• Title, Summary, Keyword: 방사선돌연변이

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Characteristics of Antifungal Bacterium, Bacillus subtilis YS1 and It′s Mutant Induced by Gamma Radiation (온천수로부터 분리한 항진균세균의 특성 및 감마선$(Co^{60})$ 조사를 이용한 돌연변이체 유기)

  • 이영근;김재성;송인근;정혜영;장화형
    • Korean Journal of Microbiology
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    • v.37 no.4
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    • pp.305-311
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    • 2001
  • Antifungal bacterium, Bacillus subtilis YS1 was isolated from Yusong hot spring showed broad antifungal spectrum against 12 kinds of plant pathogenic fungi and Candida albicans, animal pathogen. From the gamma($Co^{60}$) radiation sensitivity test, $D_10$ value was 2.08 kGy and it survived above 20 kGy of radiation dose. Several mutants were induced by gamma radiation. Among them, YS1-1009 mutant showed resistance against tebuconazole of herbicide, increased activity against Botryoshaeria dothidea and ligninase activity. YS67 mutant was antifungal deficient auxotrophic mutants(trp-pro-or arg-ura-). From this results, it suggested that gamma irradiation could be useful method for mutant induction.

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Microarray Analysis of Radiation Related Gene Expression in Mutants of Bacillus lentimorbus WJ5 Induced by Gamma Radiation (Bacillus lentimorbus WJ5의 감마선유도 돌연변이체들에서 공통으로 발현되는 방사선 관련 유전자의 microarray 분석)

  • Lee Young-Keun;Chang Hwa-Hyoung;Jang Yu-Sin;Huh Jae-Ho;Hyung Seok-Won;Chung Hye-Young
    • Korean Journal of Environmental Biology
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    • v.22 no.3
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    • pp.472-477
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    • 2004
  • To study the radiation related gene expression in mutants of Bacillus lentimorbus WJ5 induced by gamm radiation, the simultaneous gene expression was analyzed by DNA micro array. We constructed DNA chips including two thousand randomly digested genome spots of B. lentimorbus WJ5 and compared its quantitative aspect with seven mutants induced by gamma radiation $(^{60}/Co)$. From the cluster analysis of gene expression pattern, totally 408 genes were expressed and 27 genes were significantly upregulated by the gamma radiation in all mutants. Especially, genes involved in repair (mutL, mutM), energy metabolism (acsA, sdhB, pgk, yhjB, citB), protease (npr), and reduction response to oxidative stress (HMM) were simultaneously upregulated. It seems that the induction of the direct and/or indirect repair related genes in mutants induced by gamma radiation could be remarkably different from the adaptive responses against acute exposure to radiation.

사람 T-임파구의 HPRT유전자에서 방사선 및 환경독성물질에 노출에 의한 돌연변이 빈도의 변화

  • 윤병수;조명행;이영순;김인규;이강석
    • Proceedings of the Korean Nuclear Society Conference
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    • pp.40-45
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    • 1996
  • 사람 T-임파구에서 방사선 독성물질인 감마선과 화학적 독성물질인 PCP(pentachlorophenol)의 돌연변이 효과를 hprt (hypoxanthine phosphoribosyl transferase) 유전자의 돌연변이빈도로써 측정하였다. 감마선은 $^{137}$Cs원을 사용하여, 0 - 300 rads의 양으로 세포의 초기배양 시기에 조사하였으며, PCP는 역시 세포의 초기배양시 최종농도 0-100 ppm으로 24시간 투여 하였다. 양 돌연변이원은 hprt유전자를 돌연변이 시키어, 300rads의 조사는 대조군에 비하여 약 7.5배의 돌연변이빈도의 증가를 나타내었고, PCP 500 ppm의 처리는 대조군에 비하여 약 5배의 돌연변이빈도 증가를 나타내었다. 본 실험에 사용된 상이한 두 돌연변이원은 모두 비교적 정확한 용량-반응 관계를 보였으나, 환경독성물질들의 혼합효과에서 돌연변이원을 정성하기위하여 개선된 T-cell hprt clonal assay, 즉 reverse transcriptase/polymerase chain reaction 및 direct sequencing에 의한 mutational spectrum의 적용이 요구되었다.

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Effect of Photoperiod on Radiation-Induced Pink Mutations in Tradescantia Stamen Hairs (자주달개비 수술털에서 방사선에 의해 유발되는 분홍돌연변이에 대한 광주기의 영향)

  • 김원록;김진규
    • Korean Journal of Environmental Biology
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    • v.17 no.3
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    • pp.331-335
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    • 1999
  • The present study was carried out to investigate the combined effect of radiation and photoperiod (PP) regimes on Tradescantia 4430 somatic cell mutations. Potted plants were irradiated with 0.3, 0.5 and 1.0 Gy of gamma radiation from 60Co source. The plants irradiated only with gamma radiation were used as control group (CT). The somatic cell mutation rate in 0.5 Gy irradiated CT and PP20 group started to increase on the 6th day and reached a maximum value on the l0th day and 9th day after irradiation while the rate in the experimental group under 4 hours of photoperiod a day (PP4) started to increase on the l0th day and reached a maximal value on the 16th day post-irradiation. The slope of dose-response curve in CT was 5.99 ($r^2$=0.99), while it was 6.93 ($r^2$=0.98) in PP20 and 11.74 ($r^2$=0.99) in PP4, respectively. The biological efficacy of radiation in the induction of pink mutation increased by 15.7% in PP20 and 95.9 % in PP4, respectively. It is suggested that photoperiod regimes unfavorable to the plant have an additive effect on radiation-induced mutations and a delaying or inhibiting effect on cell damage repair, as well.

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Comparison of Mutant Frequencies Induced by ${\gamma}$-radiation and Pentachlorophenol at hprt Locus in Human T-lymphocytes(I) (인체 T-림프구 hprt 유전자에서 방사선 및 pentachlorophenol에 의한 돌연변이 빈도의 비교(I))

  • Kim, In-Gyu;Park, Seon-Young;Yoon, Byung-Su;Cho, Myung-Haing;Lee, Yong-Soon
    • Journal of Radiation Protection and Research
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    • v.22 no.1
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    • pp.15-21
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    • 1997
  • In vitro somatic mutation induced by ${\gamma}$-radiation and pentachlorophenol(PCP) which is representative of chemical pollutant was measured at the hypoxanthine-guanine phosphoribosyl transferase(hprt) locus in human T- lymphocytes by a cell cloning assay. Mutant cells were selected by their ability to form a clone in the presence of purine analogue 6-thioguanine. The mutant frequencies by ${\gamma}$-irradiation to a dose of 1.0 Gy, 2.0 Gy and 3.0 Gy were 40%, 400% and 750% higher than those in controls. Significant changes were not observed in mutant frequencies in the 0.2 Gy and 0.5 Gy irradiated groups. When the doses of PCP were 15 ppm, 25 ppm and 50 ppm, the mutant frequencies increased by 30%, 90% and 520%, respectively. No changes were observed in the 10 ppm treated group. Similar types of dose-response relationship were shown in the two different mutagens. Reverse transcriptase/polymerase chain reaction technique(RT/PCR) was needed for the mutation spectrum to discriminate combined exposure to radiation and chemicals.

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Effects of Combined Irradiation of Neutrons and ${\gamma}$-Rays on the Pink Mutation Frequencies in Tradescantia (중성자와 감마선 중복 조사가 자주달재비 분홍돌연변이율에 미치는 영향)

  • Kim, Jin-Kyu;Lee, Young-Keun;Kim, Jae-Sung;Shin, Hae-Shick;Hyun, Soung-Hee
    • Journal of Radiation Protection and Research
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    • v.25 no.2
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    • pp.67-73
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    • 2000
  • The effects of combined irradiation of neutrons and ${\gamma}$-rays were analysed by means of Tradescantia stamen hair (TSH) assay. Potted plants were irradiated with $0{\sim}2$ Gy of ${\gamma}$-rays. For the combined treatments, the plants were irradiated with neutrons from $^{252}Cf$ 24 hours before or after ${\gamma}$-irradiation. The slopes of dose-response curves were 5.98, 6.17 and 7.48, in ${\gamma}$-rays, ${\gamma}$-rays+neutrons, and neutrons+${\gamma}$ -rays irradiated groups, respectively. The biological efficacy of radiations in the induction of pink mutations increased by 25% in neutrons+${\gamma}$-rays irradiated group, while it increased by 12% in ${\gamma}$-rays+neutrons irradiated group. The combined irradiation with two kinds of radiations results in the different efficacy of radiation depending on the order of irradiations, which is of great importance in the related fields such as sterilization and radiotherapy.

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