DOI QR코드

DOI QR Code

DNA Barcoding of Isaacsicalanus paucisetus (Copepoda: Calanoida: Spinocalanidae) from the Hydrothermal Vent in the North Fiji Basin, Southwestern Pacific Ocean

  • Park, Chailinn (Global Ocean Research Center, Korea Institute of Ocean Science and Technology) ;
  • Lee, Won-Kyung (Genome Editing Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Se-Joo (Genome Editing Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Ju, Se-Jong (Global Ocean Research Center, Korea Institute of Ocean Science and Technology)
  • 투고 : 2020.03.10
  • 심사 : 2020.04.14
  • 발행 : 2020.04.30

초록

Isaacsicalanus paucisetus Fleminger, 1983, a monotypic species of the family Spinocalanidae Vervoort, 1951, was first reported from a hydrothermal vent field in the East Pacific Rise off the mouth of the Gulf of California. The mitochondrial cytochrome oxidase I(mtCOI) DNA barcodes are considered a useful tool to assist traditional taxonomy and species discrimination in calanoid copepods. However, the mtCOI DNA barcodes of I. paucisetus have not been reported due to the species rarity and the difficulty of sampling. In this study, we firstly determined the mtCOI DNA barcodes of the I. paucisetus newly collected from a hydrothermal vent in the North Fiji Basin of the southwestern Pacific. All mtCOI DNA barcodes of I. paucisetus were identical and intraspecies variations of spinocalanid species were 0.0-3.0%. Interspecies and intergeneric variations were 13.4-25.2% and 16.7-24.1%, respectively. The DNA barcodes of I. paucisetus obtained in the present study would be helpful for understanding taxonomic relationships of widespread spinocalanid species.

과제정보

연구 과제번호 : Exploration for Seafloor Hydrothermal Deposits and Fe-Mn Crusts in the Southwestern Pacific and the Indian Ocean

연구 과제 주관 기관 : Korea Research Institute of Bioscience and Biotechnology (KRIBB)

참고문헌

  1. Baek SY, Jang KH, Choi EH, Ryu SH, Kim SK, Lee JH, Lim YJ, Lee J, Jun J, Kwak M, Lee YS, Hwang JS, Maran BAV, Chang CY, Kim IH, Hwang UW, 2016. DNA barcoding of metazoan zooplankton copepods from South Korea. PLoS One, 11:e0157307. https://doi.org/10.1371/journal.pone.0157307 https://doi.org/10.1371/journal.pone.0157307
  2. Blanco-Bercial L, Cornils A, Copley N, Bucklin A, 2014. DNA barcoding of marine copepods: assessment of analytical approaches to species identification. PLoS Currents Tree of Life, 6. https://doi.org/10.1371/currents.tol.cdf8b-74881f87e3b01d56b43791626d2
  3. Bode M, Laakmann S, Kaiser P, Hagen W, Auel H, Cornils A, 2017. Unravelling diversity of deep-sea copepods using integrated morphological and molecular techniques. Journal of Plankton Research, 39:600-617. https://doi.org/10.1093/plankt/fbx031 https://doi.org/10.1093/plankt/fbx031
  4. Boxshall GA, Halsey SH, 2004. An introduction to copepod diversity. The Ray Society, London, pp. 1-966.
  5. Bucklin A, Frost B, Bradford-Grieve J, Allen L, Copley N, 2003. Molecular systematic and phylogenetic assessment of 34 calanoid copepod species of the Calanidae and Clausocalanidae. Marine Biology, 142:333-343. https://doi.org/10.1007/s00227-002-0943-1 https://doi.org/10.1007/s00227-002-0943-1
  6. Bucklin A, Guarnieri M, Hill RS, Bentley AM, Kaartvedt S, 1999. Taxonomic and systematic assessment of planktonic copepods using mitochondrial COI sequence variation and competitive, species-specific PCR. Hydrobiologia, 401:239-254. https://doi.org/10.1007/978-94-011-4201-4_18 https://doi.org/10.1023/A:1003790411424
  7. Fleminger A, 1983. Description and phylogeny of Isaacsicalanus paucisetus, n. gen., n. sp., (Copepoda: Calanoida: Spinocalanidae) from an East Pacific hydrothermal vent site ($21^{\circ}N$). Proceedings of the Biological Society of Washington, 96:605-622.
  8. Ivanenko VN, Defaye D, Cuoc C, 2007. A new calanoid copepod (Spinocalanidae) swarming at a cold seep site on the Gabon continental margin (Southeast Atlantic). Cahiers de Biologie Marine, 48:37-54.
  9. Walter TC, Boxshall G, 2020. World of Copepods database. Spinocalanidae (Vervoort, 1951). Accessed 13 Apr 2020, .