Effects of Lentils(Lens culinaris) Extract on Proteasome Activity and Nuclear Factor κB Activation in HepG2 Human Liver Cancer Cells

렌즈콩(Lens culinaris) 추출물이 HepG2 인간 간암 세포에서 Proteasome 활성과 Nuclear Factor κB 활성화에 미치는 영향

  • Min, Sooyeong (Dept. of Food and Nutrition, Sungshin Women's University) ;
  • Yoon, Hyungeun (Dept. of Food Science and Biotechnology, Sungshin Women's University)
  • 민수영 (성신여자대학교 식품영양학과 대학원) ;
  • 윤현근 (성신여자대학교 바이오식품공학과)
  • Received : 2019.08.21
  • Accepted : 2019.09.18
  • Published : 2019.10.31


Proteasome inhibitors can improve the efficiency of cancer treatments by inhibiting nuclear factor ${\kappa}B$($NF-{\kappa}B$) activation in cancer cells. Lentils are a type of beans of which consumption of such beans is increasing. The purpose of this study was to investigate the effects of lentils extract (LE) on the proteasomal activities, $NF-{\kappa}B$ activation, and cell cycle in HepG2 human liver cancer cells. LE treatments inhibited proteasomal activities at concentrations of 10, 50, and $100{\mu}g/mL$ respectively, and repressed $NF-{\kappa}B$ activation at concentrations of 1, 10, and $100{\mu}g/mL$ respectively, in HepG2 cells. LE treatments at concentrations of 1, 10, and $100{\mu}g/mL$ respectively, increased sub-G1 cell population in HepG2 cells, which may be the result of apoptosis. The results suggest that LE inhibited $NF-{\kappa}B$ activation partially with its proteasome inhibitory activities, and the increase of sub-G1 cell population was induced partially, by inhibition of $NF-{\kappa}B$ activation in HepG2 cells.


proteasome inhibitor;nuclear factor ${\kappa}B$;lentils extract;HepG2 cells


Supported by : 한국연구재단


  1. Cusack JC, Liu R, Houston M, Abendroth K, Elliott PJ, Adams J, Baldwin AS. 2001. Enhanced chemosensitivity to CPT-11 with proteasome inhibitor PS-341: Implications for systemic nuclear factor-${\kappa}B$ inhibition. Cancer Res 61:3535-3540
  2. Darzynkiewicz Z, Juan G. 2001. DNA content measurement for DNA ploidy and cell cycle analysis. Curr Protoc Cytom Chapter 1:7.5.1-7.5.24
  3. De Bettignies G, Coux O. 2010. Proteasome inhibitors: Dozens of molecules and still counting. Biochimie 92:1530-1545
  4. Flankland-Searby S, Bhaumik SR. 2012. The 26S proteasome complex: An attractive target for cancer therapy. Biochim Biophys Acta 1825:64-76
  5. Ganesan K, Xu B. 2017. Polyphenol-rich lentils and their health promoting effects. Int J Mol Sci 18:1-23
  6. Hideshima T, Chauhan D, Richardson P, Mitsiades C, Mitsiades N, Hayashi T, Munshi N, Dang L, Castro A, Palombella V, Adams J, Anderson KC. 2002. NF-${\kappa}B$ as a therapeutic target in multiple myeloma. J Biol Chem 277:16639-16647
  7. Im JY, Kim SC, Kim S, Choi Y, Yang MR, Cho IH, Kim HR. 2016. Protein and amino-acid contents in Backtae, Seoritae, Huktae, and Seomoktae soybeans with different cooking methods. Korea J Food Cookery Sci 32:567-574
  8. Jameel M, Ali A, Ali M. 2015. Isolation of antioxidant phytoconstituents from the seeds of Lens culinaris Medik. Food Chem 175:358-365
  9. Lee KH. 2015. Antioxidant component and sensory evaluation of mixed cereals. Korean J Food Nutr 28:196-201
  10. Lee S, Lee YB, Kim HS. 2013. Analysis of the general and functional components of various soybeans. J Korean Soc Food Sci Nutr 42:1255-1262
  11. Liu M, Li XQ, Weber C, Lee CY, Brown J, Liu RH. 2002. Antioxidant and antiproliferative activities of raspberries. J Agric Food Chem 50:2926-2930
  12. Ludwig H, Khayat D, Gaccone G, Facon T. 2005. Proteasome inhibition and its clinical prospects in the treatment of hematologic and solid malignancies. Cancer 104:1794-1807
  13. Manasanch EE, Orlowski RZ. 2017. Proteasome inhibitors in cancer therapy. Nat Rev Clin Oncol 14:417-433
  14. Richardson PG, Kumar S, Laubach JP, Paba-Prada C, Gupta N, Berg D, van de Velde H, Moreau P. 2017. New developments in the management of relapsed/refractory multiple myeloma - the role of ixazomib. J Blood Med 8:107-121
  15. Song JE, Song JH, Cho SM, Min GH, Lee JS. 2010. Nutritional characteristics and physiological functionality of antidementia acetylcholinesterase inhibitor-containing methanol extract from Sorghum bicolor. Korean J Food Nutr 23:226-232
  16. Yoon H, Lee CY. 2010. Effect of selected phytochemicals on cell proliferation in A549 lung cancer cells. Food Sci Biotechnol 19:1063-1068
  17. Yoon H, Liu RH. 2007. Effect of selected phytochemicals and apple extracts on NF-kappaB activation in human breast cancer MCF-7 cells. J Agric Food Chem 55:3167-3173