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Effect of fermented blueberry on the oxidative stability and volatile molecule profiles of emulsion-type sausage during refrigerated storage

  • Zhou, Hengyue (Key Laboratory of Meat Processing and Quality Control, MOE, Key Laboratory of Meat Processing, MOA, Jiangsu Synergetic Innovation Center of Meat Processing and Quality Control, College of Food Science and Technology, Nanjing Agricultural University) ;
  • Zhuang, Xinbo (Key Laboratory of Meat Processing and Quality Control, MOE, Key Laboratory of Meat Processing, MOA, Jiangsu Synergetic Innovation Center of Meat Processing and Quality Control, College of Food Science and Technology, Nanjing Agricultural University) ;
  • Zhou, Changyu (Key Laboratory of Meat Processing and Quality Control, MOE, Key Laboratory of Meat Processing, MOA, Jiangsu Synergetic Innovation Center of Meat Processing and Quality Control, College of Food Science and Technology, Nanjing Agricultural University) ;
  • Ding, Daming (Key Laboratory of Meat Processing and Quality Control, MOE, Key Laboratory of Meat Processing, MOA, Jiangsu Synergetic Innovation Center of Meat Processing and Quality Control, College of Food Science and Technology, Nanjing Agricultural University) ;
  • Li, Chunbao (Key Laboratory of Meat Processing and Quality Control, MOE, Key Laboratory of Meat Processing, MOA, Jiangsu Synergetic Innovation Center of Meat Processing and Quality Control, College of Food Science and Technology, Nanjing Agricultural University) ;
  • Bai, Yun (Key Laboratory of Meat Processing and Quality Control, MOE, Key Laboratory of Meat Processing, MOA, Jiangsu Synergetic Innovation Center of Meat Processing and Quality Control, College of Food Science and Technology, Nanjing Agricultural University) ;
  • Zhou, Guanghong (Key Laboratory of Meat Processing and Quality Control, MOE, Key Laboratory of Meat Processing, MOA, Jiangsu Synergetic Innovation Center of Meat Processing and Quality Control, College of Food Science and Technology, Nanjing Agricultural University)
  • Received : 2019.02.01
  • Accepted : 2019.04.29
  • Published : 2020.05.01

Abstract

Objective: The aim of this work was to assess the effect of fermented blueberry (FB; 2%, 4%, and 6%) on the oxidative stability and volatile molecule profiles of emulsion-type sausage stored at 4℃ for 28 days. Methods: The antioxidant activity of FB was determined through radical-scavenging activity against 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radicals. Four formulations of sausage treatments with different FB levels (0%, 2%, 4%, 6%) were prepared, then peroxide value (POVs), thiobarbituric acid-reactive substances (TBARS) values, protein carbonyls and thiol groups were measured. The aroma profiles of sausages for each treatment was also determined. Results: The half maximal inhibitory concentration indicated that FB had greater scavenging ability than ascorbic acid against DPPH and hydroxyl radicals. Sausages with FB significantly retarded increases in POVs and TBARS, as well as in the content of protein carbonyls during all storage days (p<0.05). Particularly, 4% and 6% FB-treated sausages had better oxidation inhibition effects. However, FB accelerated the reduction in thiol groups (p<0.05). Additionally, FB inhibits the excessive formation of aldehyde compounds; for example, hexanal, which may cause rancid flavors, decreased from 58.25% to 19.41%. FB also created 6 alcohols (i.e., 2-methyl-1-propanol, 3-methyl-1-butanol, and phenylethyl alcohol), 5 ester compounds (i.e., ethyl acetate, ethyl lactate, and ethyl hexanoate) and 3-hydroxy-2-butanone in the sausages that contribute to sausage flavors. The principal component analysis showed that the aroma profiles of sausages with and without FB are easily identified. Conclusion: The addition of FB could significantly reduce the lipid and protein oxidation and improve oxidative stability for storage. Also, adding FB could inhibit rancid flavors and contribute to sausage flavors.

Acknowledgement

Supported by : Central Universities

References

  1. Feng X, Sebranek JG, Lee HY, Ahn DU. Effects of adding red wine on the physicochemical properties and sensory characteristics of uncured frankfurter-type sausage. Meat Sci 2016;121: 285-91. https://doi.org/10.1016/j.meatsci.2016.06.027 https://doi.org/10.1016/j.meatsci.2016.06.027
  2. Morrissey PA, Sheehy PJA, Galvin K, Kerry JP, Buckley DJ. Lipid stability in meat and meat products. Meat Sci 1998;49:S73-86. https://doi.org/10.1016/S0309-1740(98)90039-0 https://doi.org/10.1016/S0309-1740(98)90039-0
  3. Gray JI, Monahan FJ. Measurement of lipid oxidation in meat and meat products. Trends Food Sci Technol 1992;3:315-9. https://doi.org/10.1016/S0924-2244(10)80019-6 https://doi.org/10.1016/S0924-2244(10)80019-6
  4. Estevez M. Protein carbonyls in meat systems: A review. Meat Sci 2011;89:259-79. https://doi.org/10.1016/j.meatsci.2011.04.025 https://doi.org/10.1016/j.meatsci.2011.04.025
  5. Ozvural EB, Vural H. Grape seed flour is a viable ingredient to improve the nutritional profile and reduce lipid oxidation of frankfurters. Meat Sci 2011;88:179-83. https://doi.org/10.1016/j.meatsci.2010.12.022 https://doi.org/10.1016/j.meatsci.2010.12.022
  6. Botterweck AA, Verhagen H, Goldbohm RA, Kleinjans J, Van Den Brandt PA. Intake of butylated hydroxyanisole and butylated hydroxytoluene and stomach cancer risk: results from analyses in the Netherlands Cohort Study. Food Chem Toxicol 2000;38:599-605. https://doi.org/10.1016/S0278-6915(00)00042-9 https://doi.org/10.1016/S0278-6915(00)00042-9
  7. Muzolf-Panek M, Waskiewicz A, Kowalski R, Konieczny P. The effect of blueberries on the oxidative stability of pork meatloaf during chilled storage. J Food Process Preserv 2016;40:899-909. https://doi.org/10.1111/jfpp.12668 https://doi.org/10.1111/jfpp.12668
  8. Coloretti F, Tabanelli G, Chiavari C, et al. Effect of wine addition on microbiological characteristics, volatile molecule profiles and biogenic amine contents in fermented sausages. Meat Sci 2014;96:1395-402. https://doi.org/10.1016/j.meatsci.2013.11.027 https://doi.org/10.1016/j.meatsci.2013.11.027
  9. Gardini F, Tabanelli G, Lanciotti R, et al. Biogenic amine content and aromatic profile of Salama da sugo, a typical cooked fermented sausage produced in Emilia Romagna region (Italy). Food Control 2013;32:638-43. https://doi.org/10.1016/j.food cont.2013.01.039 https://doi.org/10.1016/j.foodcont.2013.01.039
  10. Castrejon ADR, Eichholz I, Rohn S, Kroh LW, Huyskens-Keil S. Phenolic profile and antioxidant activity of highbush blueberry (Vaccinium corymbosum L.) during fruit maturation and ripening. Food Chem 2008;109:564-72. https://doi.org/10.1016 /j.foodchem.2008.01.007 https://doi.org/10.1016/j.foodchem.2008.01.007
  11. Su MS, Chien PJ. Antioxidant activity, anthocyanins, and phenolics of rabbiteye blueberry (Vaccinium ashei) fluid products as affected by fermentation. Food Chem 2007;104:182-7. https://doi.org/10.1016/j.foodchem.2006.11.021 https://doi.org/10.1016/j.foodchem.2006.11.021
  12. Oh BT, Jeong SY, Velmurugan P, Park JH, Jeong DY. Probiotic-mediated blueberry (Vaccinium corymbosum L.) fruit fermentation to yield functionalized products for augmented antibacterial and antioxidant activity. J Biosci Bioeng 2017;124:542-50. https://doi.org/10.1016/j.jbiosc.2017.05.011 https://doi.org/10.1016/j.jbiosc.2017.05.011
  13. Jiang ZL. Study on mechanism, metabolic process and bioactivities of microbial natural-ferments during fermation [PhD Thesis]. Hangzhou, China: Zhejiang Gongshang University; 2017.
  14. Su MS, Chien PJ. Aroma impact components of rabbiteye blueberry (Vaccinium ashei) vinegars. Food Chem 2010;119:923-8. https://doi.org/10.1016/j.foodchem.2009.07.053 https://doi.org/10.1016/j.foodchem.2009.07.053
  15. Jia N, Kong B, Liu Q, Diao X, Xia X. Antioxidant activity of black currant (Ribes nigrum L.) extract and its inhibitory effect on lipid and protein oxidation of pork patties during chilled storage. Meat Sci 2012;91:533-9. https://doi.org/10.1016/j.meatsci.2012.03.010 https://doi.org/10.1016/j.meatsci.2012.03.010
  16. Xu P, Zheng Y, Zhu X, Li S, Zhou C. L-lysine and L-arginine inhibit the oxidation of lipids and proteins of emulsion sausage by chelating iron ion and scavenging radical. Asian-Australas J Anim Sci 2018;31:905-13. https://doi.org/10.5713/ajas.17.0617 https://doi.org/10.5713/ajas.17.0617
  17. Pil-Nam S, Park KM, Kang GH, Cho SH, Park BY, Van-Ba H. The impact of addition of shiitake on quality characteristics of frankfurter during refrigerated storage. LWT - Food Sci Technol 2015;62:62-8. https://doi.org/10.1016/j.lwt.2015.01.032 https://doi.org/10.1016/j.lwt.2015.01.032
  18. Zhang L, Lin YH, Leng XJ, Huang M, Zhou GH. Effect of sage (Salvia officinalis) on the oxidative stability of Chinese-style sausage during refrigerated storage. Meat Sci 2013;95:145-50. https://doi.org/10.1016/j.meatsci.2013.05.005 https://doi.org/10.1016/j.meatsci.2013.05.005
  19. Jongberg S, Skov SH, Torngren MA, Skibsted LH, Lund MN. Effect of white grape extract and modified atmosphere packaging on lipid and protein oxidation in chill stored beef patties. Food Chem 2011;128:276-83. https://doi.org/10.1016/j.food chem.2011.03.015 https://doi.org/10.1016/j.foodchem.2011.03.015
  20. Benet I, Guardia MD, Ibanez C, Sola J, Arnau J, Roura E. Analysis of SPME or SBSE extracted volatile compounds from cooked cured pork ham differing in intramuscular fat profiles. LWT - Food Sci Technol 2015;60:393-9. https://doi.org/10.1016/j.lwt.2014.08.016 https://doi.org/10.1016/j.lwt.2014.08.016
  21. Min-Sheng S, Po-Jung C. Antioxidant activity, anthocyanins, and phenolics of rabbiteye blueberry (Vaccinium ashei) fluid products as affected by fermentation. Food Chem 2007;104:182-7. https://doi.org/10.1016/j.foodchem.2006.11.021 https://doi.org/10.1016/j.foodchem.2006.11.021
  22. Sherwin ER. Oxidation and antioxidants in fat and oil processing. J Am Oil Chem Soc 1978;55:809-14. https://doi.org/10.1007/BF02682653 https://doi.org/10.1007/BF02682653
  23. Ruiz A, CanAda MJ, Ayora, Lendl B. A rapid method for peroxide value determination in edible oils based on flow analysis with Fourier transform infrared spectroscopic detection. Analyst 2001;126:242-6. https://doi.org/10.1039/B008688F https://doi.org/10.1039/b008688f
  24. Dobarganes MC, Velasco J. Analysis of lipid hydroperoxides. Eur J Lipid Sci Technol 2002;104:420-8. https://doi.org/10.1002/1438-9312(200207)104:7 https://doi.org/10.1002/1438-9312(200207)104:7<420::AID-EJLT420>3.0.CO;2-N
  25. Ulu H. Evaluation of three 2-thiobarbituric acid methods for the measurement of lipid oxidation in various meats and meat products. Meat Sci 2004;67:683-7. https://doi.org/10.1016/j.meatsci.2003.12.014 https://doi.org/10.1016/j.meatsci.2003.12.014
  26. Skrovankova S, Sumczynski D, Mlcek J, Jurikova T, Sochor J. Bioactive compounds and antioxidant activity in different types of berries. Int J Mol Sci 2015;16:24673-706. https://doi.org/10.3390/ijms161024673 https://doi.org/10.3390/ijms161024673
  27. Kumar Y, Ahmad T, Narsaiah K. Recent trends in the use of natural antioxidants for meat and meat products. Compr Rev Food Sci Food Saf 2015;14:796-812. https://doi.org/10.1111/1541-4337.12156 https://doi.org/10.1111/1541-4337.12156
  28. Jongberg S, Torngren MA, Gunvig A, Skibsted LH, Lund MN. Effect of green tea or rosemary extract on protein oxidation in Bologna type sausages prepared from oxidatively stressed pork. Meat Sci 2013;93:538-46. https://doi.org/10.1016/j.meatsci.2012.11.005 https://doi.org/10.1016/j.meatsci.2012.11.005
  29. Amici A, Levine RL, Tsai L, Stadtman E. Conversion of amino acid residues in proteins and amino acid homopolymers to carbonyl derivatives by metal-catalyzed oxidation reactions. J Bio Chem 1989;264:3341-6.
  30. Berlett BS, Stadtman ER. Protein oxidation in aging, disease, and oxidative stress. J Biol Chem 1997;272:20313-6. https://doi.org/10.1074/jbc.272.33.20313 https://doi.org/10.1074/jbc.272.33.20313
  31. Burcham PC, Kuhan YT. Introduction of carbonyl groups into proteins by the lipid peroxidation product, malondialdehyde. Biochem Biophys Res Commun 1996;220:996-1001. https://doi.org/10.1006/bbrc.1996.0521 https://doi.org/10.1006/bbrc.1996.0521
  32. Sante-Lhoutellier V, Engel E, Aubry L, Gatellier P. Effect of animal (lamb) diet and meat storage on myofibrillar protein oxidation and in vitro digestibility. Meat Sci 2008;79:777-83. https://doi.org/10.1016/j.meatsci.2007.11.011 https://doi.org/10.1016/j.meatsci.2007.11.011
  33. Estevez M, Ventanas S, Cava R. Effect of natural and synthetic antioxidants on protein oxidation and colour and texture changes in refrigerated stored porcine liver pate. Meat Sci 2006;74:396-403. https://doi.org/10.1016/j.meatsci.2006.04.010 https://doi.org/10.1016/j.meatsci.2006.04.010
  34. Estevez M, Heinonen M. Effect of phenolic compounds on the formation of alpha-aminoadipic and gamma-glutamic semialdehydes from myofibrillar proteins oxidized by copper, iron, and myoglobin. J Agric Food Chem 2010;58:4448-55. https://doi.org/10.1021/jf903757h https://doi.org/10.1021/jf903757h
  35. Estevez M, Kylli P, Puolanne E, Kivikari R, Heinonen M. Oxidation of skeletal muscle myofibrillar proteins in oil-in-water emulsions: interaction with lipids and effect of selected phenolic compounds. J Agric Food Chem 2008;56:10933-40. https://doi.org/10.1021/jf801784h https://doi.org/10.1021/jf801784h
  36. Lara MS, Gutierrez JI, Timon M, Andres AI. Evaluation of two natural extracts (Rosmarinus officinalis L. and Melissa officinalis L.) as antioxidants in cooked pork patties packed in MAP. Meat Sci 2011;88:481-8. https://doi.org/10.1016/j.meatsci.2011.01.030 https://doi.org/10.1016/j.meatsci.2011.01.030
  37. Hur SJ, Kim DH, Chun SC, Lee SK. Antioxidative Changes of blueberry leaf extracts in emulsion-type sausage during in vitro digestion. Korean J Food Sci Anim Resour 2013;33:689-95. https://doi.org/10.5851/kosfa.2013.33.6.689 https://doi.org/10.5851/kosfa.2013.33.6.689
  38. Xiang C, Ruiz-Carrascal J, Petersen MA, Karlsson AH. Cheese powder as an ingredient in emulsion sausages: Effect on sensory properties and volatile compounds. Meat Sci 2017;130:1-6. https://doi.org/10.1016/j.meatsci.2017.03.009 https://doi.org/10.1016/j.meatsci.2017.03.009
  39. Ajuyah AO, Fenton TW, Hardin RT, Sim JS. Measuring lipid oxidation volatiles in meats. J Food Sci 2010;58:270-3. https://doi.org/10.1111/j.1365-2621.1993.tb04253.x
  40. Drumm TD, Spanier AM. Changes in the content of lipid autoxidation and sulfur-containing compounds in cooked beef during storage. J Agric Food Chem 1991;39:336-43. https://doi.org/10.1021/jf00002a023 https://doi.org/10.1021/jf00002a023
  41. Stahnke LH. Volatiles produced by Staphylococcus xylosus and Staphylococcus carnosus during growth in sausage minces part II. The influence of growth parameters. LWT-Food Sci Technol 1999;32:365-71. https://doi.org/10.1006/fstl.1999.0560 https://doi.org/10.1006/fstl.1999.0560
  42. Dupuy HP, Bailey ME, Angelo AJS, Vercellotti JR, Legendre MG. Instrumental analyses of volatiles related to warmed-over flavor of cooked meats. Warmed-over Flavor of Meat; 1987. pp. 165-91.
  43. Delgado FJ, Gonzalez-Crespo J, Cava R, Garcia-Parra J, Ramirez R. Characterisation by SPME-GC-MS of the volatile profile of a Spanish soft cheese P.D.O. Torta del Casar during ripening. Food Chem 2010;118:182-9. https://doi.org/10.1016/j.food chem.2009.04.081 https://doi.org/10.1016/j.foodchem.2009.04.081
  44. Olivares A, Navarro JL, Flores M. Establishment of the contribution of volatile compounds to the aroma of fermented sausages at different stages of processing and storage. Food Chem 2009;115:1464-72. https://doi.org/10.1016/j.foodchem.2009.01.083 https://doi.org/10.1016/j.foodchem.2009.01.083
  45. Coloretti F, Tabanelli G, Chiavari C, et al. Effect of wine addition on microbiological characteristics, volatile molecule profiles and biogenic amine contents in fermented sausages. Meat Sci 2014;96:1395-402. https://doi.org/10.1016/j.meatsci.2013.11.027 https://doi.org/10.1016/j.meatsci.2013.11.027
  46. Mateo J, Zumalacarregui J. Volatile compounds in chorizo and their changes during ripening. Meat Sci 1996;44:255-73. https://doi.org/10.1016/S0309-1740(96)00028-9 https://doi.org/10.1016/S0309-1740(96)00028-9
  47. Verbeek CJR, Hicks T, Langdon A. Odorous compounds in bioplastics derived from bloodmeal. J Am Oil Chem Soc 2012;89:529-40. https://doi.org/10.1007/s11746-011-1939-0 https://doi.org/10.1007/s11746-011-1939-0