DOI QR코드

DOI QR Code

Suppressive effect of Senecio iscoensis Hieron. extract in Propionibacterium acnes-induced inflammatory signaling pathway

Senecio iscoensis Hieron. 추출물의 Propionibacterium acnes에 의한 염증반응 억제효과

  • Shin, Jin Hak (Department of Molecular Bioscience, College of Biomedical Science, Institute of Bioscience & Biotechnology, Kangwon National University) ;
  • Lee, Eun Hye (Department of Molecular Bioscience, College of Biomedical Science, Institute of Bioscience & Biotechnology, Kangwon National University) ;
  • Kim, Seon Sook (Department of Molecular Bioscience, College of Biomedical Science, Institute of Bioscience & Biotechnology, Kangwon National University) ;
  • Yi, Dong-Keun (International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Roh, Jin Kyung (Instituto de BioEconomia, El Batan) ;
  • Seo, Su Ryeon (Department of Molecular Bioscience, College of Biomedical Science, Institute of Bioscience & Biotechnology, Kangwon National University)
  • 신진학 (강원대학교 분자생명과학과) ;
  • 이은혜 (강원대학교 분자생명과학과) ;
  • 김선숙 (강원대학교 분자생명과학과) ;
  • 이동근 (한국생명공학연구원) ;
  • 노진경 (생물경제연구소 에콰도르) ;
  • 서수련 (강원대학교 분자생명과학과)
  • Received : 2019.06.10
  • Accepted : 2019.06.23
  • Published : 2019.09.30

Abstract

Propionibacterium acnes (P. acnes) lives in the hair follicles and pores, and it uses cell debris, sebum and metabolic byproducts of surrounding skin tissues as energy and nutrients. Increased production of sebum due to sebaceous hyperplasia or blockage of the follicle can cause growth and proliferation of P. acnes. The rapid growth of P. acnes in follicles produces cell damage, metabolic byproducts and bacterial chips, which can cause inflammation. In this study, we examined the possibility of Senecio iscoensis Hieron. (S. iscoensis) extract to regulate P. acnes-induced inflammatory signaling pathways. We observed that S. iscoensis extract effectively inhibited P. acnes-induced pro-inflammatory cytokine expressions such as IL-$1{\beta}$, TNF-${\alpha}$, and iNOS in mouse macrophage cell line Raw 264.7. The inhibitory effect of S. iscoensis in pro-inflammatory cytokine levels was accompanied by the inhibition of the transcription factors NF-${\kappa}B$ and NF-AT. However, S. iscoensis did not alter the P. acnes-induced MAPK signaling pathways. This study first suggests the potential of using S. iscoensis extract as an alternative agent for the treatment of acne.

Keywords

Propionibacterium acnes;S. iscoensis Hieron.;acne;inflammation;pro-inflammatory cytokine

Acknowledgement

Supported by : 강원대학교, 한국연구재단

References

  1. Brown JM and Poston SM. 1983. Resistance of propionibacteria to antibiotics used in the treatment of acne. J. Med. Microbiol. 16, 271-280. https://doi.org/10.1099/00222615-16-3-271
  2. Chen Q, Koga T, Uchi H, Hara H, Terao H, Moroi Y, Urabe K, and Furue M. 2002. Propionibacterium acnes-induced IL-8 production may be mediated by NF-${\kappa}B$ activation in human monocytes. J. Dermatol. Sci. 29, 97-103. https://doi.org/10.1016/S0923-1811(02)00013-0
  3. Grange PA, Raingeaud J, Calvez V, and Dupin N. 2009. Nicotinamide inhibits Propionibacterium acnes-induced IL-8 production in keratinocytes through the NF-${\kappa}B$ and MAPK pathways. J. Dermatol. Sci. 56, 106-112. https://doi.org/10.1016/j.jdermsci.2009.08.001
  4. Jugeau S, Tenaud I, Knol A, Jarrousse V, Quereux G, Khammari A, and Dreno B. 2005. Induction of toll like receptors by Propionibacterium acnes. Br. J. Dermatol. 153, 1105-1113. https://doi.org/10.1111/j.1365-2133.2005.06933.x
  5. Kim J, Ochoa MT, Krutzik SR, Takeuchi O, Uematsu S, Legaspi AJ, Brightbill HD, Holland D, Cunliffe WJ, and Akira S. 2002. Activation of toll-like receptor 2 in acne triggers inflammatory cytokine responses. J. Immunol. 169, 1535-1541. https://doi.org/10.4049/jimmunol.169.3.1535
  6. Libermann TA and Baltimore D. 1990. Activation of interleukin-6 gene expression through the NF-${\kappa}B$ transcription factor. Mol. Cell. Biol. 10, 2327-2334. https://doi.org/10.1128/MCB.10.5.2327
  7. Lim YS, Myung KB, Chung NE, and Chung WS. 1995. A study on the MIC of antibiotics for Propionibacterium acnes in patients with acne. Korean J. Dermatol. 33, 437.
  8. Liu T, Zhang L, Joo D, and Sun SC. 2017. NF-${\kappa}B$ signaling in inflammation. Signal Transduct. Target. Ther. 2, 17023. https://doi.org/10.1038/sigtrans.2017.23
  9. Nakatsuji T, Kao MC, Fang JY, Zouboulis CC, Zhang L, Gallo RL, and Huang CM. 2009. Antimicrobial property of lauric acid against Propionibacterium acnes: Its therapeutic potential for inflammatory acne vulgaris. J. Invest. Dermatol. 129, 2480-2488. https://doi.org/10.1038/jid.2009.93
  10. Pan MG, Xiong Y, and Chen F. 2013. NFAT gene family in inflammation and cancer. Curr. Mol. Med. 13, 543-554. https://doi.org/10.2174/1566524011313040007
  11. Park KY. 2018. Pharmacological treatment options for acne. J. Korean Med. Association 61, 680-686. https://doi.org/10.5124/jkma.2018.61.11.680
  12. Suh DH. 2010. Pharmacologic treatment of acne. J. Korean Med. Assoc. 53, 623-629. https://doi.org/10.5124/jkma.2010.53.7.623
  13. Swanson JK. 2003. Antibiotic resistance of Propionibacterium acnes in acne vulgaris. Dermatol. Nurs. 15, 359-363.
  14. Vowels BR, Yang S, and Leyden JJ. 1995. Induction of proinflammatory cytokines by a soluble factor of Propionibacterium acnes: Implications for chronic inflammatory acne. Infect. Immun. 63, 3158-3165.
  15. Wang YY, Ryu AR, Jin S, Jeon YM, and Lee MY. 2017. Chlorin e6-mediated photodynamic therapy suppresses P. acnes-induced inflammatory response via $NF{\kappa}B$ and MAPKs signaling pathway. PLoS One 12, e0170599. https://doi.org/10.1371/journal.pone.0170599
  16. Zouboulis CC. 2001. Is acne vulgaris a genuine inflammatory disease? Dermatology 203, 277-279. https://doi.org/10.1159/000051771