Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Genotypic characterization of fluoroquinolone-resistant Escherichia coli isolates from edible offal

  • Son, Se Hyun (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University) ;
  • Seo, Kwang Won (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University) ;
  • Kim, Yeong Bin (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University) ;
  • Noh, Eun Bi (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University) ;
  • Lee, Keun-Woo (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University) ;
  • Oh, Tae-Ho (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University) ;
  • Kim, Seung-Joon (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University) ;
  • Song, Jae-Chan (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University) ;
  • Kim, Tae-Wan (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University) ;
  • Lee, Young Ju (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University)
  • Received : 2020.04.09
  • Accepted : 2020.07.10
  • Published : 2020.09.30

Abstract

Edible offal is easily contaminated by Escherichia coli (E. coli) and fluoroquinolone (FQ)-resistant E. coli is considered a serious public health problem, thus, this study investigated the genetic characteristics of FQ-resistant E. coli from edible offal. A total of 22 FQ-resistant E. coli isolates were tested. A double mutation in each gyrA and parC led the highest MIC. Four (18.2%) isolates carried plasmid-mediated quinolone resistance genes. The fimH, eaeA, escV, astA, and iucC genes were confirmed. Seventeen isolates (77.3%) were positive for plasmid replicons. The isolates showed high genetic heterogeneity based on pulsed-field gel electrophoresis patterns.

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