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Ecological Studies of Wild boars(Sus scrofa) in Yeongwol Hanbando Wetland Inferred through DNA Analysis of Non-invasive Samples

비침습 샘플 DNA 분석으로 유추한 영월 한반도습지 내 멧돼지(Sus scrofa)의 생태 연구

  • Kim, Minkyung (School of Biological Sciences, Seoul National University) ;
  • Lee, Sang-im (Department of New Biology, DGIST) ;
  • Park, Hyomin (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Lee, Sangdon (Department of Environmental Science and Engineering, Ewha Womans University)
  • 김민경 (서울대학교 생명과학부) ;
  • 이상임 (대구경북과학기술원 뉴바이올로지전공) ;
  • 박효민 (이화여자대학교 환경공학과) ;
  • 이상돈 (이화여자대학교 환경공학과)
  • Received : 2020.04.24
  • Accepted : 2020.05.27
  • Published : 2020.06.30

Abstract

This study inferred the ecology of habitat use of the wild boars (Sus scrofa) in Yeongwol Hanbando wetland through DNA analysis using non-invasive samples of hairs. From November 2018 to May 2019, hair samples were collected from rubbing trees and hairtraps within the Hanbando wetland (2.772 ㎢). We extracted DNA from the hair samples and conducted PCR to verify the species and identify sex of the individuals. In addition we analysed 6 microsatellite markers to identify individuals and genetic relationship among the pairs of individuals. A total of 16 boar hairs were sampled, which turned out to be from 10 individual (7 females and 3 males) boars. We found that 9 pairs, out of 45 possible pairs, were most likely to be relatives. The result from kinship data and the location of the sampled hairs suggest that wild boars in this area live as family groups that consist of a mother and her offspring, which is consistent with known habits of wild boars. It is needed to include more samples and microsatellite markers for better precise estimation of kinship among the boar individuals.

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