DOI QR코드

DOI QR Code

Comparison of Lignocellulose degradation properties of Lentinula edodes varieties

표고(Lentinula edodes) 품종별 목질계 섬유소 분해효소 특성 비교

  • Jeong, Sang-Wook (Jangheung Research Institute for Mushroom Industry) ;
  • Jang, Eun-Kyoung (Jangheung Research Institute for Mushroom Industry) ;
  • Choi, Seul-Ki (Jangheung Research Institute for Mushroom Industry) ;
  • Seo, Kyoung-Sun (Jangheung Research Institute for Mushroom Industry) ;
  • Jeong, Hee-Gyeong (Jangheung Research Institute for Mushroom Industry) ;
  • Lee, Won-Ho (Forest Mushroom Research Center) ;
  • Ban, Seung-Eon (Jangheung Research Institute for Mushroom Industry)
  • 정상욱 (장흥군버섯산업연구원) ;
  • 장은경 (장흥군버섯산업연구원) ;
  • 최슬기 (장흥군버섯산업연구원) ;
  • 서경순 (장흥군버섯산업연구원) ;
  • 정희경 (장흥군버섯산업연구원) ;
  • 이원호 (산림버섯연구센터) ;
  • 반승언 (장흥군버섯산업연구원)
  • Received : 2021.11.30
  • Accepted : 2022.03.23
  • Published : 2022.03.31

Abstract

In this study, five different Lentinula edodes cultivar (Chamaram, Sanbaekhyang, Sanjo 713ho, Sanjo 715ho, Sanjo 718ho) were evaluated for their ability to decolorize Remazol Brilliant Blue R (RBBR) in MEB medium, respectively. Chamaram and Sanjo 713ho decolorized RBBR rapidly in MEB medium within 3 and 5 days. The activities of manganese peroxidase (MnP) and laccase were determined on the MEB medium with and without lignin. Sanjo 713ho resulted the highest ligninolytic enzyme activities on incubation day 1, indicating of 1,213 U/mg of MnP activity and 1,421 U/mg of laccase activity.

본 연구에서는 톱밥재배용 표고(Lentinula edodes) 품종에 대한 목질계 섬유소의 분해특성을 분석하였다. 국산 표고 품종 참아람, 산백향, 산조 713호, 산조 715호, 산조 718호를 대상으로 RBBR (Remazol Brilliant Blue R) 탈색능, 목질계 섬유소 분해 효소인 MnP와 laccase의 활성을 비교 분석하였다. 5개 품종의 탈색능과 효소활성은 품종별로 다른 양상을 보였다. 리그닌의 첨가에 따른 RBBR 탈색능을 조사한 결과, 참아람과 산조 713호가 각각 배양 3일과 5일째부터 우수한 분해능을 보였다. 섬유소 분해 효소인 MnP와 laccase의 활성은 산조 713호가 배양 2일째 MnP 활성이 1,213 U/mg, laccase 활성이 1,421 U/mg, 다음으로 참아람이 배양 7일째 MnP 활성이 1,921 U/mg, laccase 활성이 2,123 U/mg으로 최고 활성을 나타냈다. 이러한 결과는 표고 육종에 있어 리그닌 분해능이 우수한 균주의 선발이 가능함에 따라 교배균주의 육종 모본 활용에 효율적일 것으로 판단된다.

Keywords

Acknowledgement

본 연구는 농림축산식품부의 재원으로 농림식품기술기획평가원의 Golden Seed 프로젝트 사업의 지원을 받아 연구되었음(213007-05-5-WTH31).

References

  1. Baldrian J, Savoie, M Foulongne-oriol, M Largeteau, M Barroso G. 2011. Production of lignocellulolytic enzymes by mushrooms. In Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products. 1: 334-338.
  2. Choi BA. 2021. Statistical yearbook of forestry. Korea Forest Service.
  3. Eriksson KE. 1990. Biotechnology in the pulp and paper industry. Wood Science and Technology. 24: 79-101.
  4. Glenn JK, Gold MH. 1983. Decolorization of several polymeric dyes by the lignin-degrading basidiomycete Phanerochaete chrysosporium. Applied and Environmental Microbiology. 45: 1741-1747.
  5. Hong CY, Kim HY, Jang SK, Choi IG. 2013. Screening of Outstanding White Rot Fungi for Biodegradation of Organosolv Lignin by Decolorization of Remazol Brilliant Blue R and Ligninolytic Enzymes Systems. Wood Sci. & Tech. 41: 19-32.
  6. Kitamoto Y. 2006. Current progress in breeding of edible and pharmaceutical mushrooms. Mokuzai Gakkaishi. 52: 1-7.
  7. Kim SY, Choi JY, Choi HT. 2017. Decolorization of dyes by a purified laccase from Coprinus comatus. Microbiology. 53: 79-82.
  8. Krcmar P, Kubatova A, Votruba J, Erbanova P, Novotny C, Sasek V. 1999. Degradation of polychlorinated biphenyls by extracellular enzymes of Phanerochaete chrysosporium produced in a perforated plate bioreactor. World Journal of Microbiology and Biotechnology. 15: 269-276.
  9. Lee TS, Yoon KH, Bak WC, Kim JS, Lee JY. 2000. New cultivation technology of oak mushroom. Korea Forest Research Institute pp. 174-175.
  10. Machado KM, Matheus DR, Bononi VL. 2005. Ligninolytic enzymes production and Remazol Brilliant Blue R decolorization by tropical Brazilian basidiomycetes fungi. Brazilian Journal of Microbiology. 36: 246-252.
  11. Mizuno, T. 1995. Shiitake, Lentinula edodes: functional propertiesfor medicinal and food purpose. Food Rev. Int. 11: 7-21.
  12. Pandey KK, Pitman A. 2003. FTIR studies of the changes in wood chemistry following decay by brown-rot and white-rot fungi. International biodeterioration & biodegradation. 52: 151-160.
  13. Park IC, Seok SJ, Kim JS, Yoo JH, Ahn JH. 2015. Analysis of Mycological Characteristics and Lignocellulose Degradation of Gyrodontium sacchari. Mycology. 43: 239-246.
  14. Scarse R. 1995. Cultivating mushrooms-the potential. Mycologist. 9: 18-19.
  15. Schmidt, O. 2006. Wood and tree fungi: biology, damage, protection, and use. Springer Science & Business Media.