Complete genome sequence of Enterococcus faecalis strain DM01, a potential starter culture candidate for soybean fermentation

콩발효 종균후보 Enterococcus faecalis strain DM01의 유전체 염기서열

  • Heo, Sojeong (Department of Food and Nutrition, Dongduk Women's University) ;
  • Lee, Jong-Hoon (Department of Food Science and Biotechnology, Kyonggi University) ;
  • Jeong, Do-Won (Department of Food and Nutrition, Dongduk Women's University)
  • 허소정 (동덕여자대학교 식품영양학과) ;
  • 이종훈 (경기대학교 식품생물공학과) ;
  • 정도원 (동덕여자대학교 식품영양학과)
  • Received : 2019.08.06
  • Accepted : 2019.09.03
  • Published : 2019.09.30


Enterococcus faecalis strain DM01 was isolated from meju, a traditional Korean fermented soybean product. The strain did not exhibit resistance to eight antibiotics (ampicillin, chloramphenicol, ciprofloxacin, erythromycin, gentamicin, penicillin G, tetracycline, and vancomycin), biofilm formation, and hemolytic activity. The genome of strain DM01 consists a single circular 2,785,968-bp chromosome with a G + C content of 37.68%. The complete genomic sequence of strain DM01 provides genetic information to support the absence of antibiotic resistance, biofilm formation, and hemolytic activity.


Enterococcus faecalis;meju;soybean fermentation;starte


Supported by : National Research Foundation of Korea (NRF)


  1. EFSA. 2004. EFSA scientific colloquium. Summary report. QPS. Qualified presumption of safety of microorganism in food and feed. Brussels, Belgium.
  2. Foulquie Moreno MR, Sarantinopoulos P, Tsakalidou E, and De Vuyst L. 2006. The role and application of enterococci in food and health. Int. J. Food Microbiol. 106, 1-24.
  3. Franz CM, Huch M, Abriouel H, Holzapfel W, and Galvez A. 2011. Enterococci as probiotics and their implications in food safety. Int. J. Food Microbiol. 151, 125-140.
  4. Jeong DW, Heo S, Ryu S, Blom J, and Lee JH. 2017. Genomic insights into the virulence and salt tolerance of Staphylococcus equorum. Sci. Rep. 7, 5383.
  5. Jeong DW, Kim HR, Jung G, Han S, Kim CT, and Lee JH. 2014. Bacterial community migration in the ripening of doenjang, a traditional Korean fermented soybean food. J. Microbiol. Biotechnol. 24, 648-660.
  6. Park J, Jin GD, Pak JI, Won J, and Kim EB. 2017. Development of a rapid identification method for the differentiation of Enterococcus species using a species-specific multiplex PCR based on comparative genomics. Curr. Microbiol. 74, 476-483.
  7. Tatusov RL, Koonin EV, and Lipman DJ. 1997. A genomic perspective on protein families. Science 278, 631-637.
  8. Tatusova T, DiCuccio M, Badretdin A, Chetvernin V, Nawrocki EP, Zaslavsky L, Lomsadze A, Pruitt KD, Borodovsky M, and Ostell J. 2016. NCBI prokaryotic genome annotation pipeline. Nucleic Acids Res. 44, 6614-6624.
  9. Yamaguchi T, Miura Y, and Matsumoto T. 2013. Antimicrobial susceptibility of Enterococcus strains used in clinical practice as probiotics. J. Infect. Chemother. 19, 1109-1115.