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Effect of oral spray with Lactobacillus on growth performance, intestinal development and microflora population of ducklings

  • Zhang, Qi (Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University) ;
  • Jie, Yuchen (Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University) ;
  • Zhou, Chuli (Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University) ;
  • Wang, Leyun (Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University) ;
  • Huang, Liang (Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University) ;
  • Yang, Lin (Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University) ;
  • Zhu, Yongwen (Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University)
  • Received : 2019.01.15
  • Accepted : 2019.06.13
  • Published : 2020.03.01

Abstract

Objective: The aim of this study is to investigate the effect of oral spray with probiotics on the intestinal development and microflora colonization of hatched ducklings. Methods: In Exp. 1, an one-way factorial design was used to study the antibacterial activity of the probiotics and metabolites on Escherichia coli (E. coli) without antimicrobial resistance. There were four experimental groups including saline as control and Lactobacillus, Bacillus subtilis, combined Lactobacillus and Bacillus subtilis groups. In Exp. 2, 64-day-old ducklings were allotted to 2 treatments with 4 replicated pens. Birds in the control group were fed a basal diet supplemented with Lactobacillus fermentation in the feed whereas birds in the oral spray group were fed the basal diet and administrated Lactobacillus fermentation by oral spray way during the first week. Results: In Exp. 1, the antibacterial activities of probiotics and metabolites on E. coli were determined by the diameter of inhibition zone in order: Lactobacillus>combined Lactobacillus and Bacillus subtilis>Bacillus subtilis. Additionally, compared to E. coli without resistance, E. coli with resistance showed a smaller diameter of inhibition zones. In Exp. 2, compared to control feeding group, oral spray group increased (p<0.05) the final body weight at d 21 and average daily gain for d 1-21 and the absolute weight of the jejunum, ileum and total intestine tract as well as cecum Lactobacillus amount at d 21. Conclusion: Lactobacillus exhibited a lower antibacterial activity on E. coli with resistance than E. coli without resistance. Oral spray with Lactobacillus fermentation during the first week of could improve the intestinal development, morphological structure, and microbial balance to promote growth performance of ducklings from hatch to 21 d of age.

Acknowledgement

Supported by : National Natural Science Foundation of China, Guangdong Provincial Natural Science Foundation

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