Development of Real-time Quantitative PCR Assay based on SYBR Green I and TaqMan Probe for Detection of Apple Viruses

사과 바이러스 검정을 위한 SYBR Green I 및 TaqMan probe 기반의 real-time PCR 검사법 개발

  • Heo, Seong (Department of Horticulture, Kongju National University) ;
  • Chung, Yong Suk (Department of Plant Resources and Environment, Jeju National University)
  • 허성 (공주대학교 산업과학대학 원예학과) ;
  • 정용석 (제주대학교 생명자원과학대학 식물자원환경전공)
  • Received : 2020.06.13
  • Accepted : 2020.09.27
  • Published : 2020.12.01


Virus infections of apples result in lowered commercial qualities such as low sugar content, weakened tree vigor, and malformed fruits. An effective way to control viruses is to produce virus-free plants based on the development of an accurate and sensitive diagnostic method. In this study, real-time PCR assays based on SYBR Green I and TaqMan probes were developed for detecting ASGV, ASPV, and ApMV viruses. These methods can detect and quantify 103 to 1011 RNA copies/μL of each virus separately. Compared with methods with two different dyes, the SYBR Green I-based method was efficient for virus detection as well as for assay using the TaqMan probe. Field tests demonstrated that real-time PCR methods developed in this study were applicable to high-throughput diagnoses for virus research and plant quarantine.