Characteristics of Biorefinery Process Using Laminaria japonica for the Production of Carbohydrate and Reducing Sugar

Laminaria japonica를 이용하여 탄수화물과 환원당 생산을 위한 바이오리파이너리 공정 특성

  • Kim, Ji Hyun (CK Translational Research Center, CK Biotech) ;
  • Ha, Jeong Hyub (Department of Integrated Environmental Systems, Pyeongtaek University) ;
  • Choi, Suk Soon (Department of Biological and Environmental Engineering, Semyung University) ;
  • Park, Jong Moon (Department of Chemical Engineering, Pohang University of Science and Technology)
  • 김지현 ((주)씨케이바이오텍) ;
  • 하정협 (평택대학교 환경융합시스템학과) ;
  • 최석순 (세명대학교 바이오환경공학과) ;
  • 박종문 (포스텍 화학공학과)
  • Received : 2019.08.27
  • Accepted : 2019.09.17
  • Published : 2019.10.10


In this study, Laminaria japonica was used as a substrate for a mixed aerobic microbial consortium. Laminaria japonica is well-known as a representative brown algal biomass possessing advantages of cheap cost, and high productivity and carbohydrate content. A biological saccharification system was established by inoculating and enriching the mixed aerobic microbial consortium. Production of the soluble carbohydrate and reducing sugar at different hydraulic retention times (HRT) was observed. The efficiency of saccharification increased according to the decrease of HRT. The maximum saccharification yield in a continuous biological pretreatment process was 17.96 and 4.30 g/L/day for the soluble carbohydrate and reducing sugar, respectively at the HRT of 1 day. In contrast, the staccharification yield decreased drastically at the HRT of 0.5 day. Experimental results indicate that Laminaria japonica is a promising material for the production of useful products, in particular for the saccharification through a biorefinery process. It can thus be concluded that a continuous biological pretreatment process using a mixed cultivation system can be successfully employed for the biorefinery technology.


Supported by : 평택대학교


  1. P. S. Nigam and A. Singh, Production of liquid biofuels from renewable resources, Prog. Energy Combust. Sci., 37, 52-68 (2011).
  2. B. Dien, M. Cotta, and T. Jeffries, Bacteria engineered for fuel ethanol production: Current status, Appl. Microbiol. Biotechnol., 63, 258-266 (2003).
  3. K. A. Jung, S.-R. Lim, Y. Kim, and J. M. Park, Potentials of macroalgae as feedstocks for biorefinery, Bioresour. Technol., 135, 182-190 (2013).
  4. A. Hendriks and G. Zeeman, Pretreatments to enhance the digestibility of lignocellulosic biomass, Bioresour. Technol., 100, 10-18 (2009).
  5. Y. Sun and J. Cheng, Hydrolysis of lignocellulosic materials for ethanol production: A review, Bioresour. Technol., 83, 1-11 (2002).
  6. R. E. Sims, W. Mabee, J. N. Saddler, and M. Taylor, An overview of second generation biofuel technologies, Bioresour. Technol., 101, 1570-1580 (2010).
  7. P. Fasahati and J. J. Liu, Process simulation of bioethanol production from brown algae, Cellulose, 6, 6-13 (2012).
  8. H. R. Park, Production of Organic Acids from Seaweed Biomass (Laminaria japonica) using a Continuous Mixed Culture System, Graduate School of Pohang University of Science and Technology. MS Thesis, Pohang, Korea (2012).
  9. S. Fernando, S. Adhikari, C. Chandrapal, and N. Murali, Biorefineries: Current status, challenges, and future direction, Energy Fuel., 20, 1727-1737 (2006).
  10. H. A. Kim, Enterobacter sp. JMP3, a Potent Bacterium for the Production of Value Added Products from Marine Algal Biomass, Laminaria japonica, Pohang University of Science and Technology. MS Thesis, Pohang, Korea (2011).
  11. J. Cronshaw, A. Myers, and R. Preston, A chemical and physical investigation of the cell walls of some marine algae, Biochim. Biophys. Acta, 27, 89-103 (1958).
  12. F. A. Keller, J. E. Hamilton, and Q. A. Nguyen, Microbial pretreatment of biomass. Appl. Biochem. Biotech., 105, 27-41 (2003).
  13. L. Ge, P. Wang, and H. Mou, Study on saccharification techniques of seaweed wastes for the transformation of ethanol, Renew. Energy, 36, 84-89 (2011).
  14. APHA, Standard Methods for the Examination of Water and Wastewater, 20th ed., American Public Health Association (APHA), Washington DC, USA (1998).
  15. M. Dubois, K. A. Gilles, J. K. Hamilton, P. T. Rebers, and F. Smith, Colorimetric method for determination of sugars and related substances, Anal. Chem., 28, 350-356 (1956).
  16. R. Bottle and G. Gilbert, The use of alkaline reagents to determine carbohydrate reducing groups, Analyst, 83, 403-406 (1958).
  17. R. P. John, G. Anisha, K. M. Nampoothiri, and A. Pandey, Micro and macroalgal biomass: A renewable source for bioethanol, Bioresour. Technol., 102, 186-193 (2011).
  18. M. Daroch, S. Geng, and G. Wang, Recent advances in liquid biofuel production from algal feedstocks, Appl. Energy, 102, 1371-1381 (2013).
  19. H. M. Jang, J. H. Ha, J. M. Park, M. S. Kim, and S. G. Sommer, Comprehensive microbial analysis of combined mesophilic anaerobic-thermophilic aerobic process treating high-strength food wastewater, Water Res., 73, 291-303 (2015).