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Effect of addition of methanol on rheological properties of silk formic acid solution

  • Bae, Yu Jeong (Department of Biofibers and Biomaterials Science, Kyungpook National University) ;
  • Um, In Chul (Department of Biofibers and Biomaterials Science, Kyungpook National University)
  • Received : 2020.02.12
  • Accepted : 2020.03.10
  • Published : 2020.03.31

Abstract

Recently, many studies have been undertaken on the wet spinning and electrospinning of silk because wet-spun fibers and electrospun webs of silk can be applied in the biomedical and cosmetic fields owing to the good biocompatibility of silk. The rheological properties of silk solution are important because they strongly affect the spinning performance of the silk solution and the structures of resultant fibrous materials. Therefore, as a preliminary study on the effect of solvent composition on the rheological properties of silk fibroin (SF) solution and structure of the resultant film, in the reported work, methanol was added to the SF formic acid solution. A small amount of methanol (i.e. 2%) added to the SF formic acid solution significantly altered the rheological properties of the solution: its shear viscosity increased by 10 folds at low shear and decreased on increasing the shear rate, demonstrating shear thinning behavior of the SF solution. Dynamic tests for the SF solution indicated that the addition of 2% methanol altered the viscous state of the SF formic acid solution to elastic. However, the molecular conformation (i.e. β-sheet conformation) of the regenerated SF film cast from formic acid remained unchanged on the addition of 2% methanol.

Acknowledgement

Supported by : National Research Foundation of Korea (NRF)

References

  1. Cho HJ, Ki CS, Oh J, Lee KH, Um IC (2012) Molecular weight distribution and solution properties of silk fibroins with different dissolution conditions. Int J Biol Macromol 51, 336-341. https://doi.org/10.1016/j.ijbiomac.2012.06.007
  2. Cho HJ, Yoo YJ, Kim JW, Park YH, Bae DG, Um IC (2012) Effect of molecular weight and storage time on the wet- and electro-spinning of regenerated silk fibroin. Polym Degrad Stabil 97, 1060-1066. https://doi.org/10.1016/j.polymdegradstab.2012.03.007
  3. Chung DE, Kim HH, Kim MK, Lee KH, Park YH, Um IC (2015) Effects of different Bombyx mori silkworm varieties on the structural characteristics and properties of silk. Int J Biol Macromol 79, 943-951. https://doi.org/10.1016/j.ijbiomac.2015.06.012
  4. Chung DE, Um IC (2014) Effect of molecular weight and concentration on crystallinity and post drawing of wet spun silk fibroin fiber. Fiber Polym 15, 153-160. https://doi.org/10.1007/s12221-014-0153-8
  5. Ha SW, Tonelli AE, Hudson SM (2005) Structural studies of Bombyx mori silk fibroin during regeneration from solutions and wet fiber spinning. Biomacromolecules 6, 1722-1731. https://doi.org/10.1021/bm050010y
  6. Jang MJ, Um IC (2015) Effect of different Bombyx mori silkworm varieties on the wet spinning of silk fibroin. Int J Indust Entomol 30, 75-80. https://doi.org/10.7852/ijie.2015.30.2.75
  7. Jo YN, Um IC (2015) Effects of solvent on the solution properties, structural characteristics and properties of silk sericin. Int J Biol Macromol 78, 287-295. https://doi.org/10.1016/j.ijbiomac.2015.04.004
  8. Ki CS, Kim JW, Oh HJ, Lee KH, Park YH (2007) The effect of residual silk sericin on the structure and mechanical property of regenerated silk filament. Int J Biol Macromol 41, 346-353. https://doi.org/10.1016/j.ijbiomac.2007.05.005
  9. Kim HJ, Um IC (2014) Relationship between the rheology and electro-spinning performance of regenerated silk fibroin prepared using different degumming methods. Korea-Aust Rheol J 26, 119-125. https://doi.org/10.1007/s13367-014-0012-6
  10. Ko JS, Ki CS, Um IC (2018) Effect of sericin content on the structural characteristics and properties of electro-spun regenerated silk. Fiber Polym 19, 507-514. https://doi.org/10.1007/s12221-018-7861-4
  11. Meinel L, Hofmann S, Karageorgiou V, Kirker-Head C, McCool J, Gronowicz G, et al. (2005) The inflammatory responses to silk films in vitro and in vivo. Biomaterials 26, 147-155. https://doi.org/10.1016/j.biomaterials.2004.02.047
  12. Minoura N, Aiba S, Gotoh Y, Tsukada M, Imai Y (1995) Attachment and growth of cultured fibroblast cells on silk protein matrices. J Biomed Mater Res 29, 1215-1221. https://doi.org/10.1002/jbm.820291008
  13. Park BK, Nho SK, Um IC (2019a) Molecular conformation and crystallinity of white colored silkworm cocoons with different silkworm varieties. Int J Indust Entomol 38, 18-23.
  14. Park BK, Nho SK, Um IC (2019b) Crystallinity of yellow colored silkworm variety cocoons. Int J Indust Entomol 38, 51-55.
  15. Park BK, Um IC (2015) Effect of Korean Bombyx mori variety on electro-spinning performance of regenerated silk fibroin. Fiber Polym 16, 1935-1940. https://doi.org/10.1007/s12221-015-5472-x
  16. Park BK, Um IC (2017) Effects of electric field on the maximum electro-spinning rate of silk fibroin solutions. Int J Biol Macromol 95, 8-13. https://doi.org/10.1016/j.ijbiomac.2016.11.026
  17. Park BK, Um IC (2018) Effect of molecular weight on electro-spinning performance of regenerated silk. Int J Biol Macromol 106, 1166-1172. https://doi.org/10.1016/j.ijbiomac.2017.08.115
  18. Park CJ, Um IC (2018) Effect of heat treatment on the structural characteristics and properties of silk sericin film. Int J Indust Entomol 37, 36-42.
  19. Sakabe H, Ito H, Miyamoto T, Noishiki Y, Ha WS (1989) In vivo blood compatibility of regenerated silk fibroin. Sen'i Gakkaishi 45, 487-490. https://doi.org/10.2115/fiber.45.11_487
  20. Um IC, Kweon H, Lee KG, Ihm DW, Lee J, Park YH (2004) Wet spinning of silk polymer: I. Effect of coagulation conditions on the morphological feature of filament. Int J Biol Macromol 34, 89-105. https://doi.org/10.1016/j.ijbiomac.2004.03.007
  21. Um IC, Kweon HY, Hwang CM, Min BG, Park YH (2002) Structural characteristics and properties of silk fibroin/polyurethane blend films. Int J Indust Entomol 5, 163-170.
  22. Um IC, Kweon HY, Park YH, Hudson S (2001) Structural characteristics and properties of the regenerated silk fibroin prepared from formic acid. Int J Biol Macromol 29, 91-97. https://doi.org/10.1016/S0141-8130(01)00159-3
  23. Yoon K, Lee HN, Ki CS, Fang D, Hsiao BS, Chu B, et al. (2013) Effect of degumming conditions on electro-spinning rate of regenerated silk. Int J Biol Macromol 61, 50-57. https://doi.org/10.1016/j.ijbiomac.2013.06.039