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Administration of encapsulated L-tryptophan improves duodenal starch digestion and increases gastrointestinal hormones secretions in beef cattle

  • Lee, Sang-Bum (Department of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University) ;
  • Lee, Kyung-Won (Life Science Technology, Inc.) ;
  • Wang, Tao (Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Jilin Agricultural University) ;
  • Lee, Jae-Sung (Department of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University) ;
  • Jung, U-Suk (Department of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University) ;
  • Nejad, Jalil Ghassemi (Department of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University) ;
  • Oh, Young-Kyoon (Department of Nutrition and Physiology, National Institute of Animal Science, RDA) ;
  • Baek, Youl-Chang (Department of Nutrition and Physiology, National Institute of Animal Science, RDA) ;
  • Kim, Kyoung Hoon (Graduate School of International Agricultural Technology, Pyeongchang Campus, Seoul National University) ;
  • Lee, Hong-Gu (Department of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University)
  • Received : 2019.06.17
  • Accepted : 2019.10.30
  • Published : 2020.01.01

Abstract

Objective: This study investigated the effects of oral administration of rumen-protected L-tryptophan (RPL-T) on duodenal starch digestion and gastrointestinal hormones (GIH) secretions using Hanwoo beef steers as the animal models. Methods: Four steers (423±24 kg) fitted with ruminal and duodenal cannulas were employed in a crossover design replicated twice. Treatments were control (basal diet) and RPL-T (basal diet+191.1 mg/kg body weight [BW]) group. Blood and duodenal samples were collected to measure serum GIH levels and pancreatic α-amylase activity at day 0, 1, 3, and 5 (-30, 30, 90, 150, and 210 min) of the study. Samples from each segment of the gastrointestinal tract were collected via ruminal and duodenal cannulas and were used to determine soluble protein and the starch digestion rate at days 6 (-30, 180, 360, and 540 min) and 8 (-30, 90, 270, and 450 min) of the experiment. Results: No significant difference in ruminal pH, NH3-N, and total volatile fatty acid including the levels of acetate, propionate, butyrate, isobutyrate, valerate, isovalerate, and the acetate-to-propionate ratio was observed between groups (p>0.05). Crude protein uptake was higher and feces starch content was lower in RPL-T group than the control group (p<0.05). The D-glucose contents of feces in RPL-T group decreased at day 5 compared to those in the control group (p<0.05), however, no change was found at day 0, 1, or 3 compared to the control group (p>0.05). Serum cholecystokinin (CCK), melatonin, duodenal pancreatic α-amylase activity, and starch digestion were significantly higher in RPL-T group than the control group (p<0.05). Conclusion: Taken together, oral administration of RPL-T at the rate of 191.1 mg/kg BW consistently increased CCK concentration, pancreatic α-amylase activity in duodenal fluids, and starch digestion rate in the small intestine and thus found to be beneficial.

Acknowledgement

Supported by : Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry (IPET)

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