A report of 35 unreported bacterial species in Korea, belonging to the phylum Firmicutes

  • Baek, Min-gyung (Department of Public Health Science, Korea University) ;
  • Kim, Wonyong (Department of Microbiology, Chung-Ang University College of Medicine) ;
  • Cha, Chang-Jun (Department of Biotechnology, Chung-Ang University) ;
  • Joh, Kiseong (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Kim, Seung-Bum (Department of Microbiology, Chungnam National University) ;
  • Kim, Myung Kyum (Department of Bio and Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Seong, Chi-Nam (Department of Biology, Sunchon National University) ;
  • Yi, Hana (Department of Public Health Science, Korea University)
  • 투고 : 2019.09.20
  • 심사 : 2019.10.18
  • 발행 : 2019.11.30


In an investigation of indigenous prokaryotic species in Korea, a total of 35 bacterial strains assigned to the phylum Firmicutes were isolated from diverse habitats including natural and artificial environments. Based on their high 16S rRNA gene sequence similarity (>98.7%) and formation of robust phylogenetic clades with species of validly published names, the isolates were identified as 35 species belonging to the orders Bacillales (the family Bacillaceae, Paenibacillaceae, Planococcaceae, and Staphylococcaceae) and Lactobacillales (Aerococcaceae, Enterococcaceae, Lactobacillaceae, Leuconostocaceae, and Streptococcaceae). Since these 35 species in Korean environments has not been reported in any official report, we identified them as unrecorded bacterial species and investigated them taxonomically. The newly found unrecorded species belong to 20 species in the order Bacillales and 15 species in the order Lactobacillales. The morphological, cultural, physiological, and biochemical properties of the isolates were examined and the descriptive information of the 35 previously unrecorded species is provided here.


연구 과제번호 : The Survey of Korean Indigenous Species

연구 과제 주관 기관 : National Institute of Biological Resources


  1. Backhed, F., H. Ding, T. Wang, L.V. Hooper, G.Y. Koh, A. Nagy and J.I. Gordon. 2004. The gut microbiota as an environmental factor that regulates fat storage. Proceedings of the National Academy of Sciences. USA 10144:15718-15723.
  2. Gibbons, N. and R. Murray. 1978. Proposals concerning the higher taxa of bacteria. International Journal of Systematic and Evolutionary Microbiology 28:1-6.
  3. Jeffery, I.B., P.W. O'toole, L. Ohman, M.J. Claesson, J. Deane, E.M. Quigley and M. Simren. 2012. An irritable bowel syndrome subtype defined by species-specific alterations in faecal microbiota. Gut 61:997-1006.
  4. Jeon, Y.-S., K. Lee, S.-C. Park, B.-S. Kim, Y.-J. Cho, S.-M. Ha and J. Chun. 2014. EzEditor: a versatile sequence alignment editor for both rRNA- and protein-coding genes. International Journal of Systematic and Evolutionary Microbiology 64:689-691.
  5. Kumar, S., G. Stecher and K. Tamura. 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33:1870-1874.
  6. Ley, R.E., F. Backhed, P. Turnbaugh, C.A. Lozupone, R.D. Knight and J.I. Gordon. 2005. Obesity alters gut microbial ecology. Proceedings of the National Academy of Sciences. USA 102:11070-11075.
  7. Rajilic-Stojanovic, M., E. Biagi, H.G. Heilig, K. Kajander, R.A. Kekkonen, S. Tims and W.M. de Vos. 2011. Global and deep molecular analysis of microbiota signatures in fecal samples from patients with irritable bowel syndrome. Gastroenterology 141:1792-1801.
  8. Turnbaugh, P.J., M. Hamady, T. Yatsunenko, B.L. Cantarel, A. Duncan, R.E. Ley and J.P. Affourtit. 2009. A core gut microbiome in obese and lean twins. Nature 457:480-484.
  9. De Vos, P., G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.A. Rainey and W.B. Whitman. 2011. Bergey's manual of systematic bacteriology: Volume 3: The Firmicutes (Vol. 3): Springer Science & Business Media.
  10. Yoon, S.-H., S.-M. Ha, S. Kwon, J. Lim, Y. Kim, H. Seo and J. Chun. 2017. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. International Journal of Systematic and Evolutionary Microbiology 67:1613-1617.