DOI QR코드

DOI QR Code

The effect of finishing diet supplemented with methionine/lysine and methionine/α-tocopherol on performance, carcass traits and meat quality of Hanwoo steers

  • Barido, Farouq Heidar (Department of Applied Animal Science, College of Animal Life Sciences, Kangwon National University) ;
  • Utama, Dicky Tri (Department of Applied Animal Science, College of Animal Life Sciences, Kangwon National University) ;
  • Jeong, Hae Seong (Department of Applied Animal Science, College of Animal Life Sciences, Kangwon National University) ;
  • Kim, Juntae (Department of Applied Animal Science, College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Chang Woo (Gangwon Province Livestock Research Institute) ;
  • Park, Yeon Soo (Gangwon Province Livestock Research Institute) ;
  • Lee, Sung Ki (Department of Applied Animal Science, College of Animal Life Sciences, Kangwon National University)
  • Received : 2019.03.14
  • Accepted : 2019.07.03
  • Published : 2020.01.01

Abstract

Objective: This study aimed to compare the effects of diets with and without supplements of methionine/lysine (met/lys) and methionine/α-tocopherol (met/α-tocopherol) on the performance, carcass traits and meat quality of Hanwoo steers. Methods: A total of 21 Hanwoo steers were divided into three different groups. Each group consisted of 7 animals that received different dietary treatments for 120 days as follows: a control (C) diet composed of a basal diet with 74% total digestible nutrient and 12% crude protein; treatment 1 (T1) composed of a basal diet enriched with methionine 69%+lysine 31%; and treatment (T2) composed of a basal diet enriched with methionine 84.65%+α-tocopherol 15.35%. Daily supplementation was given using the top-dressing method (20 g/animal). Results: The met/lys groups yielded a longissimus lumborum with increased water holding capacity (p<0.01) and lower shear force value (p<0.05). Dietary met/lys did not have an adverse effect on the animal performance and carcass traits. Instead, these supplements contributed significantly to the higher protein content compared to the control diet (p<0.05). Myristic acid (C14:0) was the only fatty acid affected by the supplementation. While the met/α-tocopherol group led to significantly higher protein and oxymyoglobin contents during storage (p<0.05). It also contributed significantly to the lower shear force value and 2-thiobarbituric acid reactive substances score during storage (p<0.05) compared to the control and treatment 1 since the initial storage day. The met/α-tocopherol diet also yielded meat with a redder color (p<0.05) after 3 days of storage. However, it did not significantly contribute to the fatty acid profiles of Hanwoo steers. Conclusion: Met/lys supplementation resulted in higher protein scores, water holding capacity and lower shear force scores. While met/α-tocopherol supplementation contributes to the production of redder meat, reduces lipid oxidation, production of more tender meat and increases the content of protein and oxymyoglobin percentage.

Acknowledgement

Supported by : Gangwon Provincial Livestock Research Institute

References

  1. Dantzer R, Mormede P. Stress in farm animals: a need for reevaluation. J Anim Sci 1983;57:6-18. https://doi.org/10.2527/jas1983.5716 https://doi.org/10.2527/jas1983.5716
  2. Wen C, Jiang XY, Ding LR, Wang T, Zhou YM. Effects of dietary methionine on growth performance, meat quality and oxidative status of breast muscle in fast- and slow-growing broilers. Poult Sci 2017;96:1707-14. https://doi.org/10. 3382/ps/pew432 https://doi.org/10.3382/ps/pew432
  3. Hosford AD, Hergenreder JE, Kim JK, et al. Effects of supplemental lysine and methionine with zilpaterol hydrochloride on feedlot performance, carcass merit, and skeletal muscle fiber characteristics in finishing feedlot cattle. J Anim Sci 2015;93:4532-44. https://doi.org/10.2527/jas.2015-9047 https://doi.org/10.2527/jas.2015-9047
  4. Zhai W, Schilling MW, Jackson V, Peebles ED, Mercier Y. Effect of dietary lysine and methionine supplementation on ross 708 male broilers from 21 to 42 days of age (II): breast meat quality. J Appl Poult Res 2016;25:212-22. https://doi.org/10.3382/japr/pfw003 https://doi.org/10.3382/japr/pfw003
  5. Baggerman JO. Role of encapsulated amino acids on skeletal muscle growth and development in feedlot steers [thesis]. Lubbock TX, USA: Texas Tech University; 2014.
  6. Min B, Nam KC, Cordray J, Ahn DU. Endogenous factors affecting oxidative stability of beef loin, pork loin, and chicken breast and thigh meats. J Food Sci 2008;73:C439-46. https://doi.org/10.1111/j.1750-3841.2008.00805.x https://doi.org/10.1111/j.1750-3841.2008.00805.x
  7. Liu Q, Lanari MC, Schaefer DM. A review of dietary vitamin e supplementation for improvement of beef quality. J Anim Sci 1995;73:3131-40. https://doi.org/10.2527/1995.73103131x https://doi.org/10.2527/1995.73103131x
  8. National Research Council. Nutrient requirements of beef cattle: seventh revised edition: update 2000. Washington, DC, USA: The National Academies Press; 2000.
  9. AOAC. Official Methods of Analysis. 17th edition. Gaithersburg, MD, USA: AOAC International; 2002.
  10. Kristensen L, Purslow PP. The effect of ageing on the water-holding capacity of pork: role of cytoskeletal proteins. Meat Sci 2001;58:17-23. https://doi.org/10.1016/S0309-1740(00) 00125-X https://doi.org/10.1016/S0309-1740(00)00125-X
  11. Utama DT, Ji HC, Chang WL, Park YS, Lee SK. Effect of mixed hay supplementation during fattening on carcass traits and meat quality of Hanwoo steers. J Anim Sci Technol 2017;59:6. https://doi.org/10.1186/s40781-017-0131-y https://doi.org/10.1186/s40781-017-0131-y
  12. Folch J, Lees M, Sloane SGH. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 1957;226:497-509.
  13. Park, BK, Choi NJ, Kim HC, et al. Effects of amino acid-enriched ruminally protected fatty acids on plasma metabolites, growth performance and carcass characteristics of Hanwoo steers. Asian-Australas J Anim Sci 2010;23:1013-21. https://doi.org/10.5713/ajas.2010.90559 https://doi.org/10.5713/ajas.2010.90559
  14. Prado IN, Campo MM, Muela E, et al. Effects of castration age, dietary protein level and lysine/methionine ratio on animal performance, carcass and meat quality of Friesian steers intensively reared. Animal 2014;8:1561-8. https://doi.org/10.1017/S1751731114001591 https://doi.org/10.1017/S1751731114001591
  15. Cano AB, Montano M, Salinas-Chavira J, Zinn RA. Evaluation of supplemental vitamin E on 56-day feedlot growth performance and plasma tocopherol concentrations in calf-fed Holstein steers. J Appl Anim Res 2017;45:90-2. https://doi.org/10.1080/09712119.2015.1129340 https://doi.org/10.1080/09712119.2015.1129340
  16. Brosnan, JT, Brosnan ME. The sulfur-containing amino acids: an overview. J Nutr 2006;136(Suppl):1636S-40S. https://doi.org/10.1093/jn/136.6.1636S https://doi.org/10.1093/jn/136.6.1636S
  17. Torrentera N, Carrasco R, Salinas-Chavira J, Plascencia A, Zinn RA. Influence of methionine supplementation of growing diets enriched with lysine on feedlot performance and characteristics of digestion in holstein steer calves. Asian-Australas J Anim Sci 2017;30:42-50. https://doi.org/10.5713/ajas.16.0181
  18. Lee SK, Panjono, Kangs SM, Kim TS, Park YS. The effects of dietary sulfur and vitamin E supplementation on the quality of beef from the longissimus muscle of Hanwoo bulls. Asian-Australas J Anim Sci 2008;21:1059-66. https://doi.org/10.5713/ajas.2008.70372 https://doi.org/10.5713/ajas.2008.70372
  19. Métayer S, Seiliez I, Collin A, et al. Mechanisms through which sulfur amino acids control protein metabolism and oxidative status. J Nutr Biochem 2008;19:207-15. https://doi.org/10.1016/j.jnutbio.2007.05.006 https://doi.org/10.1016/j.jnutbio.2007.05.006
  20. Giguere A, Girard CL, Matte JJ. Methionine, folic acid and vitamin B12 in growing-finishing pigs: impact on growth performance and meat quality. Arch Anim Nutr 2008;62:193-206. https://doi.org/10.1080/17450390802027494 https://doi.org/10.1080/17450390802027494
  21. Levine RL, Mosoni L, Berlett BS, Stadtman ER. Methionine residues as endogenous antioxidants in proteins. Proc Natl Acad Sci USA 1996;93:1536-40. https://doi.org/10.1073/pnas.93.26.15036
  22. Lee KW, Hwang YH, Joo ST. Meat tenderness characteristics of ten major muscles from Hanwoo steers according to quality grades of carcasses. Korean J Food Sci Anim Resour 2017;37:593-8. https://doi.org/10.5851/kosfa.2017.37.4.593 https://doi.org/10.5851/kosfa.2017.37.4.593
  23. Kellermeier JD, Tittor AW, Brooks JC, et al. Effects of zilpaterol hydrochloride with or without an estrogen-trenbolone acetate terminal implant on carcass traits, retail cutout, tenderness, and muscle fiber diameter in finishing steers. J Anim Sci 2009;87:3702-11. https://doi.org/10.2527/jas.2009-1823 https://doi.org/10.2527/jas.2009-1823
  24. Wegner J, Albrecht E, Fiedler I, et al. Growth- and breed-related changes of muscle fiber characteristics in cattle. J Anim Sci 2000;78:1485-96. https://doi.org/10.2527/2000.7861485x https://doi.org/10.2527/2000.7861485x
  25. Van Laack RLJM, Liu CH, Smith MO, Loveday HD. Characteristics of pale, soft, exudative broiler breast meat. Poult Sci 2000;79:1057-61. https://doi.org/10.1093/ps/79.7.1057 https://doi.org/10.1093/ps/79.7.1057
  26. Parr SL, Chung KY, Galyean ML, et al. Performance of finishing beef steers in response to anabolic implant and zilpaterol hydrochloride supplementation. J Anim Sci 2011;89:560-70. https://doi.org/10.2527/jas.2010-3101 https://doi.org/10.2527/jas.2010-3101
  27. Strasia, CA, Martin JJ, Gill DR, et al. Ruminal escape methionine and lysine for finishing steers. J Anim Sci 1986;63(Suppl 1):406.
  28. Schoonmaker JP, Cecava MJ, Fluharty FL, et al. Effect of source and amount of energy and rate of growth in the growing phase on performance and carcass characteristics of early- and normal-weaned beef steers. J Anim Sci 2004;82:273-82. https://doi.org/10.2527/2004.821273x https://doi.org/10.2527/2004.821273x
  29. Klemesrud MJ, Klopfenstein TJ, Stock RA, Lewis AJ, Herold DW. Effect of dietary concentration of metabolizable lysine on finishing cattle performance. J Anim Sci 2000;78:1060-6. https://doi.org/10.2527/2000.7841060x https://doi.org/10.2527/2000.7841060x