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Evaluation of Antibacterial, Antioxidant Fractionalities and Hair Loss Prevention Effect of Platycodon grandiflorum

도라지 추출성분의 항균·항산화 및 탈모예방 효과

  • Jung, Min-Hwa (Hair Scalp Research Institute in Daedong College)
  • 정민화 (대동대학교 두피.모발연구소)
  • Received : 2019.04.26
  • Accepted : 2019.10.18
  • Published : 2019.10.31

Abstract

To investigate the effects of antioxidant activities and hair loss prevention of extracts from Platycodon grandiflorum, we've prepared chloroform (CF) and ethylacetate fractions (EA) extracted from P. grandiflorum. In the results of DPPH radical scavenging assay, the two fractions showed dose-dependent antioxidant activities. Furthermore, in the ABTS assay, the two fractions exhibited the inhibitory effect over 90% at 10, 50, 100, 200 mg/mL. To investigate the inflammation inhibitory effect, we used RAW264.7 cells, these extracts were inhibited inflammatory reaction by suppressing the production of nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) in dose-dependent manner. In the assay of HaCaT cells' proliferation, the 24 hr treatment of the extracts significantly accelerarted cell proliferation in the range of concentrations used. The two fractions inhibited the proliferation of Malssezia furfur, the bacteria induce the dandruff. Finally, the CF could significantly inhibit the DHT production at 1, 10, 50, $100{\mu}g/mL$, but EA showed the inhibitory effect at the concentration over $50{\mu}g/mL$. The overall results of this study suggest that the chloroform (CF) and ethylacetate fractions (EA) from P. grandiflorum could be a useful raw material for the hair loss prevention products.

Keywords

Platycodon grandiflorum;NO;$PGE_2$;DPPH;DHT

Acknowledgement

Supported by : 대동대학교

References

  1. An GS, Hwang IC. 2009. Hair loss prevention and hair growth promotion by herb extracts which contain the cultured Korean wild ginseng. J Korean Soc Beauty Art 10:221-226
  2. Boo HO, Park JH, Kim HH, Kwon SJ, Woo SH. 2018. Evaluation of physiological functionalities and anti-inflammatory activity on in vitro cultured adventitious root of Platycodon grandiflorum. J Corp Sci Biotech 21:183-191 https://doi.org/10.1007/s12892-018-0101-0
  3. Jeong CH, Choi GN, Kim JH, Kwak JH, Kim DO, Kim YJ, Heo HJ. 2009. Antioxidant activities from the aerial parts of Platycondon grandiflorum. Food Chem 118:278-282
  4. Jung SY. 2016. Anti-microbial activity of Platycodon grandiflorum extracts against oral microbes. Master's Thesis, Yeungnam Univ. Gyeongsan, Korea
  5. Kawano M, Han J, Kchouk ME, Isoda H. 2009. Hair growth regulation by the extract of aromatic plant Erica multiflora. J Nat Med 63:335-339 https://doi.org/10.1007/s11418-009-0324-x
  6. Kim GR. 2011. A study on the effect of Eclipta prostrata extract and MTS on the improvement of scalp health and prevention of hair loss for workers in their 20s and 30s. Master's Thesis, Konkuk Univ. Seoul, Korea
  7. Kim J. 2014. Antibacterial and anti-inflammatory effects of Platycodon grandiflorum extracts. J Digital Converg 12:359-366
  8. Lee JL, Im EJ. 2009. Analysis and forecast of the domestic market for hair loss. Korean J Aesthet Cosmetol 7:155-163
  9. Lee TH, Kwak HB, Kim HH, Lee ZH, Chung DK, Baek NI, Kim J. 2007. Methanol extracts of stewartia koreana inhibit cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) gene expression by blocking NF-kappaB transactivation in LPS-activated RAW 264.7 cells. Mol Cells 23:398-404
  10. Leng J, Wang Z, Fu CL, Zhang J, Ren S, Hu JN, Jiang, S, Wang YP, Chen C, Li W. 2018. NF-${\kappa}B$ and AMPK/PI3K/Akt signaling pathways are involved in the protective effects of Platycodon grandiflorum saponins against acetaminopheninduced acute hepatotoxicity in mice. Phytother Res 32:2235-2246 https://doi.org/10.1002/ptr.6160
  11. Liu F, Miao Y, Li X, Qu Q, Liu Y, Li K, Feng C, Hu Z. 2018. The relationship between self-esteem and hair transplantation satisfaction in male androgenetic alopecia patients. J Cosmet Dermatol 18:1-7
  12. Madaan A, Joshi V, Kishore A, Verma R, Singh AT, Jaggi M, Sung YK. 2017. In vitro hair growth promoting effects of naringenin and hesperetin on human dermal papilla cells and keratinocytes. Am J Dermatol Venereol 6:51-57
  13. Moreno-Arrones OM, Becerra A, Vano-Galvan S. 2017. Therapeutic experience with oral finasteride for androgenetic alopecia in female-to-male transgender patients. Clin Exp Dermatol 42:743-748 https://doi.org/10.1111/ced.13184
  14. Park SJ, Kim JW, Park SJ, Kim TJ. 2012. Effects of Platycodon grandiflorum including platycodin D in IgE/Ag-induced type I hypersensitivity. J Life Sci 22:595-599 https://doi.org/10.5352/JLS.2012.22.5.595
  15. Patal S, Sharma V, Chauhan NS, Thakur M, Dixit VK. 2015. Hair growth: Focus on herbal therapeutic agent. Curr Drug Discov Technol 12:21-42 https://doi.org/10.2174/1570163812666150610115055
  16. Pekmezci E, Turkoglu M, Gokalp H, Kutlubay Z. 2018. Minoxidil downregulates interleukin-1 alpha gene expression in HaCaT cells. Int J Trichology 10:108-112 https://doi.org/10.4103/ijt.ijt_18_17
  17. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans R. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  18. Stenn KS, Paus R. 2001. Controls of hair follicle cycling. Physiol Rev 81:449-494 https://doi.org/10.1152/physrev.2001.81.1.449
  19. Vane JR. 1971. Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs. Nat New Biol 231:232-235 https://doi.org/10.1038/newbio231232a0
  20. Woo H, Kim Y, Lee Y, Kim IH, Kim SJ. 2018. Melatonin content and antioxidant activity of Platycodon grandiflorum seed extract. J Korean Soc Food Sci Nutr 47:876-884 https://doi.org/10.3746/jkfn.2018.47.9.876
  21. Yi EY, Kim YJ. 2017. Extract of balloon-flower inhibited in vitro angiogenesis in human umbilical vein endothelial cells. J Life Sci 27:1059-1063
  22. Yoshida T, Mori K, Hatano T, Okumura T, Uehara Y, Komagoe K, Fujita Y, Okuda T. 1989. Studies on inhibition mechanism of autoxidation by tannins and flavonoids. V Radical-scavenging effects of tannins and related polyphenols on 1, 1-diphenyl-2-picrylhydrzyl radical. Chem Pharm Bull 37:1919-1921 https://doi.org/10.1248/cpb.37.1919