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Development of Real-time Quantitative PCR Assay based on SYBR Green I and TaqMan Probe for Detection of Apple Viruses

사과 바이러스 검정을 위한 SYBR Green I 및 TaqMan probe 기반의 real-time PCR 검사법 개발

  • Heo, Seong (Department of Horticulture, Kongju National University) ;
  • Chung, Yong Suk (Department of Plant Resources and Environment, Jeju National University)
  • 허성 (공주대학교 산업과학대학 원예학과) ;
  • 정용석 (제주대학교 생명자원과학대학 식물자원환경전공)
  • Received : 2020.06.13
  • Accepted : 2020.09.27
  • Published : 2020.12.01

Abstract

Virus infections of apples result in lowered commercial qualities such as low sugar content, weakened tree vigor, and malformed fruits. An effective way to control viruses is to produce virus-free plants based on the development of an accurate and sensitive diagnostic method. In this study, real-time PCR assays based on SYBR Green I and TaqMan probes were developed for detecting ASGV, ASPV, and ApMV viruses. These methods can detect and quantify 103 to 1011 RNA copies/μL of each virus separately. Compared with methods with two different dyes, the SYBR Green I-based method was efficient for virus detection as well as for assay using the TaqMan probe. Field tests demonstrated that real-time PCR methods developed in this study were applicable to high-throughput diagnoses for virus research and plant quarantine.