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Skin Whitening Effect of Sedum kamtschaticum Fisch. Solvent Fractions

기린초(Sedum kamtschaticum Fisch.) 추출 분획물의 피부 미백효과 연구

  • Yoon, Jihye (Division of Chemistry and Cosmetics, Mokwon University) ;
  • Park, Jihye (Department of Chemistry, The Graduate School of Mokwon University) ;
  • Kim, Bora (Division of Chemistry and Cosmetics, Mokwon University)
  • 윤지혜 (목원대학교 화학.화장품학부) ;
  • 박지혜 (목원대학교 대학원 화학과) ;
  • 김보라 (목원대학교 화학.화장품학부)
  • Received : 2020.10.17
  • Accepted : 2020.10.29
  • Published : 2020.10.31

Abstract

Sedum kamtschaticum Fisch., a native plant of Korea, has been used in Korean traditional medicine in the form of water extract for its capacity to improve blood circulation and for its antioxidant and anti-inflammatory effects. Since previous research suggests that S. kamtschaticum Fisch. has excellent antioxidant and mushroom tyrosinase inhibition activities, in this study, the root and stem parts of S. kamtschaticum Fisch. are extracted in 70% ethanol (SKS, SKR), fractionated with and in order of n-hexane (SSH), ethyl acetate (SSE, SRE), chloroform (SSC, SRC) and water (SSW, SRW) according to the polarity of each solvent, and tested for its applicability as a cosmetic material. According to the total polyphenol, flavonoid contents and DPPH radical scavenging activity of each fraction, the contents and scavenging activity of the root extractions (SKR) were higher than those of the stem extractions (SKS), ethyl acetate fractions (SSE, SRE) being the most effective. In addition, ethyl acetate fractions had the highest tyrosinase inhibition activity and melanin synthesis inhibition activity used on B16F10 melanoma cells, at the concentration of 10 ㎍/mL. HPLC analysis detected a variety of polyphenols including gallic acid and quercetin. This study suggests the potential role of S. kamtschaticum Fisch. as a natural cosmeceutical material.

한국 자생식물인 기린초(Sedum kamtschaticum Fisch.)는 열수 추출 형태로 혈액 순환 개선과 항산화 및 항염증 효과를 내는 한약재로 사용되어왔다. 선행연구를 통해 기린초 여러 부위에서 우수한 항산화 효과를 확인하여, 본 연구에서는 기린초의 줄기(Stem)와 뿌리(Root) 부위를 각각 70% ethanol (SKS, SKR)로 추출한 후, n-hexane (SSH), ethyl acetate (SSE, SRE), chloroform (SSC, SRC) 및 water (SSW, SRW) 순서로 용매 극성별로 분획하여 화장품 소재로서 응용 가능성을 확인하였다. 각 분획물마다 총 폴리페놀 함량, 플라보노이드 함량 및 DPPH 라디칼 소거능을 확인한 결과 뿌리추출물(SKR)이 줄기추출물(SKS)보다 우수한 함량과 효과를 나타냈고, 전체적으로 ethyl acetate 분획물(SSE, SRE)이 가장 효과가 우수했다. 또한, tyrosinase 활성 저해 효과도 ethyl acetate 분획물이 가장 우수하였고, 모든 분획물은 세포독성이 없는 10 ㎍/mL 농도에서 B16F10 멜라노마 세포에 처리했을 때 우수한 멜라닌 생성 저해력을 보였다. 70% ethanol 추출물(SKS, SKR)에 대해 HPLC 분석 시 gallic acid와 quercetin을 포함한 플라보노이드가 검출되었다. 본 연구에서는 기린초의 피부 항산화 효과와 미백효과를 나타내는 기능성화장품 천연소재로서 응용 가능성을 제시한다.

Keywords

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