• Title, Summary, Keyword: 벼멸구

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친환경농업 - 친환경제재를 이용한 벼멸구 방제효과

  • Baek, Chae-Hun;An, Jeong-Gu
    • 농업기술회보
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    • v.49 no.5
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    • pp.16-16
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    • 2012
  • 벼멸구를 방제하기 위해 친환경농자재를 이용할 때 중요한 것은 방제시기와 방제횟수이다. 대부분의 친환경농자재는 벼멸구의 알을 죽이지 못하므로 벼멸구 발생초기에 10일 간격으로 2회 이상 처리해야 살아남은 알에서 새로 부화한 벼멸구까지 방제가 가능하다. 농가에서 주로 사용하는 친환경농자재의 벼멸구 방제효과에 대해 알아보자.

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Discrimination Method of Brown Planthopper, Nilaparvata lugens(Stal) Nymphs by the Fluorescent Spots between Compound Eyes in Rice Paddies (벼논에서 벼멸구(Nilaparvata lugens Stal)약충 두부의 형광성 반점에 의한 식별법)

  • 조성래;이동운;추호렬;박정규;신현열;김형환
    • Korean journal of applied entomology
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    • v.42 no.4
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    • pp.361-365
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    • 2003
  • Several species of planthoppers such as brown planthopper, N. lugens (Stal) (BPH), smaller brown planthopper, Laodelphax striatellus (Fall n) (SBPH), and white-backed planthopper, Sogatella furcifera (Horv th) (WBPH) are distributed in Korean rice paddies but not easy to discriminate them in situ. Accurate discrimination of them is an indispensable process in the forecasting for their outbreak and control. Especially, innovative discrimination method for BPH was required because BPH was one of the most important insect pest of rice. Nymphs and adults of BPHs, SBPHS, and WBPHS, thus, were examined their morphological characteristics in the paddies and laboratory. The nymphs of BPH had different characters from those of SBPH and WBPH. The nymphs of BPH had white fluorescent spot between bottom of compound eye and antenna, while there was no that spot the other two species. The white spot was the brightest at the nymphs just after hatching and getting weaker as the nymph developed. At last the white spot was totally disappeared at the adult stage. This white spot was innovative criterium to discriminate nymphs of BPH, SBPH, and WBPH in rice paddies.

ITS2 DNA Sequence Analysis for Eight Species of Delphacid Planthoppers and a Loop-mediated Isothermal Amplification Method for the Brown Planthopper-specific Detection (멸구과 8종의 ITS2 DNA 염기서열 비교 분석과 고리매개등온증폭법(LAMP)을 이용한 벼멸구 특이 진단법)

  • Seo, Bo Yoon;Park, Chang Gyu;Koh, Young-Ho;Jung, Jin Kyo;Cho, Jumrae;Kang, Chanyeong
    • Korean journal of applied entomology
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    • v.56 no.4
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    • pp.377-385
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    • 2017
  • Estimates of evolutionary sequence divergence and inference of a phylogenetic tree for eight delphacid planthopper species were based on the full-length nucleotide sequence of the internal transcribed spacer 2 (ITS2) region. Size of the ITS2 DNA sequence varied from 550 bp in Sogatella furcifera to 699 bp in Nilaparvata muiri. Nucleotide sequence distance ($d{\pm}S.E.$) was lowest between N. muiri and N. bakeri ($0.001{\pm}0.001$), and highest between Ecdelphax cervina and Stenocranus matsumurai ($0.579{\pm}0.021$). Sequence distance between N. lugens and other planthoppers ranged from $0.056{\pm}0.008$ (N. muiri) to $0.548{\pm}0.021$ (S. matsumurai). In the neighbor-joining phylogenetic tree, all planthoppers were clustered separately into a species group, except N. muiri and N. bakeri. The ITS2 nucleotide sequence of N. lugens was used to design four loop-mediated isothermal amplification (LAMP) primer sets (BPH-38, BPH-38-1, BPH-207, and BPH-92) for N. lugens species-specific detection. After the LAMP reaction of three rice planthoppers, N. lugens, S. furcifera, and Laodelphax striatellus, with the four LAMP primer sets for 60 min at $65^{\circ}C$, LAMP products were observed in the genomic DNA of N. lugens only. In the BPH-92 LAMP primer set, the fluorescence relative to that of the negative control differed according to the amount of DNA (0.1 ng, 10 ng, and 100 ng) and incubation duration (20 min, 30 min, 40 min, and 60 min). At $65^{\circ}C$ incubation, the difference was clearly observed after 40 min with 10 ng and100 ng, but with a 60-min incubation period, the minimum DNA needed was 0.1 ng. However, there was little difference in fluorescence among all DNA amounts tested with 20 or 30 min incubations.

Analysis of QTLs Related to Resistance to Brown Planthopper in Rice (DH 집단을 이용한 벼멸구 저항성 연관 QTLs 분석)

  • Kim, Suk-Man;Qin, Yang;Sohn, Jae-Keun
    • Korean Journal of Breeding Science
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    • v.41 no.3
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    • pp.236-243
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    • 2009
  • This study was conducted to develop a japonica-type rice cultivar with brown planthopper (BPH) resistance using DNA markers. A doubled haploid (DH) population consisting of 120 pure-lines was established by anther culture of $F_1$ hybrids between 'Samgang', a Tongil type BPH resistance cultivar, and 'Nagdong', a japonica cultivar. To determine the map position of genes responsible for BPH resistance in rice, a genetic map was constructed based on 120 DH lines. A total of 162 molecular markers were classified into 12 linkage groups, covering 1,884 Kosami centimorgan (cM) with an average of 11.6 cM. Five QTLs (qBPR3, qBPR6, qBPR7, qBPR8, and qBPR12) associated with BPH resistance were identified and mapped on chromosomes 3, 6, 7, 8, and 12, respectively, using the genetic map constructed in this study. To analyze the relationship between BPH resistance and agronomic traits, a total of eight QTLs related to the agronomic traits were detected on 12 rice chromosomes. In an analysis of relationships, three QTLs (qBPR3, qBPR7, and qBPR8) showed a linkage with tested agronomic traits. A QTL (qBPR3) located on chromosome 3 (RM282-3023) was closely linked to culm length (qCL3). The QTL (qBPR8) for BPH resistance on the short arm of chromosome 8 also overlapped the region detected in culm length (qCL8).

Repellent and Insecticidal Activity of Sequential Extracting Fractions Obtained from BPH-Resistant Rice Varieties against Brown Planthopper (Nilaparvata lugens) (벼멸구 저항성벼 품종 추출분획물의 기피 및 살충 활성)

  • Kim, Sung-Eun;Kim, Young-Doo;Kim, Bo-Kyoung;Ko, Jae-Kwon;Chun, Jae-Chul
    • The Korean Journal of Pesticide Science
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    • v.10 no.2
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    • pp.124-130
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    • 2006
  • Rice plant extracts of brown planthopper (BPH) resistant rice varieties, Jangseongbyeo (JSB) and Hwacheongbyeo (HCB) at different growth stages (seedling, tillering, heading and ripening) were sequentially fractioned using hexane, ethyl ether, ethyl acetate, butanol, and distilled water. The extracts were applied to BPH susceptible rice variety, Dongjjnbyeo (DJB), to investigate the insecticidal and repellent effects against BPH. BPH insecticidal effects were not clearly observed with almost all of the extract fractions obtained from both JSB and HCB varieties for 12 h, whereas the ethyl ether and hexane extract fractions showed about 10 to 30% of BPH mortality in 24 to 48 h of application periods. An effective BPH repellent activity was found with the applications of ethyl ether extract fractions obtained from JSB variety. The extract fractions obtained from HCB variety did not show any different repellence among the various fractions. The BPH repellent effects of the extract fractions obtained at different growth stages of either JSB or HCB varieties did not show any correlations. The effect of ethyl ether fraction on BPH repellent was continually increased by 30 h after treatment and thereafter decreased. In addition, the first sub-fraction separated by a flash column chromatography eluted with chloroform:methanol (9:1, v/v) from the BPH effective ethyl ether faction in JSB variety might be meaningful to repel BPH from BPH susceptible target rice plants. The results indicated that the ethyl ether fraction obtained from JSB was higher in repellent activity than in insecticidal activity, and suggesting that there might be specific substance(s) in the first sub-fraction (sF1) of the ethyl ether fraction in JSB that could provide repellent activity against BPH.

Effectiveness of egg-nymphal predation by a mirid bug, Cyrtorhinus lividipennis REUTER, for control of the brown planthopper (벼멸구(Nilaparrata lugens STAL)의 란 및 유추에 대한 장님 노린재 일종(Cyrtorhinus lividipennis REUTER)의 포식효과)

  • Bae S. H.;Pathak M. D.
    • Korean journal of applied entomology
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    • v.5_6
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    • pp.55-58
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    • 1968
  • 본시험은 1966년 필리핀에 있는 국제수도연구소에서 벼멸구의 생태에 관한 시험을 위하여 벼멸구를 대량으로 집단접종사육하여 오딘 중 cage내 갑자기 벼멸구의 밀도가 줄고 장님노린재가 무수히 번식되고 있는 것을 관찰한 후 Hawaii에 있는 Bishop Museum, Dr. ASHLOCK 씨에이 장님노린재에 대한 동정을 의뢰함과 동시에 벼멸구에 대한 포식성을 조사한 결과 벼멸구의 포식식능력은 장님노린재를 산난된 도엽에 접종하였을 때 5일후 포식난율이 $69.1\%$인대 대하여 벼멸구 고충에 대하여서는 접종 1일 후$61.4\%$, 2일 후 $76.2\%$, 3일 후 $91.0\%$ 4일 후 $93.3\%$의 포식률로서 매우 놀라운 포식능력을 보여주었다. 이 장님노린채는 벼멸구뿐만 아니라 다른 멸구류의 밀도를 제압할 수 있기 때문에 단장조건하에서는 벼멸구류의 피해를 미연에 경감시킬 수 있는 유리한 천적이라고 생각되며, 금후 이의 실제적인 이용면에 대한 연구가 기대된다.

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A Systematic Application of Insecticides to manage Early Season Insect Pests and Migratory Planthoppers on Rice (본논초기 해충군과 비래성 멸구류의 밀도억제를 위한 살충제의 체계적 처리에 관한 연구)

  • 배윤환;이준호;현재선
    • Korean journal of applied entomology
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    • v.33 no.4
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    • pp.270-280
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    • 1994
  • This study was conducted to establish a nce insect pest control system, ~ es.ys tematic application of insecticides using carbohran and buproiezin, in Korea. The effects oi various dates of application and rates of buprofezin (25% WP) after carbofuran (3G) soil incorpombon in late May on the pop~~lation densities of the brown planthoppa (BPH) immigrating in July were investigated Appropriate application tune of buprofezin for the BPH that had evaded insecticidal effect of 5011 incorporated carbofuran was late July-early August. Application rate of buprofezln at 7.0g a.i/lOa was enough to suppress the BPH density. Buprofezin treatment after carbofumn soil incorporation could also suppress the whlte backed planthopper population but did not affect the densities oi the paddy rice spiders. Considering the charactenstics of occurring patterns of the nce insect pests in Korea. buprofezin treatment m late July or early August after carbofuran soil incorporation in late May can be a useful application system of ir~sectic~deins controlling early season Insect pests and migmtoly planthoppers on rice.

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Development of RAPD Marker Related to Brown Planthopper Resistance Gene Derived from Rice Cultivar, Cheongcheongbyeo (청청벼에서 유래한 벼멸구 저항성관련 RAPD Marker의 개발)

  • Seo Ji-Hun;Kim Kyung-Min;Kim Suk-Man;Sohn Jea-Keun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.6
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    • pp.453-456
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    • 2005
  • This study was carried out to select DNA markers closely linked to brown planthopper (BPH) resistance gene originated from a rice cultivar 'Cheongcheong­byeo'. For the mapping of resistant gene to BPH, a doubled-haploid (DH) population was developed by anther culture of $F_1$ plants from a cross 'Cheongcheong­byeo/Nagdongbyeo'. In BPH bioassay and marker screen­ing for the DH population, the segregation of resistant and susceptible plants to BPH fitted to a 1:1 ratio. A total of 310 RAPDs of 520 markers showed polymorphism in parental survey using 'Cheongcheongbyeo' and 'Nag­dongbyeo'. In the analysis of relationship between BPH resistance and marker pattern for 40 DH lines, the OPE16 produced a specific dominant fragment, 700 bp, which was closely linked with BPH resistance gene of 'Cheong­cheongbyeo'. Based on the linkage analysis using 7 markers, BPH resistance of 'Cheongcheongbyeo' was mapped on chromosome 12, which was closely linked with $OPE16_{700}$ at a distance of 4.6 cM.

Some Aspects of Population Dynamics of Rice Leafhoppers in Korea (한국에 있어서 벼멸구$\cdot$매미충의 개체군동태에 관하여)

  • Hokyo Nobuhiko;Lee Moon Hong;Park Joong Soo
    • Korean journal of applied entomology
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    • v.15 no.3
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    • pp.111-126
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    • 1976
  • Regional differences in economic importance of the four species of rice leafhoppers, viz., Laodelphax striatellus, Nephotettix cincticeps, Nilaparvata Iugens and Sogatella furcifera, were analyzed and discussed based on population surveys conducted in Suweon and in southern and south-western coastal areas. The economic importance of L. striatellus and N. cincticeps, which are found throughout the year in Korea, seems to be less in middle regions. This is believed due to effective natural control through severe winter climate, cultural practices in rice and barley growing, and natural enemies such as spiders. However, the economic importance of these two species is significantly greater in southern regions with a less severe winter climate, and where cultural practices in rice and barley favour synchronization between the life cycles of insects and the seasonal succession of host plants. with regard to N. lugens and S. furcifera, which cannot overwinter in Korea, initial populations are the result of long distance migration across the East China Sea. Weather elements related to their migration result in more abundant immigrants of these two species in southern than in middle regions. Thus, different policies are needed for the integrated control of rice leafhoppers in middle and southern Korea.

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