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Elizabethkingia miricola BM10, a New Symbiotic Bacterium Isolated from the Hindgut of the Termite Reticulitermes speratus KMT001

  • LEE, Dongmin (Department of Forest Products and Biotechnology, College of Science and Technology, Kookmin University) ;
  • KIM, Young-Kyoon (Department of Forest Products and Biotechnology, College of Science and Technology, Kookmin University) ;
  • KIM, Yeong-Suk (Department of Forest Products and Biotechnology, College of Science and Technology, Kookmin University) ;
  • KIM, Tae-Jong (Department of Forest Products and Biotechnology, College of Science and Technology, Kookmin University)
  • Received : 2019.03.01
  • Accepted : 2019.10.18
  • Published : 2019.11.25

Abstract

Elizabethkingia miricola BM10, a symbiotic bacterium, has been isolated from the hindgut of Reticulitermes speratus KMT001, a termite which occurs on Bukhan Mountain in Seoul, Korea. This strain demonstrated a symbiotic characteristic, in that it lacked endo-${\beta}$-1,4-glucanase activity, in a previous study. The major fatty acids of E. miricola BM10 were iso-$C_{15:0}$, iso-$C_{17:0}$ 3-OH, and summed feature 3 (iso-$C_{16:1}{\omega}7c/C_{16:1}{\omega}6c$). The content of iso-$C_{17:0}$ 3-OH was higher, while those of ECL 13.566, iso-$C_{17:11}{\omega}9c$, and summed feature 4 were lower than the other three type-strains of the Elizabethkingia genus. The 16S rRNA phylogenetic analysis confirmed that E. miricola BM10 is a new species. The whole genome of E. miricola BM10 was sequenced. The average nucleotide identity of strain BM10 as evaluated by pairwise comparison with E. anophelis R26, E. meningoseptica ATCC 13253, and E. miricola GTC 862 was shown to be 91.5%, 81.2%, and 94.29%, respectively. Based on our study results, E. miricola BM10 appears to represent a new strain of the genus Elizabethkingia.

Keywords

Elizabethkingia miricola;termite;symbiotic bacteria;hindgut;new strain

Acknowledgement

Supported by : Korea Forestry Promotion Institute

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