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Genetic Mapping of a Resistance Locus to Phytophthora sojae in the Korean Soybean Cultivar Daewon

  • Jang, Ik-Hyun (Department of Crop Science, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kang, In Jeong (Department of Central Area Crop Science, National Institute of Crop Science) ;
  • Kim, Ji-Min (Department of Crop Science and Biotechnology, College of Bioresource Science, Dankook University) ;
  • Kang, Sung-Taeg (Department of Crop Science and Biotechnology, College of Bioresource Science, Dankook University) ;
  • Jang, Young Eun (Department of Crop Science, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Lee, Sungwoo (Department of Crop Science, College of Agriculture and Life Sciences, Chungnam National University)
  • Received : 2020.09.07
  • Accepted : 2020.10.26
  • Published : 2020.12.01

Abstract

Phytophthora root and stem rot reduce soybean yields worldwide. The use of R-gene type resistance is currently crucial for protecting soybean production. The present study aimed to identify the genomic location of a gene conferring resistance to Phytophthora sojae isolate 2457 in the recombinant inbred line population developed by a cross of Daepung × Daewon. Singlemarker analysis identified 20 single nucleotide polymorphisms associated with resistance to the P. sojae isolate 2457, which explained ~67% of phenotypic variance. Daewon contributed a resistance allele for the locus. This region is a well-known location for Rps1 and Rps7. The present study is the first, however, to identify an Rps gene locus from a major soybean variety cultivated in South Korea. Linkage analysis also identified a 573 kb region on chromosome 3 with high significance (logarithm of odds = 13.7). This genomic region was not further narrowed down due to lack of recombinants within the interval. Based on the latest soybean genome, ten leucine-rich repeat coding genes and four serine/ threonine protein kinase-coding genes are annotated in this region, which all are well-known types of genes for conferring disease resistance in crops. These genes would be candidates for molecular characterization of the resistance in further studies. The identified R-gene locus would be useful in developing P. sojae resistant varieties in the future. The results of the present study provide foundational knowledge for researchers who are interested in soybean-P. sojae interaction.