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Effect of Methanol Extract Concentration on the Anti-oxidative Activity and Toxicity of Evodiae Fructus to AGS Cells

오수유의 메탄올 추출 농도에 따른 항산화와 AGS세포에 대한 독성 효과

  • Yang, Ji Yeong (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Byeon, Hwiyong (School of Oriental Medicine and Bio Convergence Sciences, Semyung University) ;
  • Kim, Jin Woo (School of Oriental Medicine and Bio Convergence Sciences, Semyung University) ;
  • Kim, Sa Hyun (Department of Clinical Laboratory Science, Semyung University) ;
  • Lee, Pyeongjae (School of Industrial Bio-pharmaceutical Science, Semyung University)
  • 양지영 (연세대학교 보건과학대학 임상병리학과) ;
  • 변휘용 (세명대학교 한방바이오융합과학부) ;
  • 김진우 (세명대학교 한방바이오융합과학부) ;
  • 김사현 (세명대학교 임상병리학과) ;
  • 이평재 (세명대학교 바이오제약산업학부)
  • Received : 2020.08.03
  • Accepted : 2020.08.26
  • Published : 2020.08.31

Abstract

Evodiae Fructus is the dried unripe fruit of Evodia rutaecarpa, and has traditionally been used for treating stomachache and diarrhea. Evodiamine and rutaecarpine, the major biologically active compounds of Evodiae Fructus, are reported to have anti-oxidative and anti-inflammatory effects, as well as inhibit proliferation and metastasis of various cancer cells. The current study investigates the anti-oxidative and anti-cancer effects of the Evodiae Fructus extract, considering varying concentrations of methanol extraction (40, 80, and 95%). High contents of total phenolic compounds were determined in the order of extracts 80, 95, and 40%. Evaluating contents of the 95, 80, and 40% extracts revealed 36.77, 7.29, and 1.86 ㎍/mg evodiamine, respectively, and 53.02, 17.16, and 3.79 ㎍/mg rutaecarpine, respectively, with the highest content of both compounds obtained in the 95% extract. DPPH radical scavenging activity was observed to be inversely proportional to the contents of total phenolic compounds, with decreasing SC50 values obtained in the order 80, 95, and 40% extract. The 95 and 80% extracts exerted toxicity to AGS gastric cancer cells, but the 40% extract was non-toxic. Evodiamine is a known anti-cancer agent, and could be responsible for the observed toxicity. Cleavage of PARP, and Caspase-3, -7, -8 and -9 was observed in the 95% extract-treated AGS cells, indicating that cell toxicity exerted by the 95% extract could be attributed to apoptosis.

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