Damage detection methods using structural tests can be divided into two methods, i.e., static and dynamic. The static methods which use the stiffness properties of the structure are simpler than the dynamic methods. However, static approaches are very sensitive to the displacement measurement noises and modeling errors. The dynamic methods also have limitations in acquiring the natural frequencies and mode shapes of the high frequencies. In this study, a method for the structural damage assessment using sensitivity matrices is developed, in which the drawbacks of the static and dynamic methods can be compensated. Based on the measurement data for the static displacements and dynamic modal properties, the damage locations and the degree of damage are determined using the presented sensitivity matrix method. The efficiency of the proposed method has been examined through numerical simulation studies on truss type structures.