DOI QR코드

DOI QR Code

Antiserum Preparation of Recombinant Sweet Potato Latent Virus-Lotus (SPLV-Lotus) Coat Protein and Application for Virus-Infected Lotus Plant Detection

  • He, Zhen (School of Horticulture and Plant Protection, Yangzhou University) ;
  • Dong, Tingting (School of Horticulture and Plant Protection, Yangzhou University) ;
  • Chen, Wen (School of Horticulture and Plant Protection, Yangzhou University) ;
  • Wang, Tielin (National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, State Key Laboratory Breeding Base of Dao-di Herbs) ;
  • Gan, Haifeng (School of Horticulture and Plant Protection, Yangzhou University) ;
  • Li, LiangJun (School of Horticulture and Plant Protection, Yangzhou University)
  • Received : 2020.03.05
  • Accepted : 2020.11.17
  • Published : 2020.12.01

Abstract

Lotus is one of the most important aquatic vegetables in China. Previously, we detected sweet potato latent virus from lotus (SPLV-lotus) and found that it has highly significant sequence diversity with SPLV-sweet potato isolates (SPLV-sp). Here, we developed serological methods for the detection of SPLV-lotus in Chinese lotus cultivation areas. Based on the high sensitivity of SPLV-lotus coat protein antiserum, rapid, sensitive and large-scale diagnosis methods of enzyme-linked immunosorbent assay (ELISA) and dot blot in lotus planting area were developed. The established ELISA and dot blot diagnostic methods can be used to detect SPLV-lotus from samples successfully. And our results also showed that the SPLV-lotus and sweet potato isolates appeared clearly distinction in serology. Our study provides a high-throughput, sensitive, and rapid diagnostic method based on serology that can detect SPLV on lotus, which is suggested to be included in viral disease management approach due to its good detection level.