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Comparison between DNA- and cDNA-based gut microbial community analyses using 16S rRNA gene sequences

16S rRNA 유전자 서열 분석을 이용한 DNA 및 cDNA 기반 장내 미생물 군집 분석의 비교

  • Jo, Hyejun (Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University) ;
  • Hong, Jiwan (Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University) ;
  • Unno, Tatsuya (Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University)
  • 조혜준 (제주대학교 생명자원과학대학 생명공학부) ;
  • 홍지완 (제주대학교 생명자원과학대학 생명공학부) ;
  • 운노타쯔야 (제주대학교 생명자원과학대학 생명공학부)
  • Received : 2019.06.14
  • Accepted : 2019.07.05
  • Published : 2019.09.30

Abstract

Studies based on microbial community analyses have increased in the recent decade since the development of next generation sequencing technology. Associations of gut microbiota with host's health are one of the major outcomes of microbial ecology filed. The major approach for microbial community analysis includes the sequencing of variable regions of 16S rRNA genes, which does not provide the information of bacterial activities. Here, we conducted RNA-based microbial community analysis and compared results obtained from DNA- and its cDNA-based microbial community analyses. Our results indicated that these two approaches differed in the ratio of Firmicutes and Bacteroidetes, known as an obesity indicator, as well as abundance of some key bacteria in gut metabolisms such as butyrate producers and probiotics strains. Therefore, cDNA-based microbial community may provide different insights regarding roles of gut microbiota compared to the previous studies where DNA-based microbial community analyses were performed.

Keywords

cDNA;gut microbiota;microbial community analysis;miSeq

Acknowledgement

Supported by : Jeju National University

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