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Antiserum Preparation of Recombinant Sweet Potato Latent Virus-Lotus (SPLV-Lotus) Coat Protein and Application for Virus-Infected Lotus Plant Detection

  • He, Zhen (School of Horticulture and Plant Protection, Yangzhou University) ;
  • Dong, Tingting (School of Horticulture and Plant Protection, Yangzhou University) ;
  • Chen, Wen (School of Horticulture and Plant Protection, Yangzhou University) ;
  • Wang, Tielin (National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, State Key Laboratory Breeding Base of Dao-di Herbs) ;
  • Gan, Haifeng (School of Horticulture and Plant Protection, Yangzhou University) ;
  • Li, LiangJun (School of Horticulture and Plant Protection, Yangzhou University)
  • 투고 : 2020.03.05
  • 심사 : 2020.11.17
  • 발행 : 2020.12.01

초록

Lotus is one of the most important aquatic vegetables in China. Previously, we detected sweet potato latent virus from lotus (SPLV-lotus) and found that it has highly significant sequence diversity with SPLV-sweet potato isolates (SPLV-sp). Here, we developed serological methods for the detection of SPLV-lotus in Chinese lotus cultivation areas. Based on the high sensitivity of SPLV-lotus coat protein antiserum, rapid, sensitive and large-scale diagnosis methods of enzyme-linked immunosorbent assay (ELISA) and dot blot in lotus planting area were developed. The established ELISA and dot blot diagnostic methods can be used to detect SPLV-lotus from samples successfully. And our results also showed that the SPLV-lotus and sweet potato isolates appeared clearly distinction in serology. Our study provides a high-throughput, sensitive, and rapid diagnostic method based on serology that can detect SPLV on lotus, which is suggested to be included in viral disease management approach due to its good detection level.

참고문헌

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