• Title, Summary, Keyword: 방사선 방어효과

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Radioprotective Effects of Propolis on the Mouse Testis Exposed to X-ray. (프로폴리스가 X-선에 노출된 마우스 정소에 미치는 방사선 방어 효과)

  • Ji, Tae-Jung;Kim, Jong-Sik;Jeong, Hyung-Jin;Seo, Eul-Won
    • Journal of Life Science
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    • v.17 no.5
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    • pp.664-670
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    • 2007
  • The propolis is natural product produced by honeybees and is known to have many biologically useful properties such as anti-microbial, anti-oxidative and anti-tumorigenic activity. However, its radio-protective property has not been well studied. To investigate radio-protective effect of propolis on mouse testis, mice were supplemented with propolis after 5 Gy irradiation. The histological changes of testis were detected by TEM. The results indicate that propolis may protect tissue deformation which is induced by 5 Gy of ionizing radiation. Furthermore, to elucidate the potential molecular mechanisms involved in radio-protective property of propolis, we performed microarray experiments using oligo DNA microarray. We found 65 up-regulated genes and 224 down-regulated genes, whose expression levels were affected more than 2-fold by propolis treatment in mice irradiated at 5 Gy. We confirmed microarray data with reverse transcription-PCR using gene specific primers. The results of RT-PCR are highly correlated with those of microarray. These results may help understanding molecular mechanisms of radioprotective effects by propolis in mouse model.

Effect of Cobaltous Chloride on the Repair of UV-induced DNA Damage (UV에 의해 손상된 DNA 회복에 미치는 cobaltous chloride의 효과)

  • Kim, Kug-Chan;Kim, Yung-Jin;Lee, Kang-Suk
    • Journal of Radiation Protection and Research
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    • v.20 no.2
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    • pp.71-78
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    • 1995
  • To develop methods to reduce radiation risk and apply such knowledge to improvement of radiation protection, the effects of cobaltous chloride known as bioantimutagen on the function of E. coli RecA protein involved in the repair of DNA damage were examined. The results demonstrated two distinct effects of cobaltous chloride on the RecA protein function necessary for the strand exchange reaction. Cobaltous chloride enhanced the ability of RecA protein to displace SSB protein from single-stranded DNA and the duplex DNA-dependent ATPase activity. RecA protein was preferentially bound with UV-irradiated supercoiled DNA as compared with nonirradiated DNA The binding of RecA protein to UV-irradiated supercoiled DNA was enhanced in a dose-dependent manner. It is likely that studies on the factors affecting repair efficiency and the DNA repair proteins may provide information on the repair of ionizing radiation-induced DNA damage and the mechanism for DNA radioprotection.

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Change of the Radiation-induced NO(nitric oxide) in Mice with Treatment by Algin-oligosaccharide (알긴산올리고당 처치 마우스에서 방사선 유도 산화질소의 변화)

  • Jang, Woo-Young;Choi, Seong-Kwan;Dong, Kyung-Rae
    • The Journal of the Korea Contents Association
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    • v.9 no.7
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    • pp.211-217
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    • 2009
  • In order to find out the radioprotective effect of algin-oligosaccharide, this study, with a mouse of which whole body irradiated by 3 Gy radiation once, measured nitric oxide. In nitric oxide test for observing the reaction of cell inflammation, nitric oxide showed decreased in the irradiation control group, while 3 day's treatment group with algin-oligosaccharide before or after irradiation indicated higher than the irradiation control group, especially showed big difference in 3 day's treatment group before irradiation (P<0.001). Consequently, this study inquired into the fact that algin-oligosaccharide with superior antioxidant activity performed radiation protection by increasing promotion of nitric oxide generation and confirmed that natural product with less chemical toxicity was able to be applied as radioprotector.

Representative Parameter of Immunostimulatory Ginseng Polysaccharide to Predict Radioprotection (방사선 방어효과 예측 가능한 면역증강 인삼 다당체의 활성인자)

  • Son, Hyeog-Jin;Shim, Ji-Young;Ahn, Ji-Yeon;Yun, Yeon-Sook;Song, Jie-Young
    • Journal of Radiation Protection and Research
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    • v.33 no.3
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    • pp.99-104
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    • 2008
  • According to the increase in the use of radiotherapy to cancer patients, many approaches have been tried to develop new agents for the protection of surrounding normal tissues. However, it is still few applied in the clinic as a radioprotector. We aim to find a representative parameter for radioprotection to easily predict the activity of in vivo experiment from the results of in vitro screening. The polysaccharide extracted from Panax ginseng was used in this study because the immunostimulator has been regarded as one of the radioprotective agent category and was already reported having a promising radioprotective activity through the increase of hematopoietic cells and the production of several cytokines. Mitogenic activity, AK cells activity and nitric oxide production were monitored for the in vitro immunological assay, and endogenous colony-forming unit (e-CFU) was measured as in vivo radioprotective parameter. The immunological activity was increased by the galactose contents of ginseng polysaccharide dependently. The result of this study suggests that mitogenic activity of splenocytes demonstrated a good correlation with in vivo radioprotective effect, and may be used as a representative parameter to screen the candidates for radioprotector.

Radiation-Induced IL(interleukin)-6 in Mice with Algin-Oligosaccharide Treatment (알긴산올리고당 처치 마우스의 방사선 유도 IL-6)

  • Choi, Seong-Kwan;Ji, Youn-Sang
    • Journal of radiological science and technology
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    • v.33 no.4
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    • pp.321-326
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    • 2010
  • To examine the radioprotective effect of algin-oligosaccharide(AOS), radiation-induced IL(interleukin)-6 in mice treated with 3 Gy whole body irradiation once were examined. In the measurement of irradiation-induced IL-6, in comparison with the irradiation control group, in both small intestine and liver tissues of the group treated with algin-oligosaccharide for 7 days prior to irradiation, was suppressed IL-6 synthesis(p < 0.001). It is considered that the protection against radiation hazard by antioxydative reaction of algin-oligosaccharide results in down control of IL-6 value in experimental groups treated with algin-oligosaccharide. In conclusion, through our study, the fact that algin-oligosaccharide has irradiation protection effects was elucidated, and simultaneously, the possibility of the use of a natural product without chemical toxicity as an irradiation protection agent was confirmed.

Radioprotection of Alliin in Oogenesis Cells of a White Rat (흰쥐 난자형성 세포의 알리인 방어효과)

  • JI, Tae-Jeong
    • Journal of radiological science and technology
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    • v.41 no.5
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    • pp.471-478
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    • 2018
  • Oogenesis process of ovary produces a lot of undifferentiated cells. Especially, the radiation exposure of early immature cells in the process of growth to oocyte causes serious disabilities. This study examined the radiation damage mechanism of undifferentiated cells and organelles in oogenesis process, and the radioprotection after injection of alliin. The ultrastructure after 7Gy X-ray irradiation on the white rat was observed in the experiment. The results is as follows. It was observed that the nucleus membrane of an oogonium was damaged and vacuolated in the several parts after 15 days of irradiation. The damage of mitochondria membrane and flow in cytoplasm after 20 and 30 days was found in the oogonium. After 40 days observation, peroxidation of fat droplets was found and organelles were tangled each other in ovary tissue. The partial damage of nuclear membrane in oogonium past 15 days after injection of alliin was found, but decreased remarkably. Mitochondria, Golgi body, and rough endoplasmic reticulum were also clearly observed, therefore, radioprotection effects in alliin was confirmed partially.

An Experimental Study of Radioprotective Effect of Ginseng Alkaloid Fraction on Cellular Damage (방사선 세포 손상에 대한 인삼 Alkaloid 분획의 보호 효과에 관한 실험적 연구)

  • Yoo, Seong-Yul;Cho, Chul-Koo;Kim, Mi-Sook;Yoo, Hyung-Jun;Kim, Seong-Ho;Kim, Tae-Hwan
    • Journal of Radiation Protection and Research
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    • v.22 no.3
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    • pp.195-205
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    • 1997
  • This paper is to assess the effect of Adaptagen as a radioprotector in which main component is alkaloid fraction of ginseng. Evaluation was made in vitro and in vivo study with NIGP(S) mouse by the measurement of regeneration of jejunal crypt cell and micronucleus assay to analyze radioprotective effect of ginseng alkaloid fraction in comparison with that of water fraction after whole body irradiation. The results were as follows, 1. The degree of radiation damage of mouse jejunal crypt cell was diminished in both of alkaloid and water fraction groups compared to control group but more in alkaloid fraction group than water fraction group. Regeneration of mouse jejunal crypt cell was higher both in alkaloid and water fraction groups than control group. 3. In vitro study, frequency of micronucleus was diminished in tendency for the treated groups than control group but statistically insignificant. 4. In vitro study, frequency of micronucleus was diminished in both alkaloid and water fraction groups compared to control group but more in alkaloid fraction group than water fraction group.

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