Effect of Growth Regulator, Sucrose, and Minimal-growth Conservation on In Vitro Propagation of Virus-free Sweet Potato Plantlets

고구마 무병묘의 기내 증식에 미치는 생장조절물질, Sucrose, 최소생장 보존의 영향

  • Lee, Na Rha (Institute of Life Science and Natural Resources, Wonkwang University) ;
  • Lee, Seung Yeob (Institute of Life Science and Natural Resources, Wonkwang University)
  • 이나라 (원광대학교 생명자원과학연구소) ;
  • 이승엽 (원광대학교 생명자원과학연구소)
  • Received : 2019.07.04
  • Accepted : 2019.11.06
  • Published : 2020.01.31


The influence of growth regulators (NAA and BA) and sucrose concentrations (0, 3, 5, 7, 9%) on in vitro rapid-propagation of virus-free sweet potato [Ipomoea batatas (L.) Lam.] was investigated with single-node or shoot-tip culture of two cultivars ('Matnami' and 'Shinhwangmi'). The survival rate and growth of shoot-tip explant was also investigated under the presence or absence of light (blue and red LED = 7:3, 150±5 μmol·m-2·s-1 PPFD) during minimal-growth in vitro conservation at 15℃. Vine length, vine diameter, fresh weight and dry weight were enhanced without callusing of explant in the MS medium supplemented with 0.2-0.5 mg·L-1 BA. The growth of single-node and shoot-tip explants were significantly enhanced with the increase of vine length, number of leaf, number of root, fresh weight, and dry weight in the solid medium containing 5% sucrose and 0.2 mg·L-1 BA. Vine elongation of shoot-tip explants were highest in the liquid medium containing 3% sucrose than the solid medium. The survival rate of minimal-growth in vitro conservation was 100% in 5 months under the presence of light (LED, 150±5 μmol·m-2·s-1 PPFD) at 15℃, but the explants in dark condition died in 3 months. The light was absolutely necessary for the in vitro conservation under minimal-growth conditions of virus-free sweet potato plantlets at 15℃, and the high density of explants (10 plantlets per Petri Dish) was increased the efficiency of mass conservation.


Supported by : 원광대학교


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