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Functional analysis of the antioxidant activity of immune-challenged Bombyx mori hemolymph extracts in the human epithelial Caco-2 cell line

  • Kim, Seong Ryul (Sericultural and Apicultural Materials Division, National Institute of Agricultural Sciences, RDA) ;
  • Kim, Kee-Young (Sericultural and Apicultural Materials Division, National Institute of Agricultural Sciences, RDA) ;
  • Kim, Seong-Wan (Sericultural and Apicultural Materials Division, National Institute of Agricultural Sciences, RDA) ;
  • Park, Seung-Won (Department of Biomedical Science, Daegu Catholic University)
  • Received : 2020.02.24
  • Accepted : 2020.03.13
  • Published : 2020.03.31

Abstract

Humans use insects as food and traditional medicine for many years. Hemolymph is the circulating fluid of insects and is a key component of their immune system. However, limited information is available regarding hemolymph identification, development, and differentiation, as well as the related cellular immune responses. In a previous study, hemolymph extracts prepared from Bombyx mori larvae were found to exert anti-inflammatory effects. In this study, we aimed to identify and compare the antioxidant activity of immune-challenged and unchallenged B. mori hemolymph extracts in vitro. For this purpose, human epithelial Caco-2 cells were first exposed to oxidative stress and then treated with various concentrations and incubation times of either immune-challenged or unchallenged B. mori hemolymph extracts. Next, we determined the effect of treatment on the relative expression of GPX-1, SOD-1, and SOD-2 antioxidant marker genes. We found that the expression rates of the three marker genes were markedly higher at a immune-challenged hemolymph extract concentration of 80 ppm compared to those at other concentrations, and the antioxidant effects were enhanced after treatment for 48 hr. Thus, B. mori hemolymph extracts showed antioxidant activity within the limited time and dose. Especially, the immune-challenged B. mori hemolymph extracts showed higher the antioxidant activities than unchallenged one. The activity of silkworm hemolymph extracts could facilitate the development of new types of functional foods, feed additives, and biomaterials with antioxidant properties.

Acknowledgement

Supported by : Rural Development Administration

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