• Title, Summary, Keyword: 실시간

Search Result 25,823, Processing Time 0.1 seconds

Effect of Various Forms of Floor System on Performance of Meat-type Duck and Environments of Duck House (오리사 바닥 형태가 육용오리의 생산성 및 사육환경에 미치는 영향)

  • Bang, Han-Tae;Kim, Dong-Woon;Hwangbo, Jong;Na, Jae-Cheon;Kang, Hwan-Ku;Kim, Min-Ji;Mushtaq, M.M.H.;Parvin, R.;Choi, Hee-Chul;Lee, Sang-Bae;Kang, Min;Kim, Ji-Hyuk
    • Korean Journal of Poultry Science
    • /
    • v.40 no.3
    • /
    • pp.253-262
    • /
    • 2013
  • This experiment was conducted to investigate the effects of floor type and heating system on performance, housing environment and health status of ducks reared in three types of duck house (OD : Open floor house-Direct heating system, OF : Open floor house-Floor heating system and LD : Loft type house-Direct heating system). In OF treatment, PVC pipes were installed for heating under concrete floor and covered with litter. In LD treatment, plastic mesh was installed 50 cm above the floor so that duck's droppings can pass through it. Each treatment had four replicates of 25 birds (Cherry Valley duck breed) per pen. There were no significant differences in weight gain and feed intake of ducks for 6 weeks among all treatments. However, feed conversion ratio in LD was significantly higher (p<0.05) than that in OF. No differences were found in carcass charac- teristics, with the exception of abdominal fat weight where OF were higher than the others. Concentrations of $CO_2$ and $NH_3$ gas in OD were higher than those of OF and LD at 3, 4 and 5 weeks. Moisture content in litter of OF was lower than that of OD. In contrast, the amount of dust in the air was higher in OF than in OD. The amount of fuel used for 6 weeks in LD was lower about 21% than that in OD. Some of unusual symptoms were observed in open floor house and loft type house, such as lying, spraddle legged, twisted ankle and legs, wounded sole, or etc. No components of leukocyte and erythrocyte of blood were significantly different among all treatments. The results of this experiment showed that OF and LD systems had no positive effects on performance of meat type commercial duck. However, there were some positive effects of certain house type for the improvement of environmental condition in duck house for hygienic production. In the future, more research on the effect of various facilities and systems for duck house is needed.

Establishment of Reference Range of Proinsulin (Proinsulin 참고치 설정에 관한 연구)

  • Nam, Yee Moon;Shin, Yong Hwan;Kim, Ji Young;Seok, Jae Dong
    • The Korean Journal of Nuclear Medicine Technology
    • /
    • v.17 no.1
    • /
    • pp.76-79
    • /
    • 2013
  • Purpose: It is very important to establish the appropriate reference range in the laboratory for preventing mistakes like false positive or false negative. Because the reference range in the laboratory is standard of patient test results interpretation. Proinsulin is precursor hormone of insulin, and the importance is increasing for diagnosing diabetes or insulinoma. Proinsulin reagent used in our laboratory is produced in the USA, and the reference range provided by manufacturer was adapted to our reference range after the validation test. But, it is generally recommend for the every laboratory to establish the their own reference range. So, we decided to re-evaluate the reference range with our patients' test results. Materials and Methods: Among 737 patients who had been to health promotion center in our hospital between Dec. $8^{th}$ 2011 and Dec. $21^{st}$ 2011, 563 patients are chosen with exception of diabetics patients and patients showing abnormal test results in Fasting Glucose, HbA1c, Insulin, and C-peptide. The 563 test results (275 males and 288 females) were classified with three groups(entire, male, female), and analysis of normal distribution was performed with aid of SPSS(version 19.0). Because Each group didn't show normal distribution, the reference range was set from the lowest limit of 2.5% to the highest limit of 97.5% with Percentile method used in non-normal distribution. Results: When evaluation values are sorted in ascending order, the entire range is 4.5~52.0 pM and 5.3~51.9 pM for male and 4.5~52.0 pM for female. The calculated reference range with percentile method shows 6.7~26.5 pM for entire group, 6.8~26.5 pM for male and 6.7~26.5 pM for female, respectively. Conclusion: The reference range provided by reagent manufacturer is 6.4~9.4 pM and the one established in this study is 6.7~26.5 pM. This difference might be caused by racial characteristics between Western people and Koreans. So an ideal reference range can be gotten with normal population visiting to every hospital. Our hospital has been using the newly re-establishing reference range under consultation with the department of endocrinology since Aug. $1^{st}$ 2012.

  • PDF

Analysis of the Genetic Diversity of Radish Germplasm through SSR Markers Derived from Chinese Cabbage (배추 SSR 마커를 이용한 무의 육성 계통 및 수집종의 유전적 다양성 분석)

  • Park, Suhyoung;Choi, Su Ryun;Lee, Jung-Soo;Nguyen, Van Dan;Kim, Sunggil;Lim, Yong Pyo
    • Horticultural Science & Technology
    • /
    • v.31 no.4
    • /
    • pp.457-466
    • /
    • 2013
  • Since the early 1980s, the National Institute of Horticultural & Herbal Sciences has been breeding and collecting diverse radish breeds to select those samples with better horticultural characteristics, to ultimately expand and develop as good radish produce. Genetic diversity is a crucial factor in crop improvement and therefore it is very important to obtain various variations through sample collection. The collected samples were compared with one another in order to assess the level of diversity among the collections, and this procedure allowed for increased application of the gathered resources and aided in determining the direction to secure further samples. Towards this end, this experiment was conducted in order to examine whether the SSR markers derived from Chinese cabbage samples could be transferred to the radish samples. Among the radish breeding lines and introduced resources, 44 lines were used as materials to analyze the genotype using 22 SSR markers selected. As a result, the analysis showed that among all the selected markers, 'cnu_m139' and 'cnu_m289' were the most useful markers for diversity evaluation. The genetic relationship of the radish genetic resources showed that the geographic origins affected the diversity. Furthermore, the different types of radish groups were also determined by the year they were bred. This result demonstrated that there are differences between the older radish breeds and the more recently developed radish breeds. Even though a relatively small number of markers were used in the analysis, it was possible to distinguish whether the radish was bred 30 years ago or in the 2000s, and that the similar physical shapes comprised a particular group, showed that the SSR markers can indeed be successfully applied to to study the diversity within radish breeding lines. Through the results of this study, it can be concluded that the SSR marker developed for the Chinese cabbage can be applied to examine the genetic diversity and analyze the relationship (genetic resource determination) of radish.

Comparison of Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, and Loop-Mediated Isothermal Amplification for the Detection of Cronobacter sakazakii in Milk Powder (분유에 오염된 Cronobacter sakazakii 검출을 위한 중합효소연쇄반응, 실시간중합효소연쇄반응, 등온검출법의 비교)

  • Kim, Young-Joo;Seo, Sheungwoo;Wang, Xiaoyu;Seo, Dong Joo;Lee, Min Hwa;Son, Na Ry;Lee, Bog-Hieu;Choi, Changsun
    • Food Science of Animal Resources
    • /
    • v.33 no.5
    • /
    • pp.610-616
    • /
    • 2013
  • Loop-mediated isothermal amplification (LAMP) is an emerging detection technology for the amplification of DNA under isothermal conditions. The aim of this study was to develop a rapid and reliable LAMP technique for the detection of Cronobacter sakazakii in milk powder. In order to enhance the sensitivity and specificity, LAMP primers targeting outer membrane protein A (ompA) gene of C. sakazakii were designed using Explorer V4 software. Thirty seven C. sakazakii strains and 13 pathogenic microorganisms were used for comparative detection of C. sakazakii using polymerase chain reaction (PCR), real-time PCR, and LAMP. LAMP developed in this study could specifically detect C. sakazakii strains without cross-reactivity with other foodborne pathogens. LAMP products amplified from ompA gene of C. sakazakii were digested with with HhaI and NruI enzyme. The specificity of LAMP was confirmed by restriction fragment length polymorphism (RFLP) analysis. LAMP could detect C. sakazakii within 1 h without bacterial culture and its detection limit was as low as 1 CFU/mL C. sakazakii in milk. In the comparison of the sensitivity, LAMP showed 10,000- and 100-times higher detection limit than PCR or real-time PCR, respectively. Therefore, this study can conclude that LAMP is a rapid and reliable detection technique for C. sakazakii contaminated in powdered milk.

Comparison of Consumption of Processed Food and Personality of Middle School Students on Nutrition Education (영양교육이 중학생들의 가공식품 섭취 및 인성특성에 미치는 영향 비교)

  • Lee, Kyung-Ock;Cho, Eun Ju
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.42 no.10
    • /
    • pp.1600-1607
    • /
    • 2013
  • The aim of this study was to investigate effect of nutrition education methodology on consumption of processed food and personality of middle school students aged 14 to 16 years who live in Busan. We categorized questionnaire based on students' consumption of processed food and personality characteristics. Based on the above, we determined serum mineral level to validate the correlation between personal characteristics and quantifiable serum mineral level. Following adoption of a proper nutrient education methodology, we found that consumption of processed foods such as meat and meat products, frozen food, noodles, bread and snacks, and drink was significantly reduced. In addition, there were distinct changes in personality characteristics such as stability, governmentality, sociality, and responsibility that became markedly increased, whereas impulsiveness decreased. We found that these distinct nutrition- education-based changes were statistically significant, when compared before and after providing proper nutrition education. We interpret these results as group with low stability showed a high consumption of meat and meat products, noodles, bread and snacks, and drink, whereas group with high stability showed a high consumption of milk and dairy products. Similarly, we also found that group with low impulsiveness showed a high consumption of canned foods, whereas group with high impulsiveness showed a high consumption of meat and meat products, frozen food, noodles, bread and snacks. Our results indicated that serum Na after nutrition education decreased, whereas other minerals including Ca, Fe, Zn, Mg and K increased, when compared with before nutrition education procedure. This study suggests that nutrition education methodology and establishment of proper dietary educational programs keeping in view balanced dietary habits for middle school students would not only appear to make them get along with healthy dietary habits, but also change their personality characteristics.

Glomerular Filtration Rate Measurements Using $^{99m}$Technetium-mercaptoacetyltriglycine Dynamic Renal Scintigraphy in Children with Renal Disease (신장 질환이 있는 소아에서 $^{99m}$Technetium-mercaptoacetyltriglycine ($^{99m}Tc$-MAG3) 신장 스캔에 의한 사구체 여과율 측정)

  • Yoon, In Ae;Yun, Ki Wook;Lim, In Seok;Choi, Eung Sang;Yoo, Byung Hun
    • Childhood Kidney Diseases
    • /
    • v.17 no.2
    • /
    • pp.57-64
    • /
    • 2013
  • Purpose: In children, 24-hour urine collections are unreliable for evaluating glomerular filtration rate (GFR) because of the difficulty of regulating voiding and the daily variation of urinary creatinine up to 25%. Additionally, creatinine clearance (Ccr) based on urinary creatinine is considered inaccurate. The purpose of this study was to compare estimated GFR determined using Ccr, formulas with serum cystatin C and creatinine, and $^{99m}Tc$-mercaptoacetyltriglycine (MAG3) dynamic renal scintigraphy. Methods: This retrospective study included 101 patients (age, <18 years) who visited Chung-Ang University Hospital between July 2011 and August 2012. GFR was estimated using 24-hour urinary creatinine, five formulas with serum creatinine and cystatin C, and $^{99m}Tc$-MAG3 renal scan. Results: Of the 101 patients, glomerular renal diseases were present in 60 patients (59.4%) and non-glomerular diseases were present in 41 patients (40.6%). There was a significant correlation between estimated GFR determined using $^{99m}Tc$-MAG3 renal scan and Ccr (r=0.389, P <0.001). The correlation values between estimated GFR determined using $^{99m}Tc$-MAG3 renal scan and each formula of Schwartz, Counahan-Barratt, Cockcroft-Gault, Filler and Lepage, and Bokencamp were 0.265 (P=0.007), 0.128 (P=0.044), 0.230 (P=0.021), 0.356 (P<0.001), and 0.355 (P <0.001), respectively. $^{99m}Tc$-MAG3 renal scan was correlated with estimated-GFR by all formulas in decreased renal function. Conclusion: Estimated GFRs determined using serum creatinine and cystatin C, and $^{99m}Tc$-MAG3 renal scan correlated well with estimated GFR determined using Ccr. $^{99m}Tc$-MAG3 renal scan may be replaced for evaluation of renal function with convenience in patients with renal disease and decreased renal function in childhood.

A Study on the Attraction Factors of Eco-city using Importance-Satisfaction Analysis - The Case of Suncheon City - (중요도-만족도(ISA) 분석을 활용한 생태도시 매력요인에 관한 연구 - 전남 순천시를 대상으로 -)

  • Lee, Jeong;Kim, Sa-Rang
    • Journal of the Korean Institute of Landscape Architecture
    • /
    • v.42 no.2
    • /
    • pp.52-64
    • /
    • 2014
  • I the recent years, Seoul, Daejeon, Changwon, and Suncheon have started to strengthen P.R. efforts on eco-brands produced by the city and to publicize as a specialized tourist city in an attempt to change their identity and image. However, there is actually a question whether the efforts of the local governments have any direct impact on satisfaction with urban living environments and the attractions of the city. The purpose of this study was to examine the awareness of residents and visitors about the attractions of Suncheon City as an eco-city and to discuss the planning criteria for the eco-city brand building and its management. The research data was collected in Suncheon City and main results of this study are as follows. The residents and the visitors investigated were satisfied with the environmental friendliness of this city and regarded it as an eco-city. As a result of asking them why they viewed the city as an eco-city, many of the residents cited diverse green tracts of land as the reason, whereas the visitors replied they were satisfied with the state of marshy areas preserved by the city. The psychological factors related to the satisfaction of the eco-city by the residents were composed of four factors, 'cultural factor', 'urban infrastructure factor', 'ecological factor' and 'scenery factor'. The visitors were composed of five factors, 'cultural factor', 'urban infrastructure factor', 'ecological factor', 'scenery factor' and 'amenity factor'. Out of the factors, the cultural factor and the urban infrastructure factor were found to exert the largest influence on the overall satisfaction of the residents and the visitors. The ISA(Importance-Satisfaction Analysis) was made, the residents and the visitors gave top priority to 'diversity of natural attractions', 'pleasant season and weather', 'beautiful scenery', 'diversity of rare animals and plants', 'diversity of parks', 'green areas and streets', 'broad ecological area' and 'the preservation of marshy areas' among the attractions of the eco-city. They placed importance on the activation of green traffic and walking environments as well, but they weren't satisfied with the state of the two in the city. Therefore there was much room for improvement in that regard.

The Influence of Accommodation on Watching Home 3D TV at Close Distance (가정용 3D TV의 근거리 시청이 조절기능에 미치는 영향)

  • Kim, Jung-Ho;Hwang, Hae-Young;Kang, Ji-Hun;Yu, Dong-Sik;Kim, Jae-Do;Son, Jeong-Sik
    • Journal of Korean Ophthalmic Optics Society
    • /
    • v.18 no.2
    • /
    • pp.157-163
    • /
    • 2013
  • Purpose: This study was investigated weather watching 2D and 3D images effecting on accommodative function (AF), and differences between changes of AF by 2D and 3D. Methods: 50 subjects (male 30, female 20) aged 20's to 40's years old ($22.9{\pm}3.93$ years) who are available to watching 3D images were participated for this study. Accommodative amplitude (AA) by near point of accommodation (NPA), accommodative response (AR), positive and negative relative accommodation (PRA, NRA), accommodative facility (AF) were measured before, after watching 2D and 3D images at 1 m distance for 30 minutes respectively. Results: Accommodative amplitude after both watching 2D and 3D images decreased comparing to before watching images, and AA after watching 3D images was significantly lower than after watching 2D images. AR after both watching 2D and 3D images increased comparing to before watching images, but there was no difference between 2D and 3D. PRA and NRA were not significantly different between before, after watching 2D and 3D images. Accommodation speed by AF was increased for before watching ($13.52{\pm}3.32$ cpm) following by for after watching 2D images ($14.28{\pm}3.21$ cpm) and for watching 3D images ($14.90{\pm}3.27$ cpm). Conclusions: Watching images at close distance is effect to accommodation functions, and sequence of AA decrease of before watching images following by after watching 2D images and after watching 3D images may effect to asthenopia with same sequence as AA decrease. The results of increase of AF after watching images, specially 3D images show a possibility of vision therapy and further detail VT studies using 3D images are required in the future.

Survey on Comparative Awareness between Media and Industry on Occurrence of Food Safety Incident (식품안전사건 발생 시 미디어와 산업의 인식도 조사)

  • Shin, Won-Jung;Lee, Yu-Si;Oh, Se-Ra;Park, Tae-Gyun;Kim, Renee;Kim, Ho-Sik;Lee, Jung-Ho;Lee, Seung-Yong;Ha, Sang-Do
    • Journal of Food Hygiene and Safety
    • /
    • v.28 no.2
    • /
    • pp.108-114
    • /
    • 2013
  • To describe the comparative awareness between media and industry, we conducted a survey with a professional group including 28 food safety managers at food corporations and 17 food journalists to find out the differences in perception of 'reporting terms' and 'correspondence terms' when food safety incident occurs. We surveyed by questionnaire methods and did the statistical analysis by SPSS. The results showed that 70.6% of the journalists and 92.9% of the corporation workers answered 'yes indeed' about the question whether the media has a great influence when food safety incident occurs. In the perception that use of the stimulating terms of the media was the cause of company damage, 83% of the corporate workers answered 'yes indeed', and 70.6% of the journalists answered 'yes'. It means that both groups agreed on stimulating terms causing company damage. In the question whether the 'reporting terms' of the media and the 'correspondence terms' of the government and academia made a difference in the consumers' perception and the spread of the incident, 92.9% of the corporate workers and 35.3% of the journalists answered 'yes indeed'. It means that both groups showed a common opinion that the difference in choice of words expanded the perception of consumers, and the spread of the incident. However, the corporate workers agreed with this in a more serious manner than the journalist group. In standardization of 'reporting terms' and restricting use, corporate workers were overall at a positive stand while journalists showed a big difference in opinion within the group. In conclusion, it is necessary to establish a measure to standardize the terms used by corporations, journalists, and professionals in order to decrease the differences of terms and the damage of consumers and the company when food safety incident occurs.

Development and Validation of an Analytical Method for Ametoctradin Residue Determination in Domestic Agricultural Commodities by HPLC-PDA (HPLC-PDA를 이용한 국내 유통 농산물 중 ametoctradin 잔류량 분석법 개발 및 검증)

  • Do, Jung-Ah;Kwon, Ji-Eun;Lee, Eun-Mi;Kim, Mi-Ra;Kuk, Ju-Hee;Cho, Yoon-Jae;Kang, Il-Hyun;Kim, Hyung-Su;Kwon, Kisung;Oh, Jae-Ho
    • Korean Journal of Food Science and Technology
    • /
    • v.45 no.3
    • /
    • pp.285-292
    • /
    • 2013
  • This study was carried out to validate the safety of ametoctradin residues in agricultural commodities by developing an official analysis method. An analytical method was developed and validated using HPLC-PDA detectors. The samples were extracted with methanol, subsequently partitioned with dichloromethane and purified with florisil column chromatograph using acetone/hexane (30/70, v/v) as solvent. The method was validated by using grape, hulled rice, mandarin, and potato spiked with ametoctradin at 0.05 and 5.0 mg/kg, and pepper at 0.05 and 2.0 mg/kg. Average recoveries were 76-114.8% with relative standard deviation less than 10%, and the limit of detection and limit of quantification were 0.0125 and 0.05 mg/kg, respectively. The result of recoveries and overall coefficient of variation of the laboratory results from Gwangju regional Food and Drug Administration (FDA) and Daejeon regional FDA was accorded with Codex Alimentarius Commission Guideline (CAC/GL 40). Based on these results, this method was found to be appropriate for ametoctradin residue determination and can be used as the official method of analysis.