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Evaluation of Bioactive Compounds Contents and Biological Activities of Okra Seeds Oils

오크라 씨 기름의 기능성 성분 함량 및 생리활성 평가

  • Seo, Dongyeon (Dept of Food Science and Biotechnology, Kyungsung University) ;
  • Jeon, Ahyeong (Dept of Food Science and Biotechnology, Kyungsung University) ;
  • Shin, Eui-Cheol (Dept of Food Science, Gyeongnam National University of Science and Technology) ;
  • Lee, Junsoo (Division of Food and Animal Sciences, Chungbuk National University) ;
  • Hwang, In Guk (Dept of Agrofood Resources, National Institute of Agricultural Science, Rural Development Administration) ;
  • Kim, Younghwa (School of Food Biotechnology and Nutrition, Kyungsung University)
  • 서동연 (경성대학교 식품생명공학과) ;
  • 전아영 (경성대학교 식품생명공학과) ;
  • 신의철 (경남과학기술대학교 식품과학과) ;
  • 이준수 (충북대학교 식품생명축산학부) ;
  • 황인국 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 김영화 (경성대학교 식품응용공학부)
  • Received : 2020.08.03
  • Accepted : 2020.08.29
  • Published : 2020.08.31

Abstract

This study examined the contents of bioactive compounds and the biological activity of okra seed oil. Okra seed oil consisted mainly of linoleic acid (44.2%). The content of total phytosterols was 2.180 mg/g oil, with β-sitosterol being the highest (1.756 mg/g oil). The vitamin E content was 1.278 mg/g oil; the content of α-tocopherol was higher than γ-tocopherol. The total polyphenol and flavonoid contents were 2.463 mg gallic acid equivalent/g and 1.602 mg cathechin equivalent/g, respectively. The 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid and α-α-diphenyl-β-picrylhydrazyl free radical scavenging activities were 15.297% and 22.265%, respectively, and the reducing power was 4.524 mg gallic acid equivalent/g. The okra seed oil inhibited 77.692% of the α-glucosidase activity. The present study showed that okra seed oil had a considerable amount of phytochemicals and exhibited biological activity. These results suggest that okra seed oil is a potential natural therapeutic for the management of metabolic syndromes.

References

  1. Adelakun OE, Oyelade OJ, Ade-Omowaye BIO, Adeyemi IA, Van de Venter M. 2009. Chemical composition and the antioxidative properties of Nigerian okra seed (Abelmoschus esculentus Moench) flour. Food Chem Toxicol., 47:1123-1126 https://doi.org/10.1016/j.fct.2009.01.036
  2. Alonso-Salces RM, Heberger K, Holland MV, Moreno-Rojas JM, Mariani C, Bellan G, et al. 2009. Multivariate analysis of NMR fingerprint of the unsaponifiable fraction of virgin olive oils for authentication purpose. Food Chem., 118:956-965
  3. Anwar F, Rashid U, Ashraf M, Nadeem M. 2010. Okra (Hibiscus esculentus) seed oil for biodiesel production. Appl Energy., 87:779-785 https://doi.org/10.1016/j.apenergy.2009.09.020
  4. Awad AB, Downie AC, Fink CS. 2000. Inhibition of growth and stimulation of apoptosis by beta-sitosterol treatment of MDA-MB-231 human breast cancer cells in culture. Int J Mol Med., 5:541-545
  5. Bharti SK, Kumar A, Sharma NK, Prakash O, Jaiswal SK, Khrisnan S, et al. 2013. Tocopherol from seeds of Cucurbita pepo against diabetes: Validation by in vivo experiments supported by computational docking. J Formos Med Assoc., 112:676-690 https://doi.org/10.1016/j.jfma.2013.08.003
  6. Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature., 181:1199-1200 https://doi.org/10.1038/1811199a0
  7. Calisir S, Ozcan M, Haciseferogullari H, Yildiz MU. 2005. A study on some physico-chemical properties of Turkey okra (Hibiscus esculenta L.) seeds. J Food Eng., 68:73-78 https://doi.org/10.1016/j.jfoodeng.2004.05.023
  8. Caspary W.F. 1978. Sucrose malabsorption in man after ingest ion of ${\alpha}$-glucosidase hydrolase inhibitior. Lancet., 1:1231-1233
  9. Chanioti S, Tzia C. 2017. Optimization of ultrasound-assisted extraction of oil from olive pomace using response surface technology: Oil recovery, unsaponifiable matter, total phenol content and antioxidant activity. Lebensm Wiss Technol., 79:178-189 https://doi.org/10.1016/j.lwt.2017.01.029
  10. Cho EA, Lee YS. 2014. A study on the classifying quality standard by comparison with phytochemical characteristics of virgin, pure, pomace olive oil. Korean J Food & Nutr., 27(3):339-347 https://doi.org/10.9799/ksfan.2014.27.3.339
  11. Dai J, Muper RJ. 2010. Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules., 15:7313-7352 https://doi.org/10.3390/molecules15107313
  12. Dubey P, Mishra S. 2017. Effect of okra seed in reduction of cholesterol. J Entomol Zool Stud., 5:94-97
  13. Fan S, Zhang Y, Sun Q, Yu L, Li M, Zheng B, et al. 2014. Extract of okra lowers blood glucose and serum lipids in high-fat diet-induced obese C57BL/6 mice. J Nutri Biochem., 25:702-709 https://doi.org/10.1016/j.jnutbio.2014.02.010
  14. Farhoosh R, Tavassoli-Kafrani MH, Sharif A. 2011. Antioxidant activity of the fractions separated from the unsaponifiable matter of bene hull oil. Food Chem., 126:583-589 https://doi.org/10.1016/j.foodchem.2010.11.047
  15. Folin O, Denis W. 1912. On phosphotungstic-phosohomolybdic compounds as colour reagents. J Biol Chem., 12:239-249
  16. Ham H, Oh SK, Lee JS, Choi IS, Jeong HS, Kim IH, et al. 2013. Antioxidant activities and contents of phytochemicals in methanolic extracts of specialty rice cultivars in Korea. Food Sci Biotechnol., 22:631-637 https://doi.org/10.1007/s10068-013-0124-7
  17. Haminiuk CW, Plata-Oviedo MS, de Mattos G, Carpes ST, Branco IG. 2014. Extraction and quantification of phenolic acids and flavonols from Eugenia pyriformis using different solvents. J Food Sci Technol., 51:2862-2866 https://doi.org/10.1007/s13197-012-0759-z
  18. Hill MJ. 1998. Cereals, dietary fiber and cancer. Nutr Res., 18:653-659 https://doi.org/10.1016/S0271-5317(98)00051-7
  19. Horrobin DF, Huang YS. 1987. The role of linoleic acid and its metabolites in the lowering of plasma cholesterol and the prevention of cardiovascular disease. Int J Cardiol., 17:241-255 https://doi.org/10.1016/0167-5273(87)90073-8
  20. Huang Z, Wang B, Eaves DH, Shikany JM, Pace RD. 2007. Phenolic compound profile of selected vegetables frequently consumed by African Americans in the southeast United States. Food Chem., 103:1395-1402 https://doi.org/10.1016/j.foodchem.2006.10.077
  21. Kalogeropoulos N, Chiou A, Ioannou M, Karathanos VT, Hassapidou M, Andrikopoulos NK. 2010. Nutritional evaluation and bioactive microconstituents (phytosterols, tocopherols, polyphenols, triterpenic acids) in cooked dry legumes usually consumed in the Mediterranean countries. Food Chem., 121:628-690
  22. Kang CH, Park JK, Park JU, Chun SS, Lee SC, Ha JU, Hwang YI. 2002. Comparative studies on the fatty acid composition of Korean and Chinese sesame oils and adulterated sesame oils with commercial edible oils. J Korean Soc Food Sci Nutr., 31(1):17-20 https://doi.org/10.3746/jkfn.2002.31.1.017
  23. Kawaguchi K, Mizuno T, Aida K, Uchino K. 1997. Hesperidin as an inhibitor of lipases from porcine pancreas and Pseudomonas. Biosci Biotechnol Biochem., 61:102-104 https://doi.org/10.1271/bbb.61.102
  24. Kim MS, Park JH, Lim HJ, Kim DS, Kim HS, Lee KT, et al. 2017. Nutritional components and physicochemical properties of lipids extracted from forest resources. J Korean Soc FoodSci Nutr., 46:529-536 https://doi.org/10.3746/jkfn.2017.46.4.529
  25. Kim SY, Choi SW, Kim YS, Jeon SG, Seong KC. 2013. Production, marketing and domestic foreigners' consumption patterns of subtropical vegetables. KFMA., 30(3):29-54
  26. Oyaizu M. 1986. Studies on products of browning reaction:reaction; antioxidant activities of products of browning reaction prepared from glucoseamine. Jpn J Nutr Diet., 44:307-315 https://doi.org/10.5264/eiyogakuzashi.44.307
  27. Park Y, Sung J, Choi Y, Kim Y, Kim M, Jeong HS, Lee J. 2016. Analysis of vitamin E in agricultural processed foods in Korea. J Korean Soc Food Sci Nutr., 45:771-777 https://doi.org/10.3746/jkfn.2016.45.5.771
  28. Petropoulos S, Fernandes A, Barros L, Ferreira ICFR. 2018. Chemical composition, nutritional value and antioxidant properties of Mediterranean okra genotypes in relation to harvest stage. Food Chem., 242:466-474 https://doi.org/10.1016/j.foodchem.2017.09.082
  29. Radica MK, Viswanathan P, Anuradha CV. 2013. Nitric oxide mediates the insulin sensitizing effects of ${\beta}$-sitosterol in high fat diet-fed rats. Nitric Oxide., 32:43-53 https://doi.org/10.1016/j.niox.2013.04.007
  30. Scalbert A, Williamson G. 2000. Dietary intake and bioavailability of polyphenols. J Nutr., 130:2073S-2085S https://doi.org/10.1093/jn/130.8.2073S
  31. Shin EC, Pegg RB, Phillips RD, Eitenmiller RR. 2010. Commercial peanut (Arachis hypogaea L.) cultivars in the United States: phytosterol composition. J Agric Food Chem., 58:9137-9146 https://doi.org/10.1021/jf102150n
  32. Shin KA, Ko YS, Lee YC. 1998. Antioxidative effects and characteristics of methanol extracts from perilla oils roasted for different time. Korean J Food Sci Technol., 30:1045-1050
  33. Thanakosaia W, Phuwapraisirisan P. 2013. First identification of ${\alpha}$-glucosidase inhibitors from okra (Abelmoschus Esculentus) seeds. Nat Prod Commun., 8:1085-1088
  34. Uhiara NS, Onwuka G. 2014. Suitability of protein-rich extract from okra seed for formulation of ready to use therapeutic foods (RUTF). Niger Food J., 32:105-109 https://doi.org/10.1016/S0189-7241(15)30102-8
  35. Wie M, Seong J, Jeon K, Jung HS, Lee J. 2008. Comparison of vitamin E, phytosterols and fatty acid composition in commercially available grape seed oils in Korea. J Korean Soc Food Sci Nutr., 37(7):953-956 https://doi.org/10.3746/jkfn.2008.37.7.953
  36. Xuan TD, Gangqiang G, Minh TN, Quy TN, Khanh TD. 2018. An overview of chemical profiles, antioxidant and antimicrobial activities of commercial vegetable edible oils marketed in Japan. Foods., 7(2):21 https://doi.org/10.3390/foods7020021
  37. FAO. Food and Agriculture Organization Corporate Statistical Database. Available from:http://www.fao.org/faostat/en/#data/QC/visualize, [accessed 2020.07.30]