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Effect of Growth Regulator, Sucrose, and Minimal-growth Conservation on In Vitro Propagation of Virus-free Sweet Potato Plantlets

고구마 무병묘의 기내 증식에 미치는 생장조절물질, Sucrose, 최소생장 보존의 영향

  • Lee, Na Rha (Institute of Life Science and Natural Resources, Wonkwang University) ;
  • Lee, Seung Yeob (Institute of Life Science and Natural Resources, Wonkwang University)
  • 이나라 (원광대학교 생명자원과학연구소) ;
  • 이승엽 (원광대학교 생명자원과학연구소)
  • Received : 2019.07.04
  • Accepted : 2019.11.06
  • Published : 2020.01.31

Abstract

The influence of growth regulators (NAA and BA) and sucrose concentrations (0, 3, 5, 7, 9%) on in vitro rapid-propagation of virus-free sweet potato [Ipomoea batatas (L.) Lam.] was investigated with single-node or shoot-tip culture of two cultivars ('Matnami' and 'Shinhwangmi'). The survival rate and growth of shoot-tip explant was also investigated under the presence or absence of light (blue and red LED = 7:3, 150±5 μmol·m-2·s-1 PPFD) during minimal-growth in vitro conservation at 15℃. Vine length, vine diameter, fresh weight and dry weight were enhanced without callusing of explant in the MS medium supplemented with 0.2-0.5 mg·L-1 BA. The growth of single-node and shoot-tip explants were significantly enhanced with the increase of vine length, number of leaf, number of root, fresh weight, and dry weight in the solid medium containing 5% sucrose and 0.2 mg·L-1 BA. Vine elongation of shoot-tip explants were highest in the liquid medium containing 3% sucrose than the solid medium. The survival rate of minimal-growth in vitro conservation was 100% in 5 months under the presence of light (LED, 150±5 μmol·m-2·s-1 PPFD) at 15℃, but the explants in dark condition died in 3 months. The light was absolutely necessary for the in vitro conservation under minimal-growth conditions of virus-free sweet potato plantlets at 15℃, and the high density of explants (10 plantlets per Petri Dish) was increased the efficiency of mass conservation.

Acknowledgement

Supported by : 원광대학교

References

  1. Alam I., S.A. Sharmin, M.K. Naher, M.J. Alam. M. Anisuzzaman, and M.F. Alam. 2010. Effect of growth regulators on meristem culture and plantlet establishment in sweet potato [Ipomoea Batatas (L.) Lam.]. POJ 3:35-39.
  2. Alam I., S.A. Sharmin, M.K. Naher, M.J. Alam, M. Anisuzzaman, and M.F. Alam. 2013. Elimination and detection of viruses in meristem-derived plantlets of sweet potato as a low-cost option toward commercialization. 3 Biotech 3:153-164.
  3. Carswell, G.K. and R.D. Locy. 1984. Root and shoot initiation by leaf, stem, and storage root explants of sweet potato. Plant Cell Tiss. Org. Cult. 3:229-236. https://doi.org/10.1007/BF00040342
  4. Eun, J.S., J.S. Park, and Y.S. Kim. 2002. Effects of cultural conditions on the in vitro propagation of plantlets derived from apical meristem in Ipomoea batatas L. J. Plant Biotechnol. 29:37-40 (in Korean). https://doi.org/10.5010/JPB.2002.29.1.037
  5. Karim, M.Z., S. Yokota, M.M. Rahman, J. Eizawa, Y. Saito, M.A.K. Azad, F. Ishiguri, K. Iizuka, and N. Yoshizawa. 2007. Effect of the sucrose concentration and pH level on shoot regeneration from callus in Araria elata Seem. Asian J. Plant Sci. 6:715-717. https://doi.org/10.3923/ajps.2007.715.717
  6. Karyeija, R.F., R.W. Gibson, and J.P.T. Valkonen. 1998. The significance of sweetpotato feathery mottle virus in subsistence sweetpotato production in Africa. Plant Dis. 82:4-15. https://doi.org/10.1094/PDIS.1998.82.1.4
  7. Kovalchuk, I., Y. Lyudvikova, M. Volgina, and B.M. Reed. 2009. Medium, container and genotype all influence in vitro cold storage of apple germplasm. Plant Cell Tiss. Org. Cult. 96:127-136. https://doi.org/10.1007/s11240-008-9468-8
  8. Gopal, J. and N.S. Chauhan. 2010. Slow growth in vitro conservation of potato germplasm at low temperature. Potato Res. 53:141-149. https://doi.org/10.1007/s11540-010-9158-x
  9. Lee S.Y. and T.O. Kwon. 2013. Factors affecting in vitro minimal growth conservation of Sedum sarmentosum. Protected Hort. Plant Fac. 22:241-247 (in Korean). https://doi.org/10.12791/KSBEC.2013.22.3.241
  10. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant 15:473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  11. Nam S.S., M.N. Chung, J.S. Lee, H.U. Lee, J.W. Yang, E.J. Hwang, and K.B. Lee. 2016. Effect of liquid culture on the propagation of virus free plantlets from meristem culture-derived of sweetpotato [Ipomoea batatas L. (Lam)]. J. Korean Soc. Int. Agric. 28:215-219 (in Korean). https://doi.org/10.12719/KSIA.2016.28.2.215
  12. Ndagijimana V., J. Kahia, T. Asiimwe, P.Y. Sallah, B. Waweru, I. Mushimiyimana, J. Ndiriwe, Sindi Kirimi, D. Shumbusha. P. Njena, M. Kouassi, and E. Koffi. 2014. In vitro effects of gibberellic acid and sucrose concentration on mocropropagation of two elite sweet potato cultivars in Rwanda. Int. J. Biotechnol. Mol. Biol. Res. 5:1-6.
  13. Nowak, B., K. Miczynski, and L. Hudy. 2004. Sugar uptake and utilization during adventitious bud differentiation on in vitro leaf explants of 'Wegierka Zwykla' plum (Prunus domestica). Plant Cell Tiss. Org. Cult. 76:255-260. https://doi.org/10.1023/B:TICU.0000009247.94819.02
  14. Reed, B.M. 1992. Cold storage of strawberries in vitro: a comparison of three storage systems. Fruit Var. J. 46:98-102.
  15. Santa-Maria, M., K.V. Pecota, C.G. Yencho, G. Allen, and B. Sosinski. 2009. Rapid shoot regeneration in industrial 'high starch' sweetpotato (Ipomoea batatas L.) genotypes. Plant Cell Tiss. Org. Cult. 97:109-117. https://doi.org/10.1007/s11240-009-9504-3
  16. Sarkar, D. and P.S. Naik. 1998. Factors affecting minimal growth conservation of potato microplants in vitro. Euphytica 102:275-280. https://doi.org/10.1023/A:1018309300121
  17. Teow, C.C., V. Truong, R.F. McFeeters, R.L. Thompson, K.V. Pecota, and G.C. Yencho. 2007. Antioxidant activities, phenolic and $\beta$-carotene contents of sweet potato genotypes with varying flesh colours. Food Chem. 103:829-838. https://doi.org/10.1016/j.foodchem.2006.09.033
  18. Westcott, R.J. 1981. Tissue culture storage of potato germplasm. 1. Minimal growth storage. Potato Research 24:331-342. https://doi.org/10.1007/BF02360370
  19. Yang, X. 2010. Rapid production of virus-free plantlets by shoot tip culture in vitro of purple-coloured sweet potato (Ipomoea batatas (L.) LAM.). Pak. J. Bot., 42: 2069-2075.
  20. Yoo K.R. and S.Y. Lee. 2013. Growth characteristics and yield of sweet potato cultivars between virus-free and farmer's slips in late season cultivation. Kor. J. Crop Sci. 58:43-49 (in Korean). https://doi.org/10.7740/kjcs.2013.58.1.043
  21. Yoo, K.R. and S.Y. Lee. 2017. Effects of light-emitting diodes on in vitro growth of virus-free sweet potato plantlets. Kor. J. Hortic. Sci. Technol. 35:490-498 (in Korean).