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Effects of Lentils(Lens culinaris) Extract on Proteasome Activity and Nuclear Factor κB Activation in HepG2 Human Liver Cancer Cells

렌즈콩(Lens culinaris) 추출물이 HepG2 인간 간암 세포에서 Proteasome 활성과 Nuclear Factor κB 활성화에 미치는 영향

  • Min, Sooyeong (Dept. of Food and Nutrition, Sungshin Women's University) ;
  • Yoon, Hyungeun (Dept. of Food Science and Biotechnology, Sungshin Women's University)
  • 민수영 (성신여자대학교 식품영양학과 대학원) ;
  • 윤현근 (성신여자대학교 바이오식품공학과)
  • Received : 2019.08.21
  • Accepted : 2019.09.18
  • Published : 2019.10.31

Abstract

Proteasome inhibitors can improve the efficiency of cancer treatments by inhibiting nuclear factor ${\kappa}B$($NF-{\kappa}B$) activation in cancer cells. Lentils are a type of beans of which consumption of such beans is increasing. The purpose of this study was to investigate the effects of lentils extract (LE) on the proteasomal activities, $NF-{\kappa}B$ activation, and cell cycle in HepG2 human liver cancer cells. LE treatments inhibited proteasomal activities at concentrations of 10, 50, and $100{\mu}g/mL$ respectively, and repressed $NF-{\kappa}B$ activation at concentrations of 1, 10, and $100{\mu}g/mL$ respectively, in HepG2 cells. LE treatments at concentrations of 1, 10, and $100{\mu}g/mL$ respectively, increased sub-G1 cell population in HepG2 cells, which may be the result of apoptosis. The results suggest that LE inhibited $NF-{\kappa}B$ activation partially with its proteasome inhibitory activities, and the increase of sub-G1 cell population was induced partially, by inhibition of $NF-{\kappa}B$ activation in HepG2 cells.

Keywords

proteasome inhibitor;nuclear factor ${\kappa}B$;lentils extract;HepG2 cells

Acknowledgement

Supported by : 한국연구재단

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