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Enzyme Inhibitory and Anti-Proliferation Effects of Peanut Skin Extracts Depending on Cultivar

품종별 땅콩 종피 추출물의 효소저해활성 및 암세포주 증식억제 효과

  • Kim, Min Young (Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Hyun-Joo (Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Yu-Young (Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Mi Hyang (Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Jin Young (Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Byoungkyu (Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Byong Won (Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration)
  • 김민영 (국립식량과학원 중부작물부 수확후이용과) ;
  • 김현주 (국립식량과학원 중부작물부 수확후이용과) ;
  • 이유영 (국립식량과학원 중부작물부 수확후이용과) ;
  • 김미향 (국립식량과학원 중부작물부 수확후이용과) ;
  • 이진영 (국립식량과학원 중부작물부 수확후이용과) ;
  • 이병규 (국립식량과학원 중부작물부 수확후이용과) ;
  • 이병원 (국립식량과학원 중부작물부 수확후이용과)
  • Received : 2019.09.19
  • Accepted : 2019.10.14
  • Published : 2019.10.31

Abstract

The purpose of this study was to investigate the inhibitory effect of enzyme activity and anti-proliferation of human cancer cell lines (HCT 116, NCI-H460 and MCF-7) of peanut skin depending on cultivars (Arachis hypogaea L. cv. K-Ol, cv. Sinpalkwang, cv. Daan, cv. Heuksaeng) and extraction solvent. Peanut skin was extracted with 80% ethanol, 80% methanol, 80% acetone, and distilled water, followed by analysis of the enzyme inhibitory activity and anticancer activity. Methanol extract of Daan cultivar most effectively inhibited ${\alpha}$-gluosidase (65.08%, 0.025 mg/mL), tyrosinase (82.49%, 2 mg/mL) and ACE (73.61%, 10 mg/mL). The inhibitory effect of peanut skin extracts on colon cancer cell (HCT-116), lung cancer cell (NCI-H460) and breast cancer cell (MCF-7) growth were investigate using MTT assay. The highest anti-proliferation of cancer cell line of peanut skin extracts was observed in the methanol extract of Daan cultivar. The cell viability on HCT 116, NCI-H460 and MCF-7 cell lines of methanol extracts from peanut skin of Daan cultivar was 48.13%, 41.03%, and 36.02% at $200{\mu}g/mL$, respectively. These results suggest that peanut skin extracts may mediate physiological activity, and provide valuable information for the use of peanut byproduct as a functional food material.

Keywords

peanut skin;cultivars;enzyme inhibitory activity;anticancer activity

Acknowledgement

Grant : 국내 육성땅콩의 생리활성 검정

Supported by : 농촌진흥청

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