- Volume 36 Issue 6
DOI QR Code
Antiserum Preparation of Recombinant Sweet Potato Latent Virus-Lotus (SPLV-Lotus) Coat Protein and Application for Virus-Infected Lotus Plant Detection
- He, Zhen (School of Horticulture and Plant Protection, Yangzhou University) ;
- Dong, Tingting (School of Horticulture and Plant Protection, Yangzhou University) ;
- Chen, Wen (School of Horticulture and Plant Protection, Yangzhou University) ;
- Wang, Tielin (National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, State Key Laboratory Breeding Base of Dao-di Herbs) ;
- Gan, Haifeng (School of Horticulture and Plant Protection, Yangzhou University) ;
- Li, LiangJun (School of Horticulture and Plant Protection, Yangzhou University)
- Received : 2020.03.05
- Accepted : 2020.11.17
- Published : 2020.12.01
Lotus is one of the most important aquatic vegetables in China. Previously, we detected sweet potato latent virus from lotus (SPLV-lotus) and found that it has highly significant sequence diversity with SPLV-sweet potato isolates (SPLV-sp). Here, we developed serological methods for the detection of SPLV-lotus in Chinese lotus cultivation areas. Based on the high sensitivity of SPLV-lotus coat protein antiserum, rapid, sensitive and large-scale diagnosis methods of enzyme-linked immunosorbent assay (ELISA) and dot blot in lotus planting area were developed. The established ELISA and dot blot diagnostic methods can be used to detect SPLV-lotus from samples successfully. And our results also showed that the SPLV-lotus and sweet potato isolates appeared clearly distinction in serology. Our study provides a high-throughput, sensitive, and rapid diagnostic method based on serology that can detect SPLV on lotus, which is suggested to be included in viral disease management approach due to its good detection level.
- Bhimji, A., Zaragoza, A. A., Live, L. S. and Kelley, S. O. 2013. Electrochemical enzyme-linked immunosorbent assay featuring proximal reagent generation: detection of human immunodeficiency virus antibodies in clinical samples. Anal. Chem. 85:6813-6819. https://doi.org/10.1021/ac4009429
- Boonham, N., Kreuze, J., Winter, S., van der Vlugt, R., Bergervoet, J., Tomlinson, J. and Mumford, R. 2014. Methods in virus diagnostics: from ELISA to next generation sequencing. Virus Res. 186:20-31. https://doi.org/10.1016/j.virusres.2013.12.007
- Chiang, P. Y. and Luo, Y. Y. 2007. Effects of pressurized cooking on the relationship between the chemical compositions and texture changes of lotus root (Nelumbo nucifera Gaertn.). Food Chem. 105:480-484. https://doi.org/10.1016/j.foodchem.2007.04.003
- Clark, M. F. and Adams, A. N. 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol. 34:475-483. https://doi.org/10.1099/0022-1317-34-3-475
- Engvall, E. and Perlmann, P. 1971. Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin G. Immunochemistry 8:871-874. https://doi.org/10.1016/0019-2791(71)90454-X
- Hammond, J., Jordan, R. L., Larsen, R. C. and Moyer, J. W. 1992. Use of polyclonal antisera and monoclonal antibodies to examine serological relationships among three filamentous viruses of sweetpotato. Phytopathology 82:713-717. https://doi.org/10.1094/Phyto-82-713
- He, Z., Chen, W., Chen, C., Liu, X. and Li, L. 2019. First report of Apple stem grooving virus infecting lotus (Nelumbo nucifera) in China. Plant Dis. 103:782.
- Masuda, J.-I., Ozaki, Y. and Okubo, H. 2007. Rhizome transition to storage organ is under phytochrome control in lotus (Nelumbo nucifera). Planta 226:909-915. https://doi.org/10.1007/s00425-007-0536-9
- Tsai, S. J., Hutchinson, L. J. and Zarkower, A. 1989. Comparison of dot immunobinding assay, enzyme-linked immunosorbent assay and immunodiffusion for serodiagnosis of paratuberculosis. Can. J. Vet. Res. 53:405-410.
- Wang, H., Liu, X., Gan, H., Chen, W., Wu, P., Li, L. and He, Z. 2019. Genomic and biological characterization of a novel strain of Sweet potato latent virus isolated from lotus (Nelumbo nucifera Gaertn.). J. Plant Pathol. 101:1077-1084. https://doi.org/10.1007/s42161-019-00338-y
- Wang, M., Abad, J., Fuentes, S. and Li, R. 2013. Complete genome sequence of the original Taiwanese isolate of Sweet potato latent virus and its relationship to other potyviruses infecting sweet potato. Arch Virol. 159:2189-2192. https://doi.org/10.1007/s00705-014-2008-4
- Wylie, S. J., Adams, M., Chalam, C., Kreuze, J., Lopez-Moya, J. J., Ohshima, K., Praveen, S., Rabenstein, F., Stenger, D., Wang, A., Zerbini, F. M. and ICTV Report Consortium. 2017. ICTV virus taxonomy profile: Potyviridae 98:352-354. https://doi.org/10.1099/jgv.0.000740
- Xie, Y., Jiao, X., Zhou, X., Liu, H., Ni, Y. and Wu, J. 2013. Highly sensitive serological methods for detecting Tomato yellow leaf curl virus in tomato plants and whiteflies. Virol. J. 10:142. https://doi.org/10.1186/1743-422X-10-142
- Yu, H., Cheng, L., Yin, J., Yan, S., Liu, K., Zhang, F., Xu, B. and Li, L. 2013. Structure and physicochemical properties of starches in lotus (Nelumbo nucifera Gaertn.) rhizome. Food Sci. Nutr. 1:273-283. https://doi.org/10.1002/fsn3.37
- Yu, X., Sheng, J. J., Zheng, X. W., Diao, Y., Zheng, X. F., Xie, K. Q., Zhou, M. Q. and Hu, Z. L. 2015. First report of Dasheen mosaic virus infecting lotus (Nelumbo nucifera) in China. Plant Dis. 99:1449.