • v.51 no.4
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    • pp.397-403
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    • 2012
  • This study compared the Aphelinus varipes and Aphelinus asychis in terms of how they parasitized the cotton aphid, Aphis gossypii and green peach aphid, Myzus persicae. Host-feeding, parasitism, emergence, the proportion of females and development time were all studied at 15, 20, 25 and $30^{\circ}C$ in controlled climate cabinets. When A. gossypii were provided for the two aphid parasitoids, the number of aphids killed by host-feeding for A. varipes (5.4 and 9.7 aphids) at $15^{\circ}C$ and $25^{\circ}C$ was higher than those for A. asychis (2.0 and 2.9 aphids). At $15^{\circ}C$ and $30^{\circ}C$, the parasitized A. gossypii were higher in A. varipes (11.1 and 21 aphids) than in A. asychis (7 and 12.3 aphids). The emergence rate was also significantly different between A. varipes (83.3%) and A. asychis (69.4%). The proportion of females was higher for A. asychis (75.2 and 73.9%) than for A. varipes (19.5 and 48.6%) at $15^{\circ}C$ and $30^{\circ}C$, respectively. No significant differences were found in development time between the two parasitoids. When M. persicae were provided for the two parasitoid species, the host-feeding number and the emergence rate of two parasitoids were not different at all four temperatures. The M. persicae were more highly parasitized by A. varipes (12.1 and 17.1 aphids) than by A. asychis (6.1, 10 aphids) at 20 and $25^{\circ}C$. The proportion of females for A. varipes (65.3 and 90.0%) was higher than that for A. asychis (34.4 and 78.8%) at $15^{\circ}C$ and $25^{\circ}C$. The development time from oviposition to the adult emergence of A. varipes (19.9 d) was significantly longer than that of A. asychis (16.5 d) at $20^{\circ}C$. Development times decreased with increasing temperature for both in two parasitoid species.

Morphometric Characteristics and Gonad Maturity of Snow Crab, Chionorcetes opilio in the Eastern Coast of Korea (동해안 대게, Chionoecetes opilio의 크기조성 특징과 성숙)

  • 임영수;이종하;이종관;이복규;허성범
    • Journal of Aquaculture
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    • v.13 no.3
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    • pp.245-251
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    • 2000
  • Carapace width (CW) of main size class of the snow crab was 70~90 mm for females and 80~100 mm for males. The CW holds a stronger relation with body weight than that of carapace length (CL). The number of eggs attached to the pleopods was in the range of 35,000 and 114,000 eggs in crabs with 65 and 88 mm CW, respectively). The logistic maturity curve on morphometric measurements and gonad examination, defined by the presence of egg brooding in wide abdomen of the female and spermatophores inside the vasa deferentia of the male indicated that 50 % of crabs attained gonadal maturity at 54.2 mm and 58.8 mm CW for females and males, respectively. In the relationship between CW and body weight, a sharp break at 53~55 mm CW indicates that morphological changes associated with maturity have occurred.

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Sex Ratio of Olive Flounder, Paralichthys olivaceus Populations Collected from Stocked Areas in Southwestern Sea of Korea (우리나라 남서해 종묘방류해역에서 어획된 넙치집단의 성비)

  • Jeong, Dal-Sang;Min, Byung-Hwa;Ha, Dong-Soo;Kim, Chul-Won;Kang, Chang-Soo;Myeong, Jeong-In
    • Journal of Fisheries and Marine Sciences Education
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    • v.27 no.2
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    • pp.363-370
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    • 2015
  • Olive flounder, Paralichthys olivaceus is one of the most target fish for aquaculture and national stock enhancement program in Korea. In order to find more information about hatchery fish stocking for stock enhancement we investigated the sex ratio of wild-caught fish populations which were artificially released into wild seas. The recapture rate was 9.0% to 94.5% in studied areas. The proportion of sex ratio ranged from 7.8% to 64.6% in female and from 34.4% to 92.1% in male respectively. This result suggests that the sex ratio of wild populations might be effected by the released fish. So we need more careful stocking program of the fish to preserve the balance of the sex ratio in the wild population.

Reproductive Ecology of the Freshwater Melania Snail, Semisulcospira coreana (v. Martens) in Bukhan River (북한강 참다슬기, Semisulcospira coreana (v. Martens) 의 번식생태)

  • Kim, Dae-Hee;Bang, In-Chul;Lee, Wan-Ok;Baek, Jae-Min
    • The Korean Journal of Malacology
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    • v.28 no.2
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    • pp.175-185
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    • 2012
  • To clarify reproductive ecology of the melania snail Semisulcospira coreana (v. Martens, 1886) in Bukhan River, gonad development, fatness, gonad index, sex ratio, first sexual maturity of population, monthly change of larvae number and developmental stages in brood pouches were investigated by six identification methods. As maturation progrsses, the sex of the snali can be distinguishable easily by color:: the ovary being blue-green and testis light yellow. The sex ratio of female to male individuals over 13.95 mm shell height was significantly different from 1:1 (${\chi}^2$ = 38.45, p < 0.05). The sex ratio of female to male individuals changed drastically according to the season, Based on the monthly variations of fatness, gonad index and histological analysis, spawning occurred twice a year (spring and autumn) and the mean size of matured eggs was $450{\mu}m$ in diameter. The monthly change of larval number in brood pouch showed also two distinct peaks in March and September during the year. The average number of larvae in brood pouches was 286 - 862 individuals. In this study, the number of larvae in the brood pouches were a minimum in December and a maximum in March (975 larvae). The biological minimum size (the size at 50% of group sexual maturity) of the melania snail was 13.95 mm in shell height in females and males. All females over 15 mm in shell height possesed brood pouches.

Biological Properties of Common Squid in the Yellow Sea of Korea (황해에서 어획된 살오징어의 생물학적 특성)

  • Choi, Kwang-Ho;Kwon, Dae-Hyeon
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.17 no.4
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    • pp.367-373
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    • 2011
  • We investigated the biological characteristics of the squid, Todarodes pacificus Steenstrup through the samples caught monthly in the Yellow Sea($32^{\circ}$ 30'N, $122^{\circ}E-37^{\circ}$ 30'N, $127^{\circ}E$) from 1985 to 1987. Monthly mantle length frequency were separated into Autumn spawning group and winter spawning group with Tanaka's method(1956) to determine the main group of the squids caught in the Yellow Sea. The ratio of winter spawning group was higher than that of autumn spawning group. Monthly gonadosomatic index of females increased on July in 1985, on October in 1986, and October in 1987 and decreased later in each year. That of males increased on June in 1985, on July in 1986, and June in 1987. The length of 50% group maturity of female was 20.7 cm mantle length(ML) in 1985, 17.9 cm ML in 1986, and 17.2 cm ML in 1987. The ratio of female was higher than that of male in the group over 24 cm ML, and the ratio of male was higher than that of female in the group under 24 cm ML. We concluded that the length of 50% group maturity of squids was affected by the density of the population status.

Survival Rate, Developmental Competence and Sex Ratio of Post-thawed Hanwoo Embryo Following Biopsy (한우 수정란의 Biopsy 후 배발달율과 동결-융해후 생존성 및 성비)

  • Cho, S.R.;Choi, S.H.;Kim, H.J.;Choe, C.Y.;Jin, H.J.;Cho, C.Y.;Son, D.S.
    • Journal of Animal Science and Technology
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    • v.49 no.2
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    • pp.287-294
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    • 2007
  • The present study was to conducted the sexing efficiency and accuracy of bovine embryo by LAMP (Loop-mediated isothermal amplification) method, the development of the biopsied embryos into re- reformation and the freezability of these blastocysts by slow-freezing and vitrification. In vivo embryos were superovaluted with gonadotropin(Antorin R-10) for 4 days combined with progestrone releasing intravaginal(CIDR) insertion in Hanwoo donors, and in vitro embryos were used blastocyst embryos at Day 7 or Day 8 after post-insemination in vitro. The biopsy of bovine embryo was carried out in a 80μl drop with Ca2+-Mg2+ free D-PBS and the viability of biopsied embryos were evaluated in IVMD medium at over 12 h culture time in 5% CO2 incubator.For embryo sexing, about five or seven blastomeres were isolated from in vitro and in vivo embryos at blastocysts with microblade. and were then subjected to LAMP. The survivability of biopsied embryos were no difference in the development rate to re-formation of blastocysts between in vivo and in vitro embryos(100% and 90% respectively). The rates of sexed embryos were compared according to two groups, the female rate was lower than that the male in the in vivo and in vitro embryos(46% vs, 54% and 40% vs, 60%, respectively). However, there were no difference in the overall sexing ratio between the two groups. The survivability of frozen-thawed sexed embryos were lower in the in vitro than in vivo embryos in the slow-freezing(Group 1) and vitrification method(Group 2), (41.7% vs. 58.8% and 57.1% vs, 77.8. respectively).