Illumina MiSeq sequencing reveals the effects of grape seed procyanidin on rumen archaeal communities in vitro

  • Zhang, Hua (Beijing Key Laboratory of Dairy Cow Nutrition, Beijing University of Agriculture) ;
  • Tong, Jinjin (Beijing Key Laboratory of Dairy Cow Nutrition, Beijing University of Agriculture) ;
  • Wang, Zun (Beijing Key Laboratory of Dairy Cow Nutrition, Beijing University of Agriculture) ;
  • Xiong, Benhai (State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences) ;
  • Jiang, Linshu (Beijing Key Laboratory of Dairy Cow Nutrition, Beijing University of Agriculture)
  • Received : 2019.03.19
  • Accepted : 2019.05.29
  • Published : 2020.01.01


Objective: The present study explored the effects of grape seed procyanidin extract (GSPE) on rumen fermentation, methane production and archaeal communities in vitro. Methods: A completely randomized experiment was conducted with in vitro incubation in a control group (CON, no GSPE addition; n = 9) and the treatment group (GSPE, 1 mg/bottle GSPE, 2 g/kg dry matter; n = 9). The methane and volatile fatty acid concentrations were determined using gas chromatography. To explore methane inhibition after fermentation and the response of the ruminal microbiota to GSPE, archaeal 16S rRNA genes were sequenced by MiSeq high-throughput sequencing. Results: The results showed that supplementation with GSPE could significantly inhibit gas production and methane production. In addition, GSPE treatment significantly increased the proportion of propionate, while the acetate/propionate ratio was significantly decreased. At the genus level, the relative abundance of Methanomassiliicoccus was significantly increased, while the relative abundance of Methanobrevibacter decreased significantly in the GSPE group. Conclusion: In conclusion, GSPE is a plant extract that can reduce methane production by affecting the structures of archaeal communities, which was achieved by a substitution of Methanobrevibacter with Methanomassiliicoccus.


Supported by : National Nature Science Foundation of China, Beijing Municipal Education Commission


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