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Di(2-ethylhexyl) Phthalate Induces the Apoptotic Cell Death Mediated by Production of Reactive Oxygen Species in Human Keratinocyte

미세먼지의 di(2-ethylhexyl) phthalate가 유도한 피부상피세포 사멸 신호전달기전 연구

  • Park, Jeong-Bae (Department of Pharmaceutical Engineering, Daegu Haany University) ;
  • Kim, Ji-Yun (Department of Pharmaceutical Engineering, Daegu Haany University) ;
  • Sung, Junghee (R&D center, Reanzen Co.,Ltd.) ;
  • Kim, Yong-ung (Department of Pharmaceutical Engineering, Daegu Haany University) ;
  • Lee, Sei-Jung (Department of Pharmaceutical Engineering, Daegu Haany University)
  • 박정배 (대구한의대학교 제약공학과) ;
  • 김지윤 (대구한의대학교 제약공학과) ;
  • 성정희 (리엔젠 부설연구소) ;
  • 김용웅 (대구한의대학교 제약공학과) ;
  • 이세중 (대구한의대학교 제약공학과)
  • Received : 2019.12.13
  • Accepted : 2020.02.12
  • Published : 2020.03.31

Abstract

Particulate matter with an aerodynamic diameter of less than 2.5 μM (PM2.5) is one of the major environmental pollutants. Di(2-ethylhexyl) phthalate (DEHP), an endocrine disrupting chemical in PM2.5, has been utilized for the manufacturing of polyvinyl chloride to increase the flexibility of final products. In the present study, we investigated the ecotoxicological effect of DEHP on the viability of skin keratinocytes (HaCaT). DEHP induced apoptotic cell death mediated by phosphorylation of extracellular signal-regulated kinase through the production of intracellular Reactive Oxygen Species (ROS). Interestingly, we found that DEHP induces the phosphorylation of the nuclear factor-kappa B responsible for the expression of cleaved caspase-3 as an executional cell death protease in HaCaT cells. On the basis of these results, we suggest that DEHP in PM2.5 induces the apoptotic death of human keratinocytes via ROS-mediated signaling events.

Acknowledgement

Supported by : 한국연구재단

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