Properties of Amino Acid and Volatile Flavor Compounds of Fermented Soybean Products by Soybean Cultivar

콩 품종에 따른 발효물의 아미노산과 향기성분 특성

  • Shin, Dong Sun (Dept. of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Choi, In Duck (Dept. of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Seuk Ki (Dept. of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Park, Ji Young (Dept. of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Nam Geol (Dept. of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Park, Chang Hwan (Dept. of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Choi, Hye Sun (Dept. of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration)
  • 신동선 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 최인덕 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 이석기 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 박지영 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 김남걸 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 박장환 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 최혜선 (농촌진흥청 국립식량과학원 중부작물부)
  • Received : 2019.06.21
  • Accepted : 2019.09.18
  • Published : 2019.10.31


In this study, we analyzed the nutritional composition properties of soybeans and the organic acids, amino acids and volatile flavor compounds of fermented soybean products. We used five soybean cultivars including Pyeongwon, Jinpung, Saedanbaek, Saeolkong and Cheonga for this experiment. Physicochemical analysis of soybeans, showed that the cured protein and fat contents were 35.12~45.12 and 14.26~20.14%, respectively. The rank order of major organic acids was lactic acid > acetic acid > fumaric acid, with Saedanbaek being the highest. Total amino acid content of the samples was 358.12~657.28 mg/100 g, and glutamic acid, alanine, cysteine, valine, leucine, histidine and arginine were the major amino acids. We identified a total of 34 volatile aroma-compounds, including 7 alcohols, 7 acids, 7 ketones, 5 phenols, 2 esters, 1 furan, 4 pyrazines, and 1 miscellaneous compounds. As a result of this, could be applied to determine the suitability of cultivars and the quality for the process of the fermented soybean products.


soybean;cultivars;fermentation;amino acids;volatile compounds


Supported by : 농촌진흥청


  1. Akitha MK, Gondi M, Sakthivelu G, Giridhar P, Rajasekaran T, Ravishankar GA. 2009. Functional attributes of soybean seeds and products with reference to isoflavone content and antioxidant activity. Food Chem 114:771-776
  2. Allagheny N, Obanu ZA, Campbell-Platt G, Owens JD. 1996. Control of ammonia formation during Bacillus subtilis fermentation of legumes. Int J Food Microbiol 29:321-333
  3. Baek IY, Ko JM, Kang ST, Han WY, Shin DC, Oh SK, Chung MG, Kim HT, Kang NS, Oh KW, Shin SO, Park KY, Suh DY, Yun HT, Oh YJ, Lee YS, Son CK, Kim YD, Kim HY, Lee SH. 2006. A new vegetable soybean cultivar, 'Danmi 2' with free of lioxygenase 1 and 2. Korean J Breed 38:301-302
  4. Cao ZH, Green-Johnson JM, Buckley ND, Lin QY. 2019. Bioactivity of soy-based fermented foods: A review. Biotechnol Adv 37:223-238
  5. Cassileth BR, Vickers AJ. 2003. Soy: An anticancer agent in wide use despite some troubling data. Cancer Invest 21:817-818
  6. Cho YJ, Cha WS, Bok SK, Kim MU, Chun SS, Choi UK. 2000. Production and separation of anti-hypertensive peptide during chunggugjang fermentation with Bacillus subtilis CH-1023. J Korean Soc Agric Chem Biotechnol 43:247-252
  7. Choi JH, Lee M, Kim HJ, Kwon JI, Lee Y. 2017. Effects of black soybean and fermented black soybean extracts on proliferation of human follicle dermal papilla cells. J Korean Soc Food Sci Nutr 46:671-680
  8. Eom SM, Jung BY, Oh HI. 2009. Changes in chemical components of cheonggukjang prepared with germinated soybeans during fermentation. J Appl Biol Chem 52:133-141
  9. Gil NY, Song J, Eom JS, Park SY, Choi HS. 2016. Changes of physicochemical properties of Cheonggukjang prepared with various soybean cultivars and Bacillus subtilis HJ18-9. Korean J Food Preserv 23:811-818
  10. Ha KS, Choi JK, Heo NK, Kim SS, Lee AS, Jang JS, Yun HT. 2013. A new tofu and soy-paste soybean cultivar 'Hoban' with large seed and high yield. Korean J Breed Sci 45:158-162
  11. Ito T, Sugawara E, Mitanogara T, Sakuri Y, Odagiri S. 1989. Effect of amino acids as nitrogen sources on microbiological formation of pyrazines. Nippon Shokuhim Kogyo Gakkaishi 36:762-764
  12. Jung TD, Shin GH, Kim JM, Oh JW, Choi SI, Lee JH, Lee SJ, Heo IY, Park SJ, Kim HT, Kang BK, Lee OH. 2016. Assessment of validation method for bioactive contents of fermented soybean extracts by bioconversion and their antioxidant activities. J Korean Soc Food Sci Nutr 45:680-689
  13. Kim HT, Ko JM, Baek IY, Jeon MK, Han WY, Park KY, Lee BW, Lee YH, Jung CS, Oh KW, Ha TJ, Moon JK, Yun HT, Lee JH, Choi JK, Jung JH, Lee SS, Jang YJ, Son CK, Kang DS. 2014. Soybean cultivar for tofu, 'Saedanbaek' with disease resistance, and high protein content. Korean J Breed Sci 46:295-301
  14. Kim IJ, Kim HK, Chung JH, Jeong YK, Ryu CH. 2002. Study of functional chungkukjang contain fibrinolytic enzyme. Korean J Life Sci 12:357-362
  15. Kim MY, Kim M, Hwang JH, Kim SH, Jeong YJ. 2017. Comparison of quality characteristics of doenjang reduced of sodium content. Korean J Food Preserv 24:771-777
  16. Kim SH, Yang JL, Song YS. 1999. Physiological functions of Chongkukjang. Food Industry Nutr 4:40-46
  17. Kim YS, Kim MC, Kwon SW, Kim SJ, Park IC, Ka JO, Weon HY. 2011. Analyses of bacterial communities in meju, a Korean traditional fermented soybean bricks, by cultivation-based and pyrosequencing methods. J Microbiol 49:340-348
  18. Lee SY, Eom JS, Choi HS. 2014. Quality characteristics of fermented soybean products by Bacillus sp. isolated from traditional soybean paste. J Korean Soc Food Sci Nutr 43:756-762
  19. Medic J, Atkinson C, Hurburgh CR. 2014. Current knowledge in soybean composition. J Am Oil Chem Soc 91:363-384
  20. Moon HK, Lee SW, Moon JN, Kim DH, Yoon WJ, Kim GY. 2011. Quality characteristics of various beans in distribution. J East Asian Soc Diet Life 21:215-221
  21. Oh GS, Kang KJ, Hong YP, An YS, Lee HM. 2003. Distribution of organic acids in traditional and modified fermented foods. J Korean Soc Food Sci Nutr 32:1177-1185
  22. Park HM, Gu YR, Hong JH. 2016. Physicochemical properties and antioxidant activities of polysaccharides from Cheonggukjang and Pueraria thunbergiana-Cheonggukjang. J Chitin Chitosan 21:26-31
  23. Park MK, Choi HK, Kwon DY, Kim YS. 2007. Study of volatile organic acids in freeze-dried Cheonggukjang formed during fermentation using SPME and stable-isotope dilution assay (SIDA). Food Chem 105:1276-1280
  24. Park MK, Choi HS, Kim YS, Cho IH. 2017. Change in profiles of volatile compounds from two types of Fagopyrum esculentum (buckwheat) soksungjang during fermentation. Food Sci Biotechnol 26:871-882
  25. Shin DS, Park CH, Choi ID, Lee SK, Park JY, Kim NG, Choi HS. 2019. Quality properties of soy-paste soybean cultivar for fermented soybean products. Korean J Food Nutr 32:114-121
  26. Sirilun S, Sivamaruthi BS, Kesika B, Peerajan S, Chaiyasut C. 2017. Lactic acid bacteria mediated fermented soybean as a potent nutraceutical candidate. Asian Pac J Trop Biomed 7:930-936
  27. Son SJ, Lee SP. 2011. Evaluation of rheological and functional properties of roasted soybean flour and mixed cereals fermented by Bacillus sp. J Korean Soc Food Sci Nutr 40:450-457
  28. Woo KS, Lee JH, Lee BW, Lee YY, Lee BK, Kim HJ. 2018. Effect of germination and roasting treatment on the quality characteristics and antioxidant properties of black soybean flours. Food Eng Prog 22:75-84
  29. Yang J, Wu XB, Chen HL, Sun-waterhouse D, Zhong HB, Cui C. 2019. A value-added approach to improve the nutritional quality of soybean meal byproduct: Enhancing its antioxidant activity through fermentation by Bacillus amyloliquefaciens SWJS22. Food Chem 272:396-403
  30. Youn KC, Kim DH, Kim JO, Park BJ, Yook HS, Cho JM, Byun MW. 2002. Quality characteristics of the chungkookjang fermented by the mixed culture of Bacillus natto and B. lichniformis. J Korean Soc Food Sci Nutr 31:204-210
  31. Zheng YF, Jeong JK, Choi HS, Park KY. 2011. Increased quality characteristics and physiological effects of chunggukjang fermented with Bacillus subtilis-SKm. J Korean Soc Food Sci Nutr 40:1694-1699