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Complete genome sequence of Celluosilyticum lentocellum WCF-2 isolated from cow dung

소 분변에서 분리된 Celluosilyticum lentocellum WCF-2의 유전체 염기서열 분석

Heo, Jun;You, Jaehong;Park, InCheol;Han, Byeong-Hak;Kwon, Soon-Wo;Ahn, Jae-Hyung
허준;유재홍;박인철;한병학;권순우;안재형

  • Received : 2019.09.09
  • Accepted : 2019.09.18
  • Published : 2019.09.30

Abstract

An anaerobic bacterial strain WCF-2 was isolated from cow dung in finding cellulose-degrading bacteria for use as silage additives. Strain WCF-2 showed a higher cellulolytic activity than Cellulosilyticum lentocellum DSM $5427^T$, the closest relative of strain WCF-2 (98.2% of 16S rRNA gene sequence similarity). We sequenced the complete genome of strain WCF-2 and compared it with that of C. lentocellum DSM $5427^T$. The OrthoANI value between the two strains was 97.9% thus strain WCF-2 was identified as C. lentocellum. The genome size of strain WCF-2 was 4,779,774 bp with a G + C content of 34.4%, 4,154 coding genes (CDS), 54 pseudo genes, and 142 RNA genes. Strain WCF-2 harbored seven cellulase genes, five of which showed low similarities with those of C. lentocellum DSM $5427^T$.

Keywords

Cellulosilyticum lentocellum;cellulolytic activity;genome sequence

Acknowledgement

Supported by : National Institute of Agricultural Sciences