Characterization of Miniimonas sp. S16 isolated from activated sludge

활성슬러지로부터 분리된 Miniimons sp. S16 세균의 특성

  • 고현우 (비타바이오 생물자원개발연구소) ;
  • 김홍익 (비타바이오 생물자원개발연구소) ;
  • 박수제 (제주대학교 생물학과)
  • Received : 2019.07.05
  • Accepted : 2019.08.01
  • Published : 2019.09.30


Biological factors (e.g. microorganism activity) in wastewater treatment plant (WWTP) play essential roles for degradation and/or removal of organic matters. In this study, to understand the microbial functional roles in WWTP, we tried to isolate and characterize a bacterial strain from activated sludge sample. Strain S16 was isolated from the activated sludge of a municipal WWTP in Daejeon metropolitan city, the Republic of Korea. The cells were a Gram-stain-positive, non-motile, facultative anaerobe, and rod-shaped. Strain S16 grew at a temperature of $15{\sim}40^{\circ}C$ (optimum, $30^{\circ}C$), with 0~9.0% (w/v) NaCl (optimum, 1.0~2.0%), and at pH 5.5~9.0 (optimum, pH 7.0~7.5). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S16 was most closely related to the unique species Miniimonas arenae NBRC $106267^T$ (99.79%, 16S rRNA gene sequence similarity) of the genus Miniimonas. The cell wall contained alanine, glutamic acid, serine, and ornithine. Although the isolation source of the type strain NBRC $106267^T$ which considered as a marine microorganism is sea sand, that of strain S16 is terrestrial environment. It might raise an ecological question for habitat transition. Therefore, comparative genome analysis will be valuable investigation for shedding light on their potential metabolic traits and genomic streamlining.


Miniimonas;polyphasic analysis;sludge


Supported by : Jeju National University


  1. Bachy C and Worden AZ. 2014. Microbial Ecology: Finding Structure in the Rare Biosphere. Curr. Biol. 24, 315-317.
  2. Ezaki T, Hashimoto Y, and Yabuuchi E. 1989. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotope are used to determine genetic relatedness among bacterial strains. Int. J. Syst. Bacteriol. 39, 224-229.
  3. Felsenstein J. 1981. Evolutionary trees from DNA sequences: a maximum likelihood approach. J. Mol. Evol. 17, 368-376.
  4. Fitch WM. 1971. Toward defining the course of evolution: minimum change for a specific tree topology. Syst. Biol. 20, 406-416.
  5. Gonzalez JM and Saiz-Jimenez C. 2002. A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ. Microbiol. 4, 770-773.
  6. Hu HY, Fujie K, and Urano K. 1999. Development of a novel solid phase extraction method for the analysis of bacterial quinones in activated sludge with a higher reliability. J. Biosci. Bioeng. 87, 378-382.
  7. Kimura M. 1989. The neutral theory of molecular evolution and the world view of the neutralists. Genome 31, 24-31.
  8. Koh HW, Hong H, Min UG, Kang MS, Kim SG, Na JG, Rhee SK, and Park SJ. 2015. Rhodanobacter aciditrophus sp. nov., an acidophilic bacterium isolated from mine wastewater. Int. J. Syst. Evol. Microbiol. 65, 4574-4579.
  9. Koh HW, Rani S, Kim SJ, Moon E, Nam SW, Rhee SK, and Park SJ. 2017. Halomonas aestuarii sp. nov., a moderately halophilic bacterium isolated from a tidal flat. Int. J. Syst. Evol. Microbiol. 67, 4298-4303.
  10. Kumar S, Stecher G, and Tamura K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol. Biol. Evol. 33, 1870-1874.
  11. Martin C and Vanrolleghem PA. 2014. Analysing, completing, and generating influent data for WWTP modelling: A critical review. Environ. Model Softw. 60, 188-201.
  12. Menendez E, Flores-Felix JD, Mulas R, Andres FG, Fernandez-Pascual M, Peix A, and Velazquez E. 2017. Paenibacillus tritici sp. nov., isolated from wheat roots. Int. J. Syst. Evol. Microbiol. 67, 2312-2316.
  13. Saitou N and Nei M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425.
  14. Schleifer KH and Kandler O. 1972. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol. Rev. 36, 407-477.
  15. Stolp H. 1988. Microbial Ecology: Organisms, Habitats, Activities. Cambridge: Cambridge University Press, England.
  16. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, and Higgins DG. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25, 4876-4882.
  17. Ue H, Matsuo Y, Kasai H, and Yokota A. 2011. Miniimonas arenae gen. nov., sp. nov., an actinobacterium isolated from sea sand. Int. J. Syst. Evol. Microbiol. 61, 123-127.
  18. Webley DM. 1953. A simple method for producing microcultures in hanging drops with special reference to organisms utilizing oils. J. Gen. Microbiol. 8, 66-71.
  19. Weisburg WG, Barns SM, Pelletier DA, and Lane DJ. 1991. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173, 697-703.
  20. Yashroy RC. 1990. Lamellar dispersion and phase separation of chloroplast membrane lipids by negative staining electron microscopy. J. Biosci. 15, 93-98.
  21. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y, Seo H, and Chun J. 2017. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int. J. Syst. Evol. Microbiol. 67, 1613-1617.
  22. Zhang H, Yoshizawa S, Sun Y, Huang Y, Chu X, Gonzalez JM, Pinhassi J, and Luo H. 2019. Repeated evolutionary transitions of flavobacteria from marine to non-marine habitats. Environ. Microbiol. 21, 648-666.