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Anti-inflammatory Efficacy and Liver Protective Activity of Pine Pollen according to Probe Sonicator Ultrasonic Disintegration Extraction Method

송화분의 초음파 파쇄 추출 방법에 따른 항염증 효능 및 간 보호 활성

  • Kim, Ok Ju (Department of Natural Science Institute, Silla University) ;
  • Woo, Young Min (Department of Natural Science Institute, Silla University) ;
  • Jo, Eun Sol (Department of Natural Science Institute, Silla University) ;
  • Jo, Min Young (Department of Pharmaceutical Engineering, Silla University) ;
  • Li, Chun-Ri (College of Acupuncture and Moxibustion, Liaoning University of Traditional Chinese Medicine) ;
  • Lee, Young-Ho (Protein Structure Research Group, Division of Bioconvergence Analysis, Korea Basic Science Institute, Bio-Analytical Science, University of Science and Technology (UST)) ;
  • Ahn, Mee Young (Department of Pharmaceutical Engineering, Silla University) ;
  • Lee, Sang-Hyeon (Department of Pharmaceutical Engineering, Silla University) ;
  • Ha, Jong Myung (Department of Pharmaceutical Engineering, Silla University) ;
  • Kim, Andre (Department of Pharmaceutical Engineering, Silla University)
  • 김옥주 (신라대학교 자연과학연구소) ;
  • 우영민 (신라대학교 자연과학연구소) ;
  • 조은솔 (신라대학교 자연과학연구소) ;
  • 조민영 (신라대학교 제약공학전공) ;
  • 이춘일 (중국 센양 요녕중의대) ;
  • 이영호 (한국기초과학연구소, 과학기술연합대학원) ;
  • 안미영 (신라대학교 제약공학전공) ;
  • 이상현 (신라대학교 제약공학전공) ;
  • 하종명 (신라대학교 제약공학전공) ;
  • 김안드레 (신라대학교 제약공학전공)
  • Received : 2019.07.09
  • Accepted : 2019.08.13
  • Published : 2019.10.10

Abstract

In this study, the effect anti-oxidant, anti-inflammatory, and liver protective activity was investigated via quick ultrasonic disintegration of pine pollen using a probe sonicator (PS) followed by the extraction with water, 70% ethanol, and 100% ethanol. The anti-inflammatory effect was studied by measuring the production of nitric oxide (NO) and cytokine in RAW264.7 cells induced with lipopolysaccharides (LPS). The cell toxicity was also checked with an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the experiment was conducted using non-toxic $100{\mu}g/mL$. The NO inhibition rate was highest in the 70% ethanol PS group at $85.99{\pm}0.12%$. Also an excellent efficiency was obtained from the results of interlukin-1 beta ($IL-1{\beta}$) and tumor necrosis factor alpha ($TNF-{\alpha}$), which is related to inflammation-related cytokine, with the respective inhibition rates of 63 and 22%. To examine liver protective activity, HepG2 cells were treated with Taclin, and the generation of glutamic oxaloacetic transaminase (GOT) and lactate dehydrogenase (LDH) was measured in the culture solution. From GOT and LDH generation results, the inhibition rates in the 70% ethanol PS group were 28% and 13%, respectively, which was higher compared to that of using negative control group. Our results suggest that pine pollen extracted in 70% ethanol using PS may be used to develop food products that have anti-aging, anti-inflammatory, and liver protective effects.

Acknowledgement

Supported by : 중소벤처기업부

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