DOI QR코드

DOI QR Code

Morphology, molecular conformation and moisture regain of cocoons of different silkworm varieties

  • Choi, Hui Ju (Department of Biofibers and Biomaterials Science, Kyungpook National University) ;
  • Noh, Si Kab (Department of Biofibers and Biomaterials Science, Kyungpook National University) ;
  • Um, In Chul (Department of Biofibers and Biomaterials Science, Kyungpook National University)
  • Received : 2020.02.11
  • Accepted : 2020.03.09
  • Published : 2020.03.31

Abstract

Silk has attracted the attention of numerous research groups because of its unique properties as a biomedical material. Recently, studies on the biomedical application of silk cocoon, such as membranes for guided bone regeneration, have been conducted. As a preliminary study on silkworm cocoons, the morphological structure and crystallinity of cocoon of nine silkworm varieties with different sides are examined in this study. The morphological structure of the silkworm cocoon is shown to be dependent on silkworm variety and the cocoon layer (i.e. outside or inside). It is also shown that the silk filaments of the out layer are more bent and twisted compared to those of the inner layer. Furthermore, the crystallinity of the outer surface of all silkworm cocoons is found to be higher than that of the inner surface, regardless of the silkworm variety. Lastly, it is shown that the crystallinity index of the cocoon depends on the silkworm variety, implying that the structure of the cocoon varies with silkworm variety.

Acknowledgement

Supported by : National Research Foundation of Korea (NRF)

References

  1. Chung DE, Kim HH, Kim MK, Lee KH, Park YH, Um IC (2015a) Effects of different Bombyx mori silkworm varieties on the structural characteristics and properties of silk. Int J Biol Macromol 79, 943-951 https://doi.org/10.1016/j.ijbiomac.2015.06.012
  2. Chung DE, Lee JH, Kweon HY, Lee KG, Um IC (2015b) Structure and properties of silk sericin obtained from different silkworm varieties. Int J Indust Entomol, 30, 81-85. https://doi.org/10.7852/ijie.2015.30.2.81
  3. Chung DE, Um IC (2014) Effect of molecular weight and concentration on crystallinity and post drawing of wet spun silk fibroin fiber. Fiber Polym 15, 153-160. https://doi.org/10.1007/s12221-014-0153-8
  4. Kim HJ, Um IC (2014) Effect of degumming ratio on wet spinning and post drawing performance of regenerated silk. Int J Biol Macromol 67, 387-393. https://doi.org/10.1016/j.ijbiomac.2014.03.055
  5. Kim J, Kim CH, Park CH, Seo JN, Kweon HY, Kang SW et al. (2010) Comparison of methods for the repair of acute tympanic membrane perforations: silk patch vs. paper patch. Wound Rep Reg 18, 132-138. https://doi.org/10.1111/j.1524-475X.2009.00565.x
  6. Kim SG, Kim MK, Kweon H, Jo YY, Lee KG, Lee JK (2016) Comparison of unprocessed silk cocoon and silk cocoon middle layer membranes for guided bone regeneration. Maxillofac Plast Reconstr Surg, 38, 11. https://doi.org/10.1186/s40902-016-0057-1
  7. Kweon HY, Lee KG, Chae CH, Balazsi C, Min SK, Kim JY et al. (2011) Development of nano-hydroxyapatite graft with silk fibroin scaffold as a new bone substitute. J Oral Maxillofac Surg 69, 1578-1586. https://doi.org/10.1016/j.joms.2010.07.062
  8. Kweon HY, Lee KG, Park KY, Kang SW, Kang PD, Kim MJ et al. (2012b) Cocoon filament quality of a special silkworm variety, GoldenSilk. Int J Indust Entomol 24, 17-21. https://doi.org/10.7852/ijie.2012.24.1.017
  9. Kweon HY, Lee KG, Park KY, Kang SW, Seok YS (2012a) Degumming characteristics and color stability of GoldenSilk cocoon. Int J Indust Entomol 24, 1-5. https://doi.org/10.7852/ijie.2012.24.1.001
  10. Lee WY, Um IC, Kim MK, Kwon KJ, Kim SG, Park YW (2014) Effectiveness of woven Silk dressing materials on full-skin thickness burn wounds in rat model. Maxillofac Plast Reconstr Surg 36, 280-284. https://doi.org/10.14402/jkamprs.2014.36.6.280
  11. Minoura N, Aiba S, Gotoh Y, Tsukada M, Imai Y (1995) Attachment and growth of cultured fibroblast cells on silk protein matrices. J Biomed Mater Res 29, 1215-1221. https://doi.org/10.1002/jbm.820291008
  12. Park BK, Um IC (2015) Effect of Korean Bombyx mori variety on electro-spinning performance of regenerated silk fibroin. Fiber Polym 16, 1935-1940. https://doi.org/10.1007/s12221-015-5472-x
  13. Park BK, Um IC (2018) Effect of molecular weight on electro-spinning performance of regenerated silk. Int J Biol Macromol 106, 1166-1172. https://doi.org/10.1016/j.ijbiomac.2017.08.115
  14. Park BK, Nho SK, Um IC (2019a) Molecular conformation and crystallinity of white colored silkworm cocoons with different silkworm varieties. Int J Indust Entomol 38, 18-23.
  15. Park BK, Nho SK, Um IC (2019b) Crystallinity of yellow colored silkworm variety cocoons. Int J Indust Entomol 38, 51-55.
  16. Park CJ, Ryoo JY, Ki CS, Kim JW, Kim IS, Bae DG et al. (2018) Effect of molecular weight on the structure and mechanical properties of silk sericin gel, film and sponge. Int J Biol Macromol 119, 821-832. https://doi.org/10.1016/j.ijbiomac.2018.08.006
  17. Sakabe H, Ito H, Miyamoto T, Noishiki Y, Ha WS (1989) In vivo blood compatibility of regenerated silk fibroin. Sen'i Gakkaishi 45, 487-490. https://doi.org/10.2115/fiber.45.11_487
  18. Seok H, Kim MK, Kim SG, Kweon H (2014) Comparison of silkworm-cocoon-derived silk membranes of two different thicknesses for guided bone regeneration. J Craniofac Surg 25, 2066-2069. https://doi.org/10.1097/SCS.0000000000001151
  19. Song JY, Kim SG, Lee JW, Chae WS, Kweon HY, Jo YY et al. (2011) Accelerated healing with the use of a silk fibroin membrane for the guided bone regeneration technique. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 112, e26-e33.
  20. Um IC, Kweon HY, Hwang CM, Min BG, Park YH (2002) Structural characteristics and properties of silk fibroin/polyurethane blend films. Int J Indust Entomol 5, 163-170.
  21. Um IC, Kweon HY, Park YH, Hudson S (2001) Structural characteristics and properties of the regenerated silk fibroin prepared from formic acid. Int J Biol Macromol 29, 91-97. https://doi.org/10.1016/S0141-8130(01)00159-3